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Biomedical subjects

P Herve

Publications and source records attributed to P Herve.

142 records · Page 8Linked to original sources
PubMed1975

[Application of free flow preparative methods for the separation of blood cells].

Human blood cells can be separated using an apparatus described by Hanning and co-workers (Model FF4 Desaga Heidelberg). The method of free flow preparative electrophoresis uses a specific property of blood cells when subjected to an electric field: their electrophoretic mobility. Three regions of migration can be defined: --a high mobility region (HMR); --a low mobility region (LMR) and --an intermediate mobility region (IMR). After the electrophoretic migration, the various cell fractions are identified. If human leucocytes are subjected to an electric field (40 volts/cm) it is possible to isolate several populations which differ by their membranes electric charge. The repartition of the neutrophiles granulocytes is twofold: --one fraction of high mobility (HMR) and --one fraction of low mobility (LMR). The meaning of these two populations is being discussed. Between these teo fractions is located the lymphocyte fraction which is represented by a single peak of intermediate mobility (IMR). Eosinophiles and monocytes are concentrated in low mobility fraction (LMR). With appropriate migration parameters (80 volts/cm, various component buffers) it is possible to obtain from lymphocytes, purified by density gradient, an electrophoretic separation of T and B lymphocytes population. Histograms obtained from quantitative measures (on collected cell fractions) show an unimodal distribution. When separated cells are characterized by three membranes markers (E. Rosettes, EAC Rosettes and surface immunoglobulins) the electrophoretic heterogeneity of B and T cells is then demonstrated. With E Rosettes (T cells) the majority of T lymphocytes is distributed in HMR and IMR. With EAC Rosettes and membrane immunofluorescence (B cells markers) the majority of B lymphocytes is found in the LMR. Thus an important enrichment of T and B sub-population is obtained, but contamination from one another still remains too important. More selective methods should lead to an improvement of this cell separation.

B-Lymphocytes↗
PubMedDate not supplied

Human platelets frozen with glycerol in liquid nitrogen: biological and clinical aspects.

Platelets were frozen using glycerol (3% in plasma) as a cryoprotective agent, a rapid cooling rate, and liquid nitrogen for storage. The cryopreserved platelets were thawed at 42 C and infused without washing. The results indicate that the quality of the thawed platelets is equivalent to platelets stored for 24 to 48 hours at room temperature. The availability of HLA phenotyped leukocyte poor platelets can reduce the frequency of sensitization to strong antigens and provide clinically effective platelets for alloimmunized patients.

Blood Platelets↗
PubMedDate not supplied

[Low prevalence of ant-hepatitis c virus antibodies in hepatocellular carcinoma in Senegal].

Senegal is a country with a high incidence of hepatocellular carcinoma (HCC). The role of the hepatitis B virus (HBV) in the genesis of HCC is well established. To evaluate the role of the hepatitis C virus (HCV) in the occurrence of HCC in Senegal, second-generation serologic tests were used to compare the prevalence of anti-HCV antibody in subjects with HCC and in controