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Biomedical subjects

P Mocarelli

Publications and source records attributed to P Mocarelli.

At least 55 records · Page 3Linked to original sources
PubMed1982

Potential 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure of Seveso decontamination workers: a controlled prospective study.

In 1976, an explosion at the Industrie Chimiche-Meda-Società Azionaria (ICMESA) plant in Meda, near the Italian town of Seveso, released a cloud of aerosol containing 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which contaminated the surrounding area. Clean-up of the highly polluted area of Seveso started on May 1980: in order to avoid exposure to significant levels of TCDD, safety measures are being used, but a potential exposure cannot be ruled out. A prospective study was set up at the beginning of the clean-up procedures, and laboratory tests are being used to evaluate trends among the decontamination workers. A reference group who met eligibility criteria at the preemployment examination was selected from employees of the same firm employing the decontamination was selected from employees of the same firm employing the decontamination workers. Medical examinations and laboratory tests have been performed on both the exposed and nonexposed groups at the same facilities. Analysis of the first follow-up period did not show any remarkable changes in a battery of laboratory tests of the exposed and nonexposed groups.

Adult↗
PubMed1981

Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells.

The effect of normal mouse marrow preincubation with Ehrlich tumor fluid (EAF) and Ehrlich tumor cell-conditioned medium (ET-CM) was investigated both on the in vivo assay for pluripotential stem cells (CFU-S) and on the in vitro assay for granulocytic-macrophagic progenitors (CFU-C). A significant reduction of the number of CFU-S was found when bone marrow cells were incubated with EAF. The morphological analysis showed a relative increase in the granulocytic type colonies besides an absolute increase in undifferentiated ones and an absolute decrease in erythroid colonies. The in vitro data show a decrease in CFU-C production, by stimulation with standard Colony Stimulating Factor (CSF), when the marrow cells are preincubated with EAF and ET-CM. When standard CAF (obtained from L-cells) is used at the higher concentrations, the CFU-C in the ET-CM treated marrow increases to the control values. EAF and ET-CM incubation also affects the CFU-C differentiation by increasing the number of pure granulocytic clones. The experimental data were discussed in order to explain an hypothesis about the activity of tumor-related factors.

Animals↗
PubMed1976

T-cell precursors in mice bearing the Ehrlich ascites tumors.

Mice bearing Ehrlich ascites tumors and challenged with sheep erythrocytes produced fewer plaque-forming cells than did normal mice. At the same time the immunosuppression developed, the number of T lymphocytes in the thymus and spleen were reduced significantly. In the spleen, the number of B lymphocytes remained constant during carcinogenesis, whereas that of the macrophages increased significantly, as compared to the controls. In this paper, we demonstrated that the mechanism responsible for thymus and spleen depletion of theta antigen-bearing cells had to be ascribed to fewer T-lymphocyte precursors in the bone marrow of mice with cancer. The reduction of T-lymphocyte precursors was probably caused by the same "soluble factor(s)" produced by Ehrlich ascites tumor cells, which also interfered with the proliferation of myelopoietic stem cells in the bone marrow of mice with this neoplasm, as we previously reported. By performing several reconstitution experiments of lethally X-irradiated hosts, we determined that the immunodepression by Ehrlich ascites tumor cells was readily reversible, and the alteration of the T:B lymphocyte ratio in the spleen had a minor function, if any, in the pathogenesis of the immunosuppression.

Animals↗
PubMed1975

A case of pneumonia associated with cytomegalovirus (CMV) and anti- CMV antibody detection.

A case of pneumonia associated with acute CMV infection is reported. A 34 year-old man was admitted with fever (39 degrees C equals 102.2 degrees F), malaise and dyspnea. Clinical findings were characteristic of viral pneumonia. Crepitant rales to chest auscultation were heard all over the lung. X-ray picture showed evidence of interstitial pneumonia. Leucocyte counts were normal whilst lymphocyte counts elevated. Bone marrow examination showed an increase of lymphocytes and lymphoblasts. To palpation liver and spleen were slightly enlarged. Liver function tests were slightly abnormal. Bacteriological tests on urine and blood and Paul-Bunnel test were negative. Antibiotic therapy was ineffective. CMV was isolated from saliva and urine. Serological studies (elevated CF and precipitin antibody titer) suggested the diagnosis of a CMV infection. The patient recovered in 3 weeks.

Adult↗
PubMed1972

Antibody response in vitro to an animal virus: production of rabies virus neutralizing antibodies by mouse cells in culture.

Rabies virus neutralizing antibodies were produced in vitro by the exposure of mouse spleen cells to live and inactivated rabies virus suspensions and to sheep erythrocytes coated with rabies virus. These antibodies did not neutralize two other rhabdoviruses: Kern Canyon and vesicular stomatitis viruses, and were precipitable by treatment with an antiserum to mouse IgG. Removal of "glass-adhering" cells from mouse spleen cell suspensions abolished the antibody response, which could be restored by the addition of mouse peritoneal exudate cells, rich in macrophages.

Animals↗