Purification and characterization of a second form of hepatic cytochrome P-448 from rats treated with a pure polychlorinated biphenyl isomer.
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Animals↗
Biomedical subjects
P Linko
Publications and source records attributed to P Linko.
Structure-activity relationships of chlorinated benzenes as inducers of different forms of cytochrome P-450 in rat liver.
Hexachlorobenzene (HCB) produced increases in ethoxyresorufin (ERR) O-deethylase, aryl hydrocarbon hydroxylase (AHH) and aminopyrine N-demethylase activities in rat liver phenobarbital which were intermediate between those produced by phenobarbital and 3,4-benzpyrene (BP), alpha-Naphthoflavone (ANF) selectivity inhibited ERR activity in BP and HCB-induced microsomes (94% and 88%). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of liver microsomes indicated that HCB did not produce a detectable increase in a polypeptide with electrophoretic properties similar to those of purified cytochrome P-448 (Mr = 56,000). However, HCB did induce a polypeptide with Mr = 53,000 corresponding to one of two polypeptide bands induced by BP. This polypeptide may represent a second form of cytochrome P-448. Purification of HCB to remove possible dibenzo-p-dioxin impurities did not alter the 'mixed-type' induction produced by HCB. In contrast to HCB, all other chlorinated benzenes tested resembled phenobarbital as inducers.
Aminopyrine N-Demethylase↗
Multiple forms of solubilized and partially resolved cytochrome P-450 from rats induced by 2,3,5,2',3',5'- and 3,4,5,3',4',5'-hexachlorobiphenyls.
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Animals↗
Marked differences in the inductive effects of two symmetrical hexachlorobiphenyls and the corresponding unsymmetrical isomer on hepatic monooxygenases.
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Animals↗
On the Nature of Glutamic Acid Decarboxylase in Wheat Embryos.
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Journal Article↗
Enzyme Activation in Wheat Grains in Relation to Water Content. Glutamic Acid-Alanine Transaminase, and Glutamic Acid Decarboxylase.
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Journal Article↗
2,3,7,8-Tetrachlorodibenzofuran in a commercially available 99% pure polychlorinated biphenyl isomer identified as the inducer of hepatic cytochrome P-448 and aryl hydrocarbon hydroxylase in the rat.
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Animals↗