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Biomedical subjects

P Krogh

Publications and source records attributed to P Krogh.

48 records · Page 3Linked to original sources
PubMed1975

Detection of ochratoxin A in barley, using silica gel minicolumns.

A simplified procedure has been developed to detect ochratoxin A in cereals which can be used in the field where equipment for thin layer chromatography is not available. The procedure includes extraction of the acidified sample with chloroform, purification over sodium bicarbonate, and minicolumn chromatography. Under longwave ultraviolet light ochratoxin A appears as a blue-green fluorescent band at the lower end of the column. Contamination levels as low as 12 ppb can be detected by this method.

Chromatography, Thin Layer↗
PubMed1974

Fate of ochratoxin A and citrinin during malting and brewing experiments.

The fate of ochratoxin A and citrinin during malting and brewing processes was studied by the use of naturally contaminated lots of barley, as well as by the addition of crystalline toxins to the mash. Complete degradation was observed for ochratoxin A from moderately contaminated barley lots and for citrinin added to mash. The use of highly contaminated barley resulted in transmission of ochratoxin A into the beer, but only 2 to 7% of the initial content was detected, corresponding to levels of 6 to 20 mug of ochratoxin A per liter of beer. Barley lots with this high ochratoxin contamination (1,000 to 5,000 mug/kg) will be easily detected and, therefore, because of pronounced deterioration, should be rejected during inspection upon admittance to the breweries.

Amylases↗
PubMedDate not supplied

Time-dependent disappearance of ochratoxin A residues in tissues of bacon pigs.

Crystalline ochratoxin A was administered to bacon pigs for one month. After termination of toxin exposure the pigs were slaughtered at different intervals and analyses for ochratoxin A residues in four tissues were conducted. Kidneys contained the highest concentrations, and fat the lowest, at each interval. Ochratoxin A disappeared from muscles and fat after 2 weeks, from liver after 3 weeks, and from the kidneys after 4 weeks. The toxin disappeared from tissues exponentially. All the pigs would have passed the meat inspection because no pathologic lesions were developed although tissues contained mycotoxin residues. The results of this study indicate that contamination of meat by ochratoxin A may be avoided by feeding pigs ochratoxin-free feed during the last 4 weeks before slaughter.

Animals↗
PubMedDate not supplied

Reye's syndrome in a child on long-term salicylate medication.

A case of Reye's syndrome in a four-year-old child on long-term salicylate medication for rheumatoid arthritis is reported. Severe fatty changes of the liver, lipid vacuolation in the renal proximal tubules, and severe brain oedema were the prominent postmortem findings. Symptoms of a trivial infection and vomiting just before death added to the resemblance of this case to Reye's syndrome.

Arthritis, Juvenile↗
PubMedDate not supplied

Human exposure to ochratoxin A in areas of Yugoslavia with endemic nephropathy.

Ochratoxin A is a mycotoxin with pronounced nephrotoxic potency in all species of single-stomach animals studied; it is a major disease determinant of porcine nephropathy and a disease occurring endemically in several countries. This disease is comparable with Balkan (endemic) nephropathy, suggesting a common causal relationship. Ochratoxin A has been found in foodstuffs in many countries, but the highest frequency of ochratoxin A contamination in foods (10.3% of 1,553 samples of foodstuffs) was encountered in an area of Yugoslavia, where Balkan (endemic) nephropathy is prevalent. Detection of ochratoxin A in human blood samples confirmed the prevalent exposure to this food contaminant. Relative risk calculations indicated a tendency to an association between this mycotoxin and Balkan (endemic) nephropathy, supporting the hypothesis of a causal role of ochratoxin A in this disease.

Balkan Nephropathy↗
PubMedDate not supplied

Measurements of paralytic shellfish poisons. A review of biological and chemical procedures.

Paralytic shellfish poisons (PSP) are derivatives of a tetrahydropurine base, which includes saxitoxin, 1-hydroxy saxitoxin, and 11-hydroxy saxitoxin sulfate. These compounds are produced by Gonyaulax catenella and other dinoflagellate species, and the algal toxins (phycotoxins) contaminate shellfish during the filter-feeding process performed by the bivalve molluscs. Increased incidence of algal blooms and outbreaks of PSP food poisonings have been encountered in recent years, resulting in a demand for closely monitoring of shellfish for these toxins. The existing biological and chemical procedures for analysis are reviewed. The mouse bioassay is commonly used in routine analysis, but is nonspecific. Several of the chemical procedures are specific and more sensitive than the mouse bioassay. The disadvantage, however, seems to be that not all known PSP compounds can be quantitiatively measured by any single chemical procedure.

Animals↗