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Biomedical subjects

P Kraus

Publications and source records attributed to P Kraus.

At least 73 records · Page 4Linked to original sources
PubMed1976

Biodegradation of alpha-hexachlorocyclohexane. VII. Resolution, purification, and characterization of an alpha-HCH dechlorinating enzyme from rat liver cytosol.

From rat liver cytosol, an enzyme was isolated which catalyzes the dechlorination of alpha-, gamma-, and delta-HCH. The enzyme also catalyzes the conjugation of GSH with 1,2-dichloro-4-nitrobenzene, and with 1 -chloro-2,4-dinitrobenzene. The enzyme has a molecular weight of about 46000 and its molecule is composed of two subunits of similar size. The optimum for the dechlorination of alpha-HCH lies at pH 8.0. The Michaelis constant is 0.12 mM for alpha-HCH (with 1 mM GSH as constant substrate), and maximal velocity was determined to be 0.25 moles Cl- -min -1 per mole enzyme.

Animals↗
PubMed1975

Biodegradation of alpha-hexachlorocyclohexane. VI. The cechlorination of alpha-hexachlorocyclohexane by microsomes and cytosol of rat liver.

Alpha-hexachlorocyclohexane (alpha-HCH) is dechlorinated by enzymes contained in rat liver cytosol and microsomes. An evidence was obtained that in the cytosol there are two alpha-HCH dechlorinating enzymes at least; one operates only in the presence of reduced glutathione (GSH) and catalyzes dechlorinations associated with the formation of another hydrophilic product. This product is probably a conjugate of the alpha-HCH-residue with GSH. The other cytoplasmic alpha-HCH-dechlorinase requires no additions. The microsomes, too, contain two alpha-HC dechlorinases at least: one is stimulated by GSH, the other by NADPH.

Acetone↗