Biomedical subjects

P Huber

Publications and source records attributed to P Huber.

309 records · Page 18Linked to original sources

PubMedDate not supplied

Hormone dependency and the action of tamoxifen in human mammary carcinoma cells.

Conditions are described which allow to demonstrate estradiol dependency of proliferation in MCF-7 human mammary carcinoma cells in a reproducible manner. Our results concerning the action of Tamoxifen on the growth of MCF-7 cells differ in two aspects from previous reports: (a) The inhibitory effect of pharmacological doses (10(-7) M) is only obvious if the cells are stimulated by estradiol. (b) In the absence of estradiol, Tamoxifen acts as a weak estrogenic agonist, by stimulating the growth of MCF-7 cells. This finding could imply that premenopausal women should not be treated with Tamoxifen after ovariectomy.

Breast Neoplasms↗

PubMedDate not supplied

[Applications of polymerase chain reaction in identifying the D1S80 (pMCT 118) locus in forensic DNA analysis].

The value of the amplified fragment length polymorphism at the D1S80 locus was examples by means of examples of forensic casework (paternity and identification cases). Parameters, like PCR conditions, limit of detection and analysis of mixed samples are discussed. In a population study the allele and genotype frequencies of 154 individuals from the area of Bonn were determined. The distribution of genotypes was in agreement with expected values according to the Hardy-Weinberg equilibrium. There was no deviation from other published Middle European studies.

Alleles↗

PubMedDate not supplied

[Fetal fibronectin as a marker of prematurity in a high risk patient sample].

The accuracy of cervicovaginal fetal fibronectin as a predictor of preterm birth was studied in patients with increased risk for preterm delivery (according to the Creasy-score). In a prospective blind observational study the smear from the posterior fornix vaginae of 56 pregnant patients without PROM was examined using a quantitative immunoassay for the detection of fetal fibronectin. The patients who tested positively for fetal fibronectin had significantly more preterm deliveries than those with a negative result (CHI square-test, p < 0.01, RR 5.1). Overall, sensitivity, specificity, positive and negative predictive values were 56%, 87%, 45% and 91%, respectively. In patients with preterm labor these values were 75%, 87%, 60%, and 93%, respectively. No patient with a negative result delivered preterm during the following two weeks. It is concluded that performing the fetal fibronectin test in patients with preterm labor is useful for the prediction of preterm birth. Routine testing in patients at increased risk (asymptomatic patients) is not recommended for lack of effectiveness.

Female↗