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Biomedical subjects

P Leone

Publications and source records attributed to P Leone.

100 records · Page 6Linked to original sources
PubMedDate not supplied

Substrate characterization for underwater gamma spectrometry: tank measurement results utilizing efficiencies calculated via Monte-Carlo code.

In order to study the sediment contamination, underwater gamma-ray spectrometry measurements performed by the sub-marine detector Canberra HpGe with a relative efficiency of 80% were carried out in an equipped tank at the Ukrainian Hydrometeorological Institute of Kiev. Different substrates, certified sources and experiment geometrical set-up were arranged. Efficiencies were calculated by in situ object counting system (ISOCS) software. ISOCS performance tests using certified sources were carried out by comparing laboratory measurements, and measurement results for mineralogy and density performances are reported.

Journal Article↗
PubMedDate not supplied

Lytic growth of human herpesvirus 8: morphological aspects.

The human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus, is a gamma herpesvirus associated with AIDS-related body cavity-based lymphomas (BCBL), also called primary effusion lymphomas (PEL). These are a rare form of non-Hodgkin lymphomas in which HHV-8 is present, often associated with Epstein-Barr virus (EBV) infection. HHV-8 is also present in a latent state or in a state of low-level persistence in different primary effusion lymphoma-derived cell lines, such BCBL-1 cells, that lack EBV infection. This cell line was induced to produce mature virions by treatment with 12-O-tetradecanoyl phorbol-13-acetate (TPA) and the characteristic ultrastructural features of HHV-8 lytic replication were identified and compared to those of the other members of Herpesviridae family.

Apoptosis↗
PubMedDate not supplied

Adeno-associated virus vectors for gene therapy of neurodegenerative disorders.

Adeno-associated virus (AAV) shows significant potential as a gene delivery system. Although it is ubiquitous in its distribution, with approximately 85% of the adult population in the United States seropositive for the virus, it has never been associated with clinical disease. Not only is AAV non-pathogenic, but it can infect with high efficiency both dividing and terminally-differentiated cells, moreover the wild-type virus integrates into a specific chromosomal site. It also has a broad host range and the virus is extremely resistant to environmental extremes. These characteristics make it particularly attractive as a gene delivery vehicle. As the enthusiasm driving the proliferation of clinical gene transfer protocols has dampened recently due to the lack of clinical success often reflecting immunogenicity and inefficiency of the gene transfer methods used in these trials, AAV with minimal (if any) toxicity and high efficiency in a wide range of cells and tissues, may become the vector-of-choice for many applications. There have been a number of comprehensive recent reviews of AAV biology and this article specifically discusses recent advances in the use of AAV vectors with particular emphasis on AAV vector-mediated in vivo gene transfer in the mammalian central nervous system.

Animals↗