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Biomedical subjects

P Holmstrup

Publications and source records attributed to P Holmstrup.

At least 91 records · Page 5Linked to original sources
PubMed1982

Effect of Saliment on parotid salivary gland secretion and on xerostomia caused by Sjögren's syndrome.

The stimulatory effect of a recently introduced saliva substitute (Saliment) on the parotid salivary secretion was experimentally investigated in 10 healthy individuals without signs or symptoms of xerostomia and in 11 patients with xerostomia due to Sjögren's syndrome. Further, in 15 patients with Sjögren's syndrome, the effect of Saliment on the sensation of xerostomia was examined in a double-blind clinical study with cross-over. The use of Saliment was followed by a significant stimulation in the parotid salivary secretion (P less than 0.01) and the subjection sensation of xerostomia was significantly reduced (P less than 0.01).

Adolescent↗
PubMed1981

Deposits of immunoglobulins, complement, and fibrinogen in oral lupus erythematosus, lichen planus, and leukoplakia.

Direct immunofluorescent staining (IF) for detection of deposits of IgG, IgM, IgA, complement C3, and fibrinogen at the basement membrane zone has been performed on a total of 279 biopsy specimens from oral lesions and clinically normal oral mucosa from the following groups of patients: fourty-five discoid lupus erythematosus (DLE), including sixteen patients without skin lesions, seven systemic lupus erythematosus (SLE); fourty-five lichen planus (LP); thirty leukoplakia (Leuk.); twenty patients with uncertain diagnosis, termed DLE? LP?; and twenty-three healthy persons. Deposits of immunoglobulins (lg) occurred in 73 percent of oral lesions in DLE and in 100 percent of the oral lesions in SLE, whereas lg deposits occurred in 3 to 30 percent in the other groups, the differences being significant (P less than 0.05). It is concluded that lg deposits are a characteristic feature of oral lesions of DLE and SLE and only rarely occur in oral lesions of LP and Leuk., which are the most important differential diagnoses. A direct immunofluorescence test on oral lesions suspected to be LE should be regarded as positive only if lg deposits are present, since C3 occurs frequently in oral lesions other than LE. Consideration can be given to the inclusion of lg deposits in the diagnostic criteria for oral LE lesions.

Adolescent↗
PubMed1981

A method for studying cholesteatomas in vivo, with special attention to epithelial-mesenchymal interaction.

A model for studying human cholesteatomas in vivo in the immunodeficient 'nude' mouse is described. Transplanted cholesteatoma membrane from 13 patients showed in 10 marked and fast growth in 14-21 days, whereas 3 showed necrosis. findings of cystic lesions lined by a thin stratified keratinizing epithelium were frequent and in contrast with transplanted jaw keratocyst the connective tissue reaction was weak. This experimental system may be useful for further studies of factors of importance for the regulation of differentiation and growth of middle ear mucosal epithelium.

Animals↗
PubMed1981

Human buccal mucosa transplants in nude mice.

The development of a model to study tissue interactions in human oral mucosa in vivo utilizing transplantation of human tissue to nude mice is described. Ninety-six samples of normal human buccal mucosa were transplanted to subcutaneous sites in the flank region of nude mice and were protected from overlying cutaneous and subcutaneous tissue by polyethylene capsules or by Millipore filters. Transplant recovery rates were 47% an 69%, respectively. The histologic features of the epithelium of transplanted specimens were compared to 20 biopsies of normal buccal mucosa. The epithelium of transplants maintained for 3 weeks or longer with either type of protection, maintained features essentially similar to the normal controls but was somewhat thinner.

Animals↗
PubMed1981

Oral leukoplakia transplanted to nude mice.

Thirty-eight biopsies of homogeneous oral leukoplakias were transplanted to subcutaneous sites of nude mice and protected from overlying cutaneous tissue by Millipore filters. The transplants were recovered after 21 d with a rate of successful recovery of 66%. The histologic epithelial features of the transplants were compared with those of the original leukoplakias. None of the leukoplakias from patients without tobacco habits lost the preexisting keratinization, whereas all 10 transplants demonstrating loss of keratinization belonged to patients with tobacco habits. This difference in behavior of transplanted leukoplakias from patients with and from patients without tobacco habits was statistically significant (P = 0.028). The results of the experimental study support the concept of two different types of leukoplakia, one possessing irreversible changes in epithelial keratinization, the other showing reversibility.

Adult↗
PubMed1981

Epithelial outgrowths from human buccal mucosa transplants in nude mice.

The histologic features of epithelial outgrowths from transplants of human buccal mucosa in nude mice are described. Nine-six samples of normal buccal mucosa were transplanted to subcutaneous sites in the flank region of nude mice. The tissue specimens were maintained in the mice for periods ranging from 1 to 67 d and were protected from overlying murine cutaneous and subcutaneous tissue by polyethylene capsules or by Millipore filters. The epithelial outgrowth from the transplants were formed over murine connective tissue, with and without substantial infiltrate of neutrophils, and over Millipore filter with and without interjacent murine connective tissue. The stratified squamous epithelium was essentially unkeratinized and thinner than that overlying originally transplanted connective tissue. The histologic features of the outgrowths were similar irrespective of the technique used or the type of underlying tissue.

