Biomedical subjects

P Minárik

Publications and source records attributed to P Minárik.

5 recordsLinked to original sources

PubMed2004

Insulin gene profile cycles with season of birth of future diabetic children and their relatives.

BACKGROUND: HLA class II gene pattern and IA-2A antibody positivity, revealing selected risks for prediction of type 1 diabetes mellitus (DM1), were previously shown to be cycling roughly in parallel with seasonally fluctuating frequencies of births of future diabetic children. Is this also true for insulin genotyping? Is this also true for births of the healthy close relatives of children with DM1? PATIENTS AND METHODS: In 98 Slovak children with DM1 and in 60 healthy parents and siblings, two single nucleotide polymorphisms of the insulin gene (-23 Hph I; +1127 Pst I) were typed. Results were expressed as score 0 (maximal risk) up to 2 (minimal risk or protection), or 0-4 for the sum of both scores. Birth seasonality of these risk scores was tested by Halberg cosinor regression. RESULTS: Results were similar for the two alleles; therefore, the summed risk score was evaluated. The DM1 risk from the insulin gene pattern in births of future diabetics cycled significantly semi-annually, with maxima around the spring and autumn equinoxes. In the relatives, a significant annual and extremely pronounced quarterly peaking rhythm was found, with maximum risk in births around the autumn equinox and shortly after the winter solstice. Significant protection peaks are present in late winter, spring and summer. CONCLUSION: The highest frequency of Coxsackie infections in the Slovakian child population, and of 'diabetic' births and manifestations, found earlier together with maximal risks from HLA II and IA-2A antibodies in late summer and early autumn, is now confirmed also for the insulin gene pattern in diabetic children and even also for their healthy close relatives.

Adult↗

PubMed2004

Trends in the incidence of childhood-onset type 1 diabetes in Slovakia 1985 - 2000.

The aim of this work was to study and monitor the patterns in incidence of childhood type 1 diabetes in Slovakia. The incidence of type 1 diabetes per 100,000/year from 1985 to 2000 was determined in children 0 - 14 years old. A total of 1,818 cases were diagnosed during this time. Poisson regression models were used to study incidence trends. The overall incidence of type 1 diabetes varied from 5.62/100,000 per year in year 1986 to 14.46/100,000 in year 1998. This represents a 2.57-fold increase. The incidence increased with age and was the highest among children 10 - 14 years of age. The incidence of type 1 diabetes in Slovakia is increasing very dramatically, especially in age group 0 - 4 years.

Adolescent↗

PubMed2002

Malate dehydrogenases--structure and function.

Malate dehydrogenases (MDH, L-malate:NAD oxidoreductase, EC 1.1.1.37), catalyze the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate/aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. They are homodimeric molecules in most organisms, including all eukaryots and the most bacterial species. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure.

Animals↗

PubMed1994

[Endoscopic therapy of biliary tract diseases].

Endoscopy plays a dominant role in the diagnosis and treatment of biliary tract disease and disease of pancreas as well. The authors of the article present their own experience with the endoscopic treatment of these diseases which they have obtained since 1982 by performing endoscopic papilosphincterotomy in 2000 patients (1340 female and 660 male patients). The most common indication for this treatment was choledocholithiasis and benign stenosis of Vater's papila (or dysfunction of Oddi's sphincter). They succeeded in 97.9%, the worst results were in patients after the B II-resection of the stomach. The occurrence of complications was 3.4% in the entire group of patients, mortality was 0.5%. In the next part of the article the authors present other methods of treatment of choledocholithiasis and other diseases of biliary a pancreatic duct system (extraction of biliary stones, lithotripsy, biliary and pancreatic drainage). According to the literary data and own experience the endoscopic treatment of biliary diseases is highly effective and relatively safe when performed by experienced specialists. (Tab. 2, Ref. 16.)

Adolescent↗

PubMed1993

Influence of the different amino acid substitutions in Escherichia coli thioredoxin on the growth of bacteriophages T7 and f1.

We have constructed three mutants in the thioredoxin (trxA) gene changing its catalytic core between Cys-32 and Cys-35. Oligonucleotide-directed mutagenesis was carried out to replace conservative Gly-33 or Pro-34 by leucine, lysine, glutamine, phenylalanine or tryptophane. The mutants were characterized using an in vivo assay based on the ability of cell (mutants in the chromosomal trxA gene) to support growth of T7 and filamentous f1 phages. The results indicate that the smaller group side-chain in the position 33 and 34 of amino acid residues are indispensable for the growth of phages.

Bacteriophage T7↗