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Biomedical subjects

P Legmann

Publications and source records attributed to P Legmann.

89 records · Page 5Linked to original sources
PubMed1984

[Ischemic accidents of the lower limbs with preservation of the peripheral pulse after arteriography (3 cases )].

Marked ischemia of the lower limbs and abdomen, with conservation of peripheral pulses, was noted several hours after arteriography in 3 patients. The origin of the lesion was due to migration of microemboli (cholesterol crystals) in one patient, but was conjectural in the other two cases. Possible etiologies include a chemotoxic effect of the contrast medium used, as well as the action of other factors (sterilization of materials), and the arteriographic technique applied (reflux technique in two cases).

Angiography↗
PubMed1978

[Polysulfates, anionic detergents, sodium phosphotungstate and electrophoretic mobility of plasmatic proteins].

Anions with strong electro-negative charges, like sulphuric esters of polysaccharides (heparin, dextran sulphate M.W. 15 00), the sodium salt of polyanethol sulfonic acid (liquoid, Roche), anionic detergents (sodium dodecylsulphate, sodium oleate, sodium deoxycholate), and the sodium salt of phosphotungstic acid were added to human serum or citrated plasma. For each compound several final concentrations were adopted, the highest being 4%. By using microimmunoelectrophoresis and numerous specific antisera against human plasma proteins, it was demonstrated that at pH 8.60 anions increase electrophoretic mobility of the following antigens: lipoproteins alpha and beta; fibrinogen; beta 2-glycoprotein I; beta 2-glycoprotein II ; antithrombin III. All reagents utilized do not react with all these proteins; for instance, only detergents accelerate the migration rate of a lipoproteins. Besides, depending on the protein, this or that reagent may be the most active. Thus, in the polysulphate group, heparin has the highest affinity for antithrombin III, liquoid for fibrinogen and dextran sulphate for beta 2-glycoprotein I.

Blood↗
PubMed1976

[Detergents and precipitation of serum lipoproteins by antisera (author's transl)].

By using gel diffusion methods, it was shown that during immunoelectrophoresis and double diffusion in two dimensions, anionic detergents (sodium oleate, sodium desoxycholate, sodium lauryl-sulfate and sodium decyl-sulfate), added to human serum at the final concentration of 0.5%, do not prevent the formation of an insoluble antigen-antibody complex (precipitation line) when specific antibodies react with serum lipoproteins: LDL, HDL and VLDL. In spite of the presence of anionic detergents, the antigen-antibody interaction in gel occurs even if the amount of lipoproteins in serum was highly reduced. On the contrary, the non ionic detergent tween 80, has a strong inhibitory effect on the specific precipitation of lipoproteins, especially in the case of HDL.

Anions↗
PubMed1976

Purification of rabbit anti-human fibrinogen antibodies cross reactivity of mammalian fibrinogen.

A simple isolation procedure of highly purified and unaltered rabbit antibodies to human fibrinogen is described. The method used is based upon the preferential adsorption of fibrinogen by barium sulfate at acid pH. After redissolution of the specific precipitate and dissociation of the antigen-antibody complexes at pH 2.4, the fibrinogen is removed by adsorption on barium sulfate, while the antibody protein stays in solution. BaSO4 is eliminated by centrifugation and the supernatant is neutralized and concentrated by ammonium sulfate fractionation (40% saturation). A concentrated solution of specific anti-fibrinogen antibodies is thus obtained; the latter can be used to prepare, by selective immunprecipitation, a fibrinogen free plasma, and to analyze precipitin patterns in cross reacting systems.

Animals↗
PubMedDate not supplied

Peliosis hepatis: triphasic helical CT and dynamic MRI findings.

We report the triphasic helical computed tomographic (CT) and dynamic magnetic resonance imaging (MRI) findings in a unique case of biopsy-proven peliosis of the liver. Several reports have described the CT and MRI findings of this entity without specific appearance. This report discusses the usefulness of dynamic helical CT and MRI for the early diagnosis of peliosis.

Female↗
PubMedDate not supplied

Hepatofugal portal flow in advanced liver cirrhosis with spontaneous portosystemic shunts: effects on parenchymal hepatic enhancement at dual-phase helical CT.

BACKGROUND: We investigated whether spontaneous hepatofugal portal flow in advanced cirrhosis affects hepatic enhancement on dual-phase helical computed tomography. METHODS: Fifteen patients with hepatofugal portal flow on Doppler sonography and angiography (group 1) and 15 age- and sex-matched patients with hepatopetal portal flow (control; group 2) underwent dual-phase helical computed tomography. Vascular and liver attenuation values were measured on unenhanced scans and scans obtained during hepatic arterial (HAP; 25 s) and portal venous (PVP; 70 s) phases. RESULTS: Portal vein enhancement was lower during PVP in group 1 than in group 2 ( p < 0.0001). Liver enhancement was higher during HAP and lower and delayed during PVP in group 1 versus group 2 ( p < 0.0001). CONCLUSION: In hepatofugal portal flow, liver enhancement is increased during HAP and compensating for decreased and delayed liver enhancement during PVP, resulting in potential decreased hypervascular tumor conspicuity.

