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Biomedical subjects

P Kemppinen

Publications and source records attributed to P Kemppinen.

25 records · Page 2Linked to original sources
PubMed1989

Immunological studies on patients with optic neuritis without evidence of multiple sclerosis.

We studied 6 patients with idiopathic optic neuritis (ON) after a mean follow-up period of 12 years. No evidence of multiple sclerosis (MS) was found on clinical grounds, nor by magnetic resonance imaging (MRI) or evoked responses. Levels of spontaneous proliferation, serum gamma-interferon (IFN-gamma) and lymphocyte MHC locus II antigen were similar in ON and in 6 patients with progressive MS, but different in healthy controls. This suggests that similar immunological conditions in vivo prevail after an idiopathic ON and in MS. A normal PHA-induced interleukin-2 receptor expression and lymphocyte proliferation but a low IFN-gamma secretion and MHC locus II antigen expression were observed. This suggests that an ON and MS defect is expressed after an interleukin-2 receptor ligand but before IFN-gamma secretion. Our findings also suggest that immunological factors do not explain the neuropathological confinement of the lesions in ON but the protective influence may rather be exerted by some as yet undefined genetic influence or may be explained by the different etiology of ON and MS.

Adult↗
PubMed1989

Localisation of lysozyme mRNA in rheumatoid synovial membrane by in situ hybridisation.

Type A synovial lining cells have been shown to contain lysozyme in their lysosomes. This might be phagocytosed because synovial fluid contains lysozyme originating from tissue macrophages and articular cartilage but in arthritides, in particular, from neutrophils. In situ hybridisation with 35S labelled cDNA was used to detect mRNA for lysozyme over synovial lining in patients with rheumatoid arthritis. No hybridisation was found with lactoferrin cDNA, which was used as a negative control. Computer search against the EMBL gene bank (release 14) did not show any significant cross hybridisation to a known sequence. In cytological specimens 35S-cDNA:mRNA hybrids were observed in positive but not in negative control cells. The presence of lysozyme and its mRNA suggests that type A synovial lining cells are of mononuclear phagocyte lineage.

Arthritis, Rheumatoid↗
PubMed1989

PHA stimulation of peripheral blood lymphocytes in oral lichen planus. Abnormality localized between interleukin-2 receptor ligand formation and gamma-interferon secretion.

Peripheral blood T cell function in five oral lichen planus (OLP) patients and five healthy controls was assessed using different activation parameters. Staining with monoclonal antibodies against interleukin-2 receptor and MHC locus II coded Ia antigen, 3H-thymidine incorporation and gamma-interferon secretion were determined in phytohaemagglutinin (PHA) stimulated peripheral blood mononuclear cell cultures at days 0, 1, 3 and 5. The peripheral blood T cell subsets and spontaneous MHC locus II antigen expression were similar in OLP patients and in controls whereas the spontaneous lymphocyte proliferation was lower in OLP patients than in controls (p less than 0.01). This may reflect a slight in vivo preactivation and its effect on lymphocyte recirculation. The PHA-induced expression of IL-2 receptor and T cell proliferation were similar in both groups whereas gamma-interferon secretion and MHC locus II antigen expression were low in OLP patients compared with controls (p less than 0.01). The results suggest a defect in OLP T cell activation disclosed by in vitro PHA stimulation and localized between IL-2 receptor ligand binding and gamma-interferon secretion.

HLA-D Antigens↗
PubMed1988

Corneal endothelial cell density after trabeculoplasty.

Central corneal endothelial photographs were taken with a specular microscope. The cell density was calculated with a computerized image analyzer. No difference between the treated and control eyes could be found. The laser energy level delivered in trabeculoplasty seems to be too small to induce any permanent changes in the endothelial cells.

Aged↗
PubMed1988

Synovial fibroblasts.

Chronic synovitis refers to fibrin deposits on the surface of proliferating superficial synovial lining cells leading to villous hyperthrophy, and associated with foci of cell necrosis and infiltration of chronic inflammatory cells. The superficial synovial lining cells include fibroblast-like type B cells and macrophage-like type A cells. Fibroblast- and macrophage-like cells in the early or leading edge of pannus may represent an extension of these cells, which themselves may be nothing else than stromal fibroblasts and macrophages adapted to the particular micro-milieu prevailing at the interface of hyaluronate (HA) containing synovial fluid and richly vascularized loose connective tissue in the sublining stroma. In all three locations fibroblasts are exposed to various humoral substances, extracellular matrix (ECM) and cell-cell contacts, which may modify their phenotype and function. Therefore, one would expect differences in the fibroblasts in inflammatory and non-inflammatory synovial tissue. These changes can be best understood by considering some of the basic fibroblast properties, namely migration, substrate adherence, proliferation and synthesis and degradation of ECM.

Animals↗