Animals↗
PubMed1981

Normal keratinized mucosa transplants in nude mice.

Two types of normal keratinized mucosa were transplanted to subcutaneous sites of nude mice of two different strains. 24 intact specimens of clinically normal human palatal mucosa were transplanted to nude mice of the strain nu/nu NC. The transplants were recovered after 42 d with a recovery rate of 96%. Moreover, 22 intact specimens of normal rat forestomach mucosa were transplanted to nude mice of the strain nu/nu BALB/c/BOM. These transplants were recovered after 21 d with a recovery rate of 63%. The histologic features of the transplants were essentially the same as those of the original tissues. However, epithelial outgrowths from the transplants differed with respect to the pattern of keratinization. The outgrowths of human palatal mucosa transplants were essentially unkeratinized, while the outgrowths of the rat forestomach transplants showed continued keratinization.

Adolescent↗
PubMed1979

Changes in carbohydrate expression of lichen planus affected oral epithelial cell membranes.

The purpose of the present study was to examine possible epithelial cell membrane changes in lichen planus affected oral mucosa by the use of the lectins concanavalin-A and ricinus communis agglutinin fraction I. It was shown that these lectins in contrast to what was found in normal epithelium do not bind to the cell membranes of basal cells of lichen planus affected epithelium. The lack of binding of lectins suggests changes in exposed carbohydrates at the surfaces of the cells. The findings may support the hypothesis of antigenic changes in the lichen planus affected epithelium.

Cell Membrane↗
PubMed1979

Erythroplakic lesions in relation to oral lichen planus.

In a cohort of 740 referred patients with oral lichen planus eight patients were found with a sharply demarcated slightly depressed erythroplakic area. The histological examination of the biopsies from the erythroplakic lesion of the 8 patients showed seven with epithelial dysplasia. In addition, two of the lesions revealed a squamous cell carcinoma. In the erythroplakic lesion without dysplasia the histological picture was found to be characteristic of lichen planus. All eight patients were seen at later follow-up examinations (median observation period: 3.6 years) and in one patient a squamous cell carcinoma arose in the erythroplakic lesion during the control period. It is concluded that the reported erythroplakic lesions in relation to oral lichen planus seem to be premalignant and they should be followed and/or treated like oral erythroplakias.

Aged↗
PubMed1977

Smoking habits of 611 patients with oral lichen planus.

The smoking habits of 611 patients (68 per cent females and 32 per cent males) with oral lichen planus were studied. Forty-six per cent were daily smokers, 4 per cent smoked only at social events, and 50 per cent were nonsmokers. In comparison with the nonsmokers, the daily smokers showed significantly lower prevalences of reticular and atrophic types of oral lichen planus lesions and a significantly higher prevelance of the plaque type (P less than 0.001). It is suggested that these findings depend on a mechanism whereby original atrophic and reticular types of lesions are altered into the plaque type of lesions under the influence of smoking. The question arises whether such plaque type of lesion can be regarded as leukoplakias which have been superimposed on the oral mucosa affected by lichen planus.

Adolescent↗
PubMed1977

Cellular hypersensitivity to oral lichen planus lesions in vitro.

The purpose of the present investigation was to demonstrate in vitro cellular hypersensitivity to oral lichen planus lesions. Twenty oral lichen planus patients and 20 control persons matched by and sex were examined by the leukocyte migration agarose technique with tissue extracts from oral lichen planus lesions, normal oral mucosa and normal human kidney, the two latter extracts serving as control antigens. The female lichen planus patients showed statistically significant (P=0.004) migration inhibition with extracts from oral lichen planus lesions, which supports the hypothesis of cellular hypersensitivity to oral lichen planus lesions. However, the male lichen planus patients did not show any significant reaction. No significant reaction was found with the control antigens. The findings do not exclude the hypothesis of cellular hypersensitivity to oral lichen planus lesions. Furthermore, they do not support the hypothesis of oral lichen planus being an autoimmune disease.

Adult↗
PubMed1977

Arterial blood pressure in patients with oral lichen planus.

The arterial blood pressure of 121 patients with oral lichen planus was determined using the auscultatory method. These values were then compared with those previously found in a general adult population. No significant difference was found betwween the values of the lichen planus patients and those of the general population. The present investigation does not support the hypothesis of a relationship between lichen planus and hypertension.

Adult↗
PubMed1977

Glucose tolerance in patients with oral lichen planus.

Of 123 patients with oral lichen planus two were known diabetics. The remainder were tested for fasting glucosuria, 113 for fasting blood glucose and 103 also with an oral glucose tolerance test. According to the criteria of WHO, 18 of the 123 patients were diabetics. In most of these patients the oral glucose tolerance test was only slightly abnormal. No decrease of the glucose tolerance with increasing duration of lichen planus was demonstrable. Comparison with two general population studies did not reveal any significant difference between the glucose tolerance of the lichen planus patients and that of the general population samples.

Adult↗