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Biomedical subjects

P J Naish

Publications and source records attributed to P J Naish.

3 recordsLinked to original sources

Variation in cephalometric reference lines.

The use of intracephalic reference lines in cephalometric assessment is valid only if these lines bear some constant relationship to the true horizontal and to each other. Otherwise, the use of a true horizontal would give the benefit that cephalometric assessment would be based on the same standards as clinical judgment. In using a true horizontal, however, the radiograph must be taken with the head in a reproducible natural posture. A study was carried out to assess the reproducibility of a natural head posture using a simple positioning method in a standard cephalostat, and to assess the variation in the relationship of the sella-nasion, Frankfort, maxillary and mandibular reference lines, to a true horizontal and to each other. Though in their mean inclinations the Frankfort and the maxillary reference lines were almost parallel to each other and to the true horizontal, the range of variation for all the reference lines was considerable. The range of variation in the reference lines was greater than the error of reproduction of head position, and the range of variation of the slope of the mandibular line was no greater than that for the slope of each of the other three lines. The sella-nasion line was reproduced more accurately that the other reference lines, using a digitizer. The possibilities of a simple cephalometric analysis based on a true horizontal are outlined.

Adolescent↗

Rapid assay for theophylline in clinical samples by reversed-phase high-performance liquid chromatography.

A fast, sensitive and highly specific method for the determination of theophylline in human serum is reported. Using a C18-bonded reversed-phase column with an acetonitrile-acetate buffer mobile phase theophylline is completely resolved not only from other dietary xanthines and their metabolites but also from co-administered drugs such as paracetamol and phenobarbitone. Use of beta-hydroxyethyltheophylline as internal standard allows a within batch precision of 2.0% and a between batch variation of 3.0%. Factors involved in the development of the method and its performance are discussed.

Asthma↗

Theophylline estimation-a comparative evaluation of a gas chromatographic method and a high-performance liquid chromatographic method.

A comparative evaluation of a gas-liquid chromatographic (GLC) assay and a high-performance liquid chromatographic (HPLC) assay for the determination of plasma theophylline levels has been carried out. Both methods are reliable, accurate, and technically simple and appear to be specific for theophylline. The HPLC system is more sensitive (sample volume 25 micrliter) than the GLC system (sample volume 500 microliter) and is therefore suitable for the estimation of theophylline in microsamples. The HPLC system also has better precision (coefficient of variation: within-batch 2.0%, between-batch 3.0%) than the GLC system (coefficient of variation: within-batch 3.5%, between-batch 5.6%). Good correlation (r=0.964) was found between plasma theophylline levels (n=75, observed range 4-186 mumol/l) determined by each procedure. The results of this study indicate that, of the two systems investigated, HPLC is the method of choice.

Chromatography, Gas↗