Collateral Circulation↗
PubMedDate not supplied

CT of fibrolamellar hepatocellular carcinoma.

We retrospectively evaluated 10 patients with pathologically proven fibrolamellar hepatocellular carcinoma (FLHCC) to assess the utility of CT (n = 9), ultrasound (n = 10), angiography (n = 9), and MR (n = 3). The tumors were solitary in 6 of 10 patients, well delineated in 7 of 10, and hypervascular in 8 of 10. With use of multimodality imaging techniques, a central scar was demonstrated in 3 of 10 patients, calcifications in 4 of 10, dilated intrahepatic ducts in 2 of 10, thrombosis of a segmental portal branch in 2 of 10, and lymph node involvement in 3 of 10. Computed tomography was the most accurate technique for diagnosis and staging. Magnetic resonance imaging helped establish the correct diagnosis in one patient. This report emphasizes the wide variability of imaging features of FLHCC and the predominant role of CT for suggesting the diagnosis.

Adolescent↗
PubMedDate not supplied

Hyperacute rejection in liver transplantation: CT findings.

Hyperacute rejection is rare after liver transplantation. We report a case of early graft failure following liver transplantation in a 46-year-old woman. On the 5th postoperative day, progressive loss of graft function occurred. Liver histology showed submassive hepatic necrosis. Computed tomography features showing large geographic areas of necrosis without anatomic distribution were suggestive of hyperacute rejection.

Female↗
PubMedDate not supplied

Detection of myocardial involvement in patients with sarcoidosis applying T2-weighted, contrast-enhanced, and cine magnetic resonance imaging: initial results of a prospective study.

PURPOSE: To assess myocardial magnetic resonance imaging (MRI) findings in sarcoidosis. METHODS: Cardiac assessment was prospectively performed in patients with sarcoidosis and included physical examination, electrocardiogram, echocardiography, thallium-201 myocardial scintigraphy, and coronary angiography if coronary disease was suspected. T2-weighted black-blood single-shot and inversion recovery fast spin echo, functional gradient echo, and T1-weighted gadolinium-DTPA-enhanced cardiac MRI examinations were performed in 40 patients while other cardiac disease was excluded. RESULTS: Results of the MRI images were normal in cardiac-asymptomatic stage I or Lofgren syndrome patients (n = 4). Among the patients with cardiac-asymptomatic multiple organ sarcoidosis, 17 of 31 patients (54%) had MRI myocardial abnormalities similar to those observed in five patients with cardiac symptoms: nodular peripheral increased intramyocardial signal intensity on both T2-weighted and contrast-enhanced images (n = 5), focal or patchy increased signal on contrast-enhanced images with or without myocardial thickening (n = 10), and focal increased signal only on T2-weighted images with or without myocardial thinning (n = 2). Focal contractility abnormalities were noticed in nine patients. CONCLUSIONS: These preliminary results emphasize the occurrence of subclinical myocardial MRI abnormalities in patients with ongoing systemic sarcoidosis.

Adult↗
PubMedDate not supplied

[Guided punctures].

Explore the source record for details and available documents.

Biopsy, Needle↗
PubMedDate not supplied

[Specific rabbit abtiserum against human chylomicrons and very low density lipoproteins].

Rabbits were immunized by repeated intravenous injections of large amounts of chylomicrons plus VLDL isolated from human lipemic plasmas. The antisera were absorbed usccessively with lipoprotein free serum, HDL and LDL2. The absorbed antisera still precipitate chylomicrons and VLDL from sera of normal and hyperlipemic subjects (post-absorptive donors and fasting patients with type IV and type V hyperlipoproteinemia). By gel diffusion methods (immunoelectrophoresis and double diffusion in two dimensions), the antisera reveal a single precipitin line which stains better for lipid than for protein. This line is pronounced in VLDL-rich sera and absent in VLDL-free sera. The antisera react with chylomicrons plus VLDL delipidated by extraction with ethanol/diethyl ether; this shows that the antigenic site is an apoprotein; in contrast to apoprotein C, this apoprotein appears to be unique to the triglyceride-rich lipoproteins.

Animals↗
PubMedDate not supplied

[Weber-Christian syndrome and pancreatic involvement. Apropos of a case with review of the literature].

The Weber-Christian syndrome associates generalized nodular inflammatory panniculitis with osteoarticular manifestations. Pathogenesis of this rare affection is still obscure but a pancreatic origin has been reported in the literature. A case is presented having the particular features of being revealed by a subacute episode of chronic pancreatitis, and by the intensity of radiologic bony manifestations.

Adult↗