Biomedical subjects
P L Ratner
Publications and source records attributed to P L Ratner.
Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries.
Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.
Genetic regulation of the constitutive D-ribose operon in Escherichia coli B/r.
Merodiploid complementation analysis of the constitutive synthesis of the D-ribokinase and the D-ribose permease in Escherichia coli B/r has shown that the constitutive D-ribose operon is genetically controlled by a transdominant regulatory gene closely linked to the D-ribokinase and D-ribose permease structural genes. The regulatory mechanism for this operon shows no requirement for operator-repressor interaction, rather a truly positive control mechanism and thus suggests an extension of the operon model in its application to constitutive enzyme regulation in bacteria.