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Biomedical subjects

P Kaufmann

Publications and source records attributed to P Kaufmann.

At least 199 records · Page 11Linked to original sources
PubMed1977

[The ultrastructure of Langhans cells in pathologic human placentas (author's transl)].

In electron microscopic observations on the villous cytotrophoblast in pathologic human placentas we find, besides a general increase of the amount of Langhans cells, significant ultrastructural differences according to the different diseases in pregnancy. Diabetes mellitus: the most striking observations are small, extremely electron dense mitochondria and a lot of intracellular filaments in a hydropic cytoplasm. Rh-incompatibility: according to the stage of placental damage the Langhans cells show a different structure. In light cases we find hydrolic cells with normal mitochondria as well as swollen ones. The plasmalemm shows a lot of foldings and dentations with the syncytium. In serious cases of rh-incompatibility the Langhans cells cover the trophoblastic basal membrane completely. Their electron density is even higher than that of the syncytium. Characteristic lysosomes appear. In the most serious cases the syncytium is completely destroyed and maximal hydropic Langhans cells cover the villi. EPH-gestosis: the Langhans cells show different phases of differentiation, but no other characteristic criteria. The importance of the Langhans cells for the regeneration of the syncytotrophoblast is discussed. Mitochondria and rough endoplasmic reticulum are obviously not formed in the syncytium but they must be regenerated by taking up Langhans cells by syncytial fusion.

Blood Group Incompatibility↗
PubMed1977

[Problems of distinction of normal, arteficial and pathological structures in mature human placental villi. I. Ultrastructure of the syncytiotrophoblast (author's transl)].

Thirty-four mature human placentae were punctured in situ during cesarean section and fixed immediately. The ten cases which seemed normal according to clinical and morphological criteria were examined electron-microscopically. Findings won in ideal conditions after perfusion fixation of the guinea-pig placenta were compared to the human punction samples. The granular endoplasmic reticulum in the syncytium proved to be the most susceptable parameter. Its cisternae are narrow and arranged parallel to each other after optimal fixation. The larger the ischemic phase--already detectable after 2 min--, the more disorientated and dilated they become. The mitochondria only react considerably later. Similar structural alterations also occur in the course of pathological processes. The meaning of arteficially and pathologically induced ultrastructural changes and their interdependance are discussed.

Animals↗
PubMed1977

The villous stroma of the human placenta. I. The ultrastructure of fixed connective tissue cells.

In human placental villi the connective tissue is constructed by mesenchymal cells, small and large reticulum cells and fibroblasts. During early pregnancy mesenchymal cells dominate; starting with the third month of gestation the reticulum cells are in the majority within the terminal villi, the fibroblasts within the stem villi. Ultrastructurally intermediary types of cells can be differentiated. Together with reticular and collagenous fibres the reticulum cells form the basic architecture of the villous stroma during the first 2/3 of gestation: the "reticular type of stroma". This consists of a network of cells and fibres with fetal vessels fitted in between. The remaining interspaces form a fluid system of compartments in which Hofbauer cells are suspended. They are called stromal channels. During the last trimester these channels and the Hofbauer cells as well are progressively replaced either by voluminous masses of fibres ("fibrous type of stroma", mainly in the stem villi) or by sinusoidal enlargements of fetal capillaries ("sinusoidal type of stroma", mainly in the terminal villi).

Cell Differentiation↗
PubMed1977

Multiple family therapy: a new direction in the treatment of drug abusers.

Multiple family therapy is presented as an important treatment augmentation for drug abusers in a variety of settings. The total family is viewed as patient with the drug abuser a symptom bearer. Common family dynamics are described as well as the techniques to create structural changes in families.

Ambulatory Care↗
PubMed1976

Reactions to iophendylate in relation to multiple sclerosis.

A retrospective survey was made of 57 consecutive patients referred for myelography by one neurological team. Reactions occurred in patients with multiple sclerosis significantly more often than in all other patients (P=0.01). This finding appears to support the belief that demyelination may be associated with more severe reactions to iophendylate myelography.

Adult↗
PubMed1976

[The effect of 2,4-dinitrophenol, monoiodine acetate, sodium fluoride and hypoxia on the formation of plasma polyps in the placenta of guinea pigs (author's transl)].

Plasma polyps can be found at the end of a normal pregnancy. Following NaF and MJA-intoxication, there is an extreme increase of plasma polyps. This accelerated formation can be prevented by the injection of Na-pyruvate and often the number of polyps can be reduced below the norm. 2,4-Dinitrophenol intoxication and breathing of hypoxic gas mixture (2,5% O2, 5% CO2, 92,5% N2) do not lead to increased plasma polyp formation. This proves, that it is not hypoxia, not an energy deficit secondary to separation of oxydative phosphorylation and not cardiac insufficiency leading to secondary ischemia which are responsible for the extreme increase in the number of plasma polyps following the injection of MJA and NaF.

Acetates↗
PubMed1975

Experiments on infarct genesis caused by blockage of carbohydrate metabolism in guinea pig placentae.

The poisoning of pregnant guinea pigs with monoiodineacetate, an inhibitor of carbohydrate metabolism in the glyceraldehyde-3-phosphate dehydrogenase phase, leads within a few minutes to the formation of syncytial plasma protrusions in the maternal blood lacunae of the placenta. These protusions cause a primary white infarct where the degenerative process takes place--in the interlobium. An accumulation of erythrocytes originates in its arterial inflow area owing to the obstruction of the blood flow. Both primary infarct and accumulation of erythrocytes alter to form a single homogenous white infarct within a few days, together with the degenerative products of all cellular and syncytial elements. Timely substitution of glycolysis for pyruvate, i.e. within 20 min, during continued blockage of the glyceraldehyde-3-phosphate dehydrogenase, prevents the formation of an infarct.

Animals↗
PubMed1975

Adsorption of DNA molecules to different support films.

Protein-free adsorption of the DNA of the Escherichia coli bacteriophage T7 to carbon, collodion, aluminium-beryllium and aluminium films was studied. It was found that the appearance of DNA strands depended greatly upon the kind of support film used. Direct adsorption of DNA to aluminium-beryllium or aluminium films yielded specimens with 'thin and long' and 'thick and short' regions along the strand. Well extended, uncoiled and unaggregated DNA molecules were obtained only when DNA was adsorbed to carbon, collodion or mica in the presence of intercalating dyes such as ethidium bromide. Adsorption properties of the different films are well correlated with their surface charge. Aluminium-beryllium films carry a strong positive surface charge, aluminium films a weak positive charge and carbon films a weak negative charge. It is suggested that for the preparation of specimens by spontaneous adsorption of well extended and unaggregated strands it is necessary that the DNA molecule is stiffened by a ligand such as an intercalating dye, and that the charge on the surface of the support film is opposite to the charge of the macromolecule.

Adsorption↗
PubMed1975

[Migrant eruptions in lupus erythematosus. Apropos of a case of lupus erythematosis gyratus repens].

Rare cases of migrant circinated eruptions occurring during the course of lupus erythematosus have been published so far. They have been classified in two categories: 1) supposed toxicodermias due to antimalarials; 2) real cases of erythema annulare centrifugum, mostly inaugurating the L. E. In an exceptional case, a migrating eruption looking like an erythema annulare with a complicated pattern on the trunk, neck and limbs, took place between two rashes of typical L.E. The exact nature of this eruption, clinically and histologically atypical, and its relation with the L.E. itself, have been discussed.

Aged↗