Diffusional effects on the heterogeneous kinetics of two-substrate enzymic reactions.
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Aspartate Aminotransferases↗
Biomedical subjects
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Diffusional effects on two-substrate enzymic reactions mainly depend on the relative affinities of the enzyme for its two substrates. With two substrates of widely different affinities, diffusional limitations increase and decrease the half-maximal-activity concentration of the high-and low-affinity substrate respectively.