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Biomedical subjects

P Mahieu

Publications and source records attributed to P Mahieu.

262 records · Page 15Linked to original sources
PubMedDate not supplied

Tissue culture of normal rat glomeruli: characterization of collagenous and non-collagenous basement membrane antigens on the epithelial and mesangial cells.

Immunofluorescent studies were performed on epithelial and mesangial cells derived from normal rat glomeruli, using specific antisera directed against insoluble glomerular basement membranes (GMB), or directed against either type IV procollagen or non-collagenous antigens purified from basement membrane polypeptides synthesized by a murine tumour. In marked contrast to the mesangial cells, the epithelial cells contained large amounts of GBM antigens, of type IV procollagen, and of non-collagenous glycoproteins in the first two days after subculture plating. At the same time, the mesangial cells did not exhibit any basement membrane antigens. Five to 10 days later, however, all the antisera tested stained the surface membrane and/or the extracellular material of both epithelial and mesangial cells. The data suggest that both cell lines synthesize and secrete cross-reacting type IV procollagen and non-collagenous basement membrane antigenic material into the culture medium.

Animals↗
PubMedDate not supplied

[The adult respiratory distress syndrome. Clinical signs, radiology and biological data (author's transl)].

The etiology of shocklung is diverse. All causes lead to a lesion of the alveolar-capillary membrane with interstitial and subsequent alveolar edema. The clinical manifestations, although variable, are progressive and can be divided into 3 stages, which are the expression of a decrease in pulmonary compliance and of hypoxemia due to a ventilation-perfusion defect (shunt effect). The diagnosis of shocklung must be differentiated from pulmonary edema of cardiac origin by right heart catheterization and demonstrate a normal capillary pressure, a pulmonary hypertension with increased pulmonary resistance and a normal or increased cardiac output. A decreased PaO2 is the first sign of a ventilation-perfusion imbalance. The problem of alveolar-capillary O2 transfer is better defined by the alveolar-arterial O2 difference, the calculation of quantity of the intrapulmonary shunt and the hyperoxia curve. The daily analysis of the hyperoxia curve allows for a better appreciation of the clinical status than the clinical and radiological signs. The prognosis of the shockening is poor inspite of better reanimation technics. The mortality is about 50% and depends on 3 factors: etiology, early diagnosis and correction of the primary insult.

Adult↗
PubMedDate not supplied

[Antineutrophil cytoplasmic antibodies (ANCA) in inflammatory diseases of the digestive tract].

Antineutrophil cytoplasmic antibodies have recently been demonstrated in sera of patients with inflammatory bowel disease. We are reporting here our experience among 94 patients with Crohn's disease and 25 with ulcerative colitis. The indirect immunofluorescence assay with alcohol fixed neutrophils has been used. The presence of antineutrophil cytoplasmic antibodies was significantly associated to the ulcerative colitis 61% VS 17% (p < 0.001). The fluorescence had a perinuclear pattern among all but one suffering from ulcerative colitis. Among the patients suffering from ulcerative colitis there was no relationship between the presence of antineutrophil cytoplasmic antibodies and the activity or the localisation of the disease. Among the 9 patients operated for their ulcerative colitis, 4 were ANCA positive and remained so after proctocolectomy. The existence of antineutrophil cytoplasmic antibodies is useful for the diagnosis of some unclassifiable colitis. Their presence may be used as an argument in favour of some disturbances of the immunoregulation of the disease.

Adult↗
PubMedDate not supplied

Cytogenetic observations after meglumine antimoniate therapy for visceral leishmaniasis.

Few data are available concerning the genotoxic effects of antimonial salts therapy in humans. A patient suffering from visceral leishmaniasis was treated for 15 days with a cumulative dose meglumine antimoniate 42.5 g. Peripheral blood lymphocytes sampled before treatment, 7 days later, and at the end of therapy (day 15) were examined for the presence of structural chromosome aberrations, sister chromatid exchanges (SCEs), and micronuclei in binucleated cells. The treatment resulted in an increase of binucleated cells carrying micronuclei, with no changes in chromosome structural aberrations or in mean SCE frequency. On the basis of these observations and of experimental results reported in the literature, we conclude that therapy with meglumine antimoniate apparently does not represent a mutagenic or carcinogenic risk to humans.

Animals↗