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Biomedical subjects

P Jullien

Publications and source records attributed to P Jullien.

At least 55 records · Page 3Linked to original sources
PubMed1974

Interferon synthesis in x-irradiated animals v. Origin of mouse serum interferon induced by polyinosinic-polycytidylic Acid and encephalomyocarditis virus.

The radioresistant cell systems producing serum interferon after intravenous administration of polyinosinic-polycytidylic acid [poly(I.C)] or encephalomyocarditis virus in mice were studied in rat-to-mouse radiation chimeras. Interferon induced by poly(I.C) became of donor type within 3 months after grafting of irradiated C3H/He mice with Wistar rat bone marrow cells; this indicated that it was made in cells derived from the hemopoietic system. In contrast, encephalomyocarditis virus-induced interferon remained of recipient type in xenogeneic chimeras up to 3 months after grafting, which indicated that the bulk of this interferon originated from a cell population not derived from the hemopoietic system. To ascertain that the respective radiosensitivities of the systems producing rat interferon in chimeras corresponded to that of normal mice, some rat-to-mouse chimeras were subjected to a second X irradiation 1 month after the first irradiation and restoration. Circulating interferon production was studied 4 days later. As expected, the re-irradiation strongly depressed rat serum interferon production induced by Newcastle disease virus but had no effect on rat interferon synthesis induced by poly (I.C). These results point to a macrophage origin for the bulk of poly(I.C)-induced circulating interferon.

Journal Article↗
PubMed1969

Interferon synthesis in x-irradiated animals. IV. Donor-type serum interferons in rat-to-mouse radiation chimeras injected with Newcastle disease virus.

C(3)H/He mice were exposed to total-body X-irradiation of 1000 roentgens and received thereafter 10(7) xenogeneic Wistar rat marrow cells intravenously. In these rat-to-mouse chimeras, serum interferon-producing capacity upon injection of Newcastle disease virus was examined four weeks after grafting. Normal levels of circulating interferon were produced. The interferon, however, had the species specificity of rat interferon, being 20 times more active in rat embryo fibroblasts than in mouse embryo fibroblasts. Moreover, it behaved like rat interferon when filtered on Sephadex G-100 dextran gel, displaying one peak of activity in the 90,000 molecular-weight range and two incompletely separated peaks of 32,000 and 24,000, respectively, in the 30,000 molecular-weight region. The fact that the interferon is of the donor type in rat-to-mouse chimeras permits us to conclude that circulating interferon induced by New-castle disease virus is made in bone-marrow-derived cells.

Animals↗