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Biomedical subjects

P Lemaire

Publications and source records attributed to P Lemaire.

At least 19 recordsLinked to original sources
PubMed2002

Early steps in the formation of neural tissue in ascidian embryos.

Ascidians are simple invertebrate chordates whose lineage diverged from that of vertebrates at the base of the chordate tree. Their larvae display a typical chordate body plan, but are composed of a remarkably small number of cells. Ascidians develop with an invariant cell lineage, and their embryos can be easily experimentally manipulated during the cleavage stages. Their larval nervous system is organised in a similar way as in vertebrates but is composed of less than 130 neurones and around 230 glial cells. This remarkable simplicity offers an opportunity to understand, at the cellular and molecular levels, the ontogeny and function of each neural cell. Here, we first review the organisation of the ascidian nervous system and its lineage. We then focus on the current understanding of the processes of neural specification and patterning before and during gastrulation. We discuss these advances in the context of what is currently known in vertebrates.

Animals↗
PubMed2001

Do people combine the parity- and five-rule checking strategies in product verification?

The basic question of the present experiment was whether people use a combination of arithmetic problem solving strategies to reject false products to multiplication problems or whether they simply use the single most efficient strategy. People had to verify true and false, five and non-five arithmetic problems. Compared with no-rule violation problems, people were faster with (a) five problems that violated the five rule (i.e., N x 5 = number with 5 or 0 as the final digit; e.g., 15 x 4 = 62), (b) problems that violated the parity rule (i.e., to be true, a product must be even if either or both of its multipliers is even; otherwise, it must be odd; 4 x 38 = 149), and (c) problems that violated both the parity and five rules (e.g., 29 x 5 = 142). Finally, people were equally fast and accurate when they solved two-rule violation problems than when they solved five-rule violation problems, and faster for those two types of problems than for parity-rule violation problems. Clearly, people use the single most efficient strategy when they reject false product to multiplication problems. This result has implications for our understanding of strategy selection in both arithmetic in particular and human cognition in general.

Adolescent↗
PubMed2001

Induction of anterior neural fates in the ascidian Ciona intestinalis.

The sensory vesicle of ascidians is thought to be homologous to the vertebrate forebrain and midbrain (Development 125 (1998) 1113). Here we report the isolation of two sensory vesicle markers in the ascidian Ciona intestinalis, which are homologs of vertebrate otx and gsx homeobox genes. By using these markers to analyze the induction of anterior neural tissue in Ciona, we find that the restriction of anterior neural fate to the progeny of the anterior animal blastomeres is due to a combination of two factors. The vegetal blastomeres show a differential inducing activity along the anterior-posterior axis, while the competence to respond to this inducing signal is markedly higher in the anterior animal blastomeres than in the posterior animal blastomeres. This differential competence to respond is also observed in response to bFGF, a candidate neural inducer in ascidians (J. Physiol. 511.2 (1998) 347) and can be detected by the gastrula stage. Our results, however, indicate that bFGF can only induce a subset of the responses of the endogenous inducer, suggesting that additional signals in the embryo are necessary to induce a fully patterned nervous system.

Amino Acid Sequence↗
PubMed2001

The human chordin gene encodes several differentially expressed spliced variants with distinct BMP opposing activities.

During early embryogenesis of both vertebrates and invertebrates, antagonism between bone morphogenetic proteins (BMPs) and several unrelated secreted factors including Chordin (Chd) is a general mechanism by which the dorso-ventral axis is established. High affinity binding of Chd sequesters the BMP ligands in the extracellular space, preventing interactions with their membrane receptors. Another level of regulation consists in processing of vertebrate Chd or its Drosophila counterpart Sog by astacine metalloproteases like Xolloid-BMP-1/Tolloid, respectively, which releases an active BMP. Recently, it was shown that cleavage of Sog by Tolloid could generate novel BMP inhibitory activity and that sog is also capable of stimulation of BMP activity in a tolloid-dependant way. Activity and/or cleavage of Chd/Sog are influenced by other secreted factors like twisted gastrulation. In this study, we have cloned cDNAs of the human chordin gene (CHRD) and characterized alternative splice variants that code for C-truncated forms of the protein. We have found that CHRD is expressed in fetal as well as in adult tissues with relatively high levels in liver, cerebellum and female genital tract, suggesting functions in late embryogenesis and adult physiology. We also show that spliced variants are present with specific patterns in various tissues. When tested in an axis-duplication assay in Xenopus, we find that these variants can antagonize BMP activity. Altogether, these results suggest that, in addition to processing by metalloproteases, alternative splicing (AS) is another mechanism by which sub-products of CHRD can be generated to influence BMP activity in different developmental and physiological situations.

Alternative Splicing↗
PubMed2001

Siamois functions in the early blastula to induce Spemann's organiser.

In Xenopus, the Spemann organiser is defined as a dorsal territory in the early gastrula that initiates development of the embryonic axis. It has been shown that the early zygotic transcription factor Siamois is essential for Spemann's organiser formation. By the onset of gastrulation, the organiser is patterned into a vegetal head organiser, which induces anterior structures upon transplantation, and a more animal trunk organiser, which induces a posterior neuraxis. However, it is unclear when these distinct organiser domains are initially specified. To shed light on this question, we analysed the temporal activity of Siamois, as this factor induces both head and trunk development, when ectopically expressed via mRNA injection. In this study, we expressed Siamois ectopically at different time points and analysed the extent of axial development. Using a hormone-inducible version of Siamois, we found evidence for a tight window of competence during which ventral cells can respond to Siamois by commencing both the head and the trunk genetic programmes. The competence to form Spemann's organiser was lost 2 h before gastrulation, although partial axis formation could still occur following delayed activation of Siamois. We demonstrate that this late response to Siamois involves a new role for this gene, which can indirectly repress ventral gene expression, in the absence of known organiser genes.

Animals↗
PubMed2001

[Prenatal diagnosis of omphalopagus conjoined twins at 13 weeks of amenorrhea].

Conjoined twins are a rare occurrence. We present a case of conjoined twins at 13 weeks' gestation. This case demonstrated the possibility of making an accurate diagnosis of conjoined twins and delineating the extent of organ sharing in the first trimester. This analysis and the discover of another anomalies can help the parents with the option for pregnancy termination. Early diagnosis and precise delineation of the shared organs of conjoined twins are essential for optimal obstetric and postnatal management.

Female↗
PubMed2001

Older and younger adults' strategy use and execution in currency conversion tasks: insights from French franc to euro and euro to French franc conversions.

Younger and older adults were taught new strategies for converting amounts presented in French francs into euros or amounts presented in euros into French francs. The choice/no-choice method was used to obtain information on how often each newly learned strategy was used as well as information on the speed and accuracy of strategies. The results showed that both younger and older participants used the new conversion strategies unequally often and had strategy preferences that were justified by the relative ease of execution of each strategy. We discuss numerous practical applications of the present findings, as they suggest that one can help younger and older people by teaching them the add-half and divide-three strategies for the French-franc-euro conversions, that no specific strategies should be taught to older people, and that newly taught strategies are more efficient than those people use spontaneously.

Adolescent↗
PubMed2001

Phosphatidylinositol-3 kinase acts in parallel to the ERK MAP kinase in the FGF pathway during Xenopus mesoderm induction.

Phosphoinositide 3-kinases (PI3Ks) are lipid kinases that can phosphorylate phosphaditylinositides leading to the cell type-specific regulation of intracellular protein kinases. PI3Ks are involved in a wide variety of cellular events including mitogenic signalling, regulation of growth and survival, vesicular trafficking, and control of the cytoskeleton. Some of these enzymes also act downstream of receptor tyrosine kinases or G-protein-coupled receptors. Using two strategies to inhibit PI3K signalling in embryos, we have analysed the role of PI3Ks during early Xenopus development. We find that a class 1A PI3K catalytic activity is required for the definition of trunk mesoderm during the blastula stages, but is less important for endoderm and prechordal plate mesoderm induction or for organiser formation. It is required in the FGF signalling pathway downstream of Ras and in parallel to the extracellular signal-regulated kinase (ERK) MAP kinases. In addition, our results show that ERKs and PI3Ks can synergise to convert ectoderm into mesoderm. These data provide the first evidence that class 1 PI3Ks are required for a specific set of patterning events in vertebrate embryos. Furthermore, they bring new insight into the FGF signalling cascade in Xenopus.

Animals↗
PubMed2001

Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the notochord genetic programme in Xenopus gastrulae.

The Xenopus trunk organiser recruits neighbouring tissues into secondary trunk axial and paraxial structures and itself differentiates into notochord. The inductive properties of the trunk organiser are thought to be mediated by the secretion of bone morphogenetic protein (BMP) antagonists. Ectopic repression of BMP signals on the ventral side is sufficient to mimic the inductive properties of the trunk organiser. Resultant secondary trunks contain somite and neural tube, but no notochord. We show that inhibition of BMP signalling is sufficient for the initiation of the trunk organiser genetic programme at the onset of gastrulation. During late gastrulation, however, this programme is lost, due to an invasion of secreted Wnts from neighbouring tissues. Maintenance of this programme requires co-repression of BMP and Wnt signalling within the presumptive notochord region. To shed light on the molecular cascade that leads to the repression of the Wnt pathway, we looked for individual organiser genes whose overexpression could complement the inhibition of BMP signalling to promote notochord formation in the secondary trunks. Two genes, gsc and Xnot, were thus identified and shown to act in different ways. Xnot acts as a transcriptional repressor within the mesodermal region. Gsc acts in deeper vegetal cells, where it regulates Frzb expression to maintain Xnot expression in the neighbouring notochord territory. These results suggest that, during gastrulation, the necessary repression of Wnt/beta-catenin signalling in notochord precursors is achieved by the action of secreted inhibitors, such as Frzb, emitted by gsc-expressing dorsal vegetal cells.

Amino Acid Sequence↗
PubMed2001

Generation of the germ layers along the animal-vegetal axis in Xenopus laevis.

After completion of gastrulation, typical vertebrate embryos consist of three cell sheets, called germ layers. The outer layer, the ectoderm, which produces the cells of the epidermis and the nervous system; the inner layer, the endoderm, producing the lining of the digestive tube and its associated organs (pancreas, liver, lungs etc.) and the middle layer, the mesoderm, which gives rise to several organs (heart, kidney, gonads), connective tissues (bone, muscles, tendons, blood vessels), and blood cells. The formation of the germ layers is one of the earliest embryonic events to subdivide multicellular embryos into a few compartments. In Xenopus laevis, the spatial domains of three germ layers are largely separated along the animal-vegetal axis even before gastrulation; ectoderm in the animal pole region; mesoderm in the equatorial region and endoderm in the vegetal pole region. In this review, we summarise the recent advances in our understanding of the formation of the germ layers in Xenopus laevis.

Animals↗
PubMed2000

Influence of dietary carbohydrate on the metabolism of juvenile Litopenaeus stylirostris.

The effect of dietary carbohydrates (CBH) on glucose and glycogen, digestive enzymes, ammonia excretion and osmotic pressure and osmotic capacity of Litopenaeus stylirostris juveniles was studied. The increase of CBH, ranging between 1 and 33%, stimulates activities of alpha-amylase and alpha-glucosidase in the hepatopancreas. High levels of glucose in hemolymph and of glycogen in the hepatopancreas were reached at the highest level of dietary CBH; however, the kinetics of accumulation is different. Shrimps fed with low level of CBH needed 3 h to reached glucose peak, whereas only 1 h is necessary for high CBH levels. A saturation curve was observed in glycogen level and alpha-amylase activity with maximum values in shrimp-fed diets containing 21% CBH. This level could be used to be included as a maximum shrimp dietary CBH level. Pre-prandial glycogen levels were observed in shrimp fed a diet containing 1% CBH, indicating an important gluconeogenesis, which affected the protein metabolism. The present results show that a diet containing 10% CBH may not be enough to cover the CBH requirement, which could be satisfied by dietary protein content. The low osmotic capacity observed in shrimp fed on a diet containing 10% CBH coincided with a relatively low post-prandial nitrogen excretion which reflects a low concentration of amino acids circulating in hemolymph, which affected the osmotic pressure and the osmotic capacity. These results reflect the high plasticity of shrimp species to use protein to obtain metabolic energy from food and its limited capacity for processing dietary CBH.

Journal Article↗
PubMed2000

Children's strategy use in computational estimation.

This study reports an investigation of ten-year-old children's strategy use in computational estimation (i.e., give an approximate answer like 400 to an arithmetic problem like 224 + 213). Children used four strategies: rounding with decomposition, rounding without decomposition, truncation, and compensation. Strategies appeared to differ in frequency and effectiveness. Finally, children chose strategies in an adaptive way so as to obtain fast and accurate performance. Implications of these findings for understanding children's computational estimation performance and strategies in numerical cognition in general are discussed.

Child↗
PubMed1999

A two-step model for the fate determination of presumptive endodermal blastomeres in Xenopus embryos.

BACKGROUND: In Xenopus, the endoderm germ layer is derived from the vegetal blastomeres of cleavage-stage embryos. Cell transplantation experiments have revealed that the endodermal fate becomes gradually fixed during the late blastula stages. Sox17alpha, Mix.1, Mixer and GATA-4 encode vegetal zygotic transcription factors with endoderm-inducing activity. The accumulation of their transcripts during the late blastula stages may cause determination of the endodermal fate. VegT, a T-box transcription factor, the maternal transcripts of which are vegetally localised, is also required for endoderm formation. RESULTS: We analysed the events leading to the progressive accumulation of the transcripts for Sox17alpha, Mix.1, Mixer and GATA-4. Two phases could be distinguished in the endodermal programme. In phase 1, Sox17alpha, Mix.1, and the genes encoding transforming growth factor beta-related signalling molecules Xnr1, Xnr2 and Derrière were activated cell-autonomously at around the mid-blastula transition (MBT) by maternal determinants. In phase 2, TGFbeta signalling, possibly involving Xnr1, Xnr2 and Derrière, led to the activation of Mixer and GATA-4 in late blastula stages and to the reinforcement of the expression of Sox17alpha and Mix.1. Overexpression of VegT in animal caps triggered a developmental programme qualitatively similar to that observed in vegetal blastomeres, except that Xnr1 and GATA-4 were not activated by the early gastrula stage. CONCLUSIONS: Our results support a two-step model for endoderm determination between fertilisation and the onset of gastrulation. The initial cell-autonomous activation of early endodermal genes by maternal determinants including, but not limited to, VegT is relayed by the action of zygotic TGFbetas such as Xnr1, Xnr2 and Derrière.

Animals↗
PubMed1999

Solar wind outflow and the chromospheric magnetic network

Observations of outflow velocities in coronal holes (regions of open coronal magnetic field) have recently been obtained with the Solar and Heliospheric Observatory (SOHO) spacecraft. Velocity maps of Ne7+ from its bright resonance line at 770 angstroms, formed at the base of the corona, show a relationship between outflow velocity and chromospheric magnetic network structure, suggesting that the solar wind is rooted at its base to this structure, emanating from localized regions along boundaries and boundary intersections of magnetic network cells. This apparent relation to the chromospheric magnetic network and the relatively large outflow velocity signatures will improve understanding of the complex structure and dynamics at the base of the corona and the source region of the solar wind.

Journal Article↗
PubMed1999

Antagonist activity of DWnt-4 and wingless in the Drosophila embryonic ventral ectoderm and in heterologous Xenopus assays.

Wnt genes encode secreted signalling molecules involved in a number of basic developmental processes. In Drosophila, wingless and DWnt-4 are two physically clustered Wnt genes, which are transcribed in overlapping patterns during embryogenesis and, in several instances, are controlled by the same regulatory molecules. To address the question of the functional relationship of wingless and DWnt-4, we analysed how embryonic cells respond when they are exposed, simultaneously or not, to the encoded Wnt signals. We show that DWnt-4 has the capacity to antagonise Wingless signalling both in the Drosophila ventral epidermis and in a heterologous system, the Xenopus embryo. We provide evidence that DWnt-4 inhibits the Wingless/Wnt-1 signalling pathway upstream of the activation of transcriptional targets. This is the first report that antagonising Wnt signals exist in Drosophila.

Animals↗
PubMed1999

What effects strategy selection in arithmetic? The example of parity and five effects on product verification.

The parity effect in arithmetic problem verification tasks refers to faster and more accurate judgments for false equations when the odd/even status of the proposed answer mismatches that of the correct answer. In two experiments, we examined whether the proportion of incorrect answers that violated parity or the number of even operands in the problem affected the magnitude of these effects. Experiment 1 showed larger parity effects for problems with two even operands and larger parity effects during the second half of the experiment. Experiment 2 replicated the results of Experiment 1 and varied the proportion of problems violating parity. Larger parity effects were obtained when more of the false problems violated parity. Moreover, all three effects combined to show the greatest parity effects in conditions with a high proportion of parity violations in problems containing two even operands that were solved during the second half of the experiment. Experiment 3 generalized the findings to the case of five rule (i.e., checking whether a false product ends in 5 or 0), another procedure for solving and verifying multiplication problems quickly. These results (1) delineate further constraints for inclusion in models of arithmetic processing when thinking about how people select among verification strategies, (2) show combined effects of variables that traditionally have been shown to have separate effects on people's strategy selection, and (3) are consistent with a view of strategy selection that suggests a bias either in the allocation of cognitive resources in the execution of strategies or in the order of execution of these strategies; they argue against a simple, unbiased competition among strategies.

Adolescent↗
PubMed1998

Developmental signalling: a careful balancing act.

Embryos of arthropods and chordates are patterned along the dorso-ventral axis by a gradient of secreted morphogens of the Bmp4/Dpp family. This gradient now appears to be shaped by the opposing activities of Bmp-sequestering proteins, on the one hand, and Bmp-releasing metalloproteases, on the other.

Animals↗