Visual and tactile assessment of neuromuscular blockade.
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Anesthesia↗
Biomedical subjects
P Hung
Publications and source records attributed to P Hung.
Cyclic AMP regulation of lactate dehydrogenase. Quantitation of lactate dehydrogenase M-subunit messenger RNA in isoproterenol-and N6,O2'-dibutyryl cyclic AMP-stimulated rat C6 glioma cells by hybridization analysis using a cloned cDNA probe.
We have cloned DNA complementary to mRNA coding for rat C6 glioma cell lactate dehydrogenase M-subunit. Double-stranded DNA complementary to a portion of lactate dehydrogenase mRNA was inserted into the Pst I site of plasmid pBR322 by the dC.dG tailing technique and amplified in Escherichia coli HB101. A recombinant plasmid containing lactate dehydrogenase cDNA was identified by colony hybridization to a cDNA prepared from partially purified lactate dehydrogenase mRNA and by hybridization-selected translation. The recombinant plasmid (pRLD42) contains a 680 nucleotide insert of lactate dehydrogenase mRNA. Hybridization of nick-translation pRLD42 to glioma cell poly(A)+RNA separated on agarose gel and transferred to nitrocellulose exhibited Mr = 5.9 X 10(5) for lactate dehydrogenase mRNA. Furthermore, Northern blot analysis of RNA from unstimulated and isoproterenol-stimulated glioma cells indicated a 2-fold increase of lactate dehydrogenase mRNA molecules in stimulated cells. The 2-fold increase of lactate dehydrogenase mRNA was confirmed by RNA-excess kinetic hybridization using pRLD42 DNA and poly(A)+RNA from unstimulated, isoproterenol-, and dibutyryl cAMP-stimulated glioma cells. These data demonstrate that isoproterenol and dibutyryl cAMP cause an increase of the number of lactate dehydrogenase M-subunit mRNA molecules in glioma cells which, in part, determines the extent of synthesis of the lactate dehydrogenase M-subunit.
Animals↗
Nurse education. Continuous assessment.
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Education, Nursing↗
Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA.
Hybrid plasmid molecules containing tandemly repeated Drosophila satellite DNA were constructed using a modification of the (dA)-(dT) homopolymer procedure of Lobban and Kaiser (1973). Recombinant plasmids recovered after transformation of recA bacteria contained 10% of the amount of satellite DNA present in the transforming molecules. The cloned plasmids were not homogenous in size. Recombinant plasmids isolated from a single colony contained populations of circular molecules which varied both in the length of the satellite region and in the poly(dA)-(dt) regions linking satellite and vector. While subcloning reduced the heterogeneity of these plasmid populations, continued cell growth caused further variations in the size of the repeated regions. Two different simple sequence satellites of Drosophila melanogaster (1.672 and 1.705 g/cm3) were unstable in both recA and recBC hosts and in both pSC101 and pCR1 vectors. We propose that this recA-independent instability of tandemly repeated sequences is due to unequal intramolecular recombination events in replicating DNA molecules, a mechanism analogous to sister chromatid exchange in eucaryotes.
DNA↗
Transfer RNA activities of Rous sarcoma and Rous associated viruses.
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Amino Acids↗
Proteins of avian tumor viruses with different coat antigens.
Isoelectric focusing of avian tumor viruses with distinct type-specific envelope antigens demonstrated no differences in isoelectric points. Viruses with different type-specific antigens were found to contain different glycoprotein components when virion proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Acrylates↗
Preoperative fasting of patients undergoing elective surgery.
Preoperative fasting regimens tend to be based in ritualistic practice causing physical and psychological harm to patients. This research study investigates current nursing practice and concludes that it must be research based.
Clinical Protocols↗
Pre-operative fasting.
This descriptive research study replicates that of Hamilton-Smith and investigates the current practice of pre-operative fasting. It was found that, 20 years on, the management of pre-operative fasting was still largely unchanged. Despite the recognition of vast potential complications of prolonged fasting, most patients were deprived of food and fluid for considerable lengths of time before general anaesthesia--far beyond the acceptable maximum fasting time of 12 hours. However, a small number of experienced nurses challenged the tradition and successfully reduced pre-operative fasting near to the accepted minimum and thereby provided adequate nutrition for patients.
Adult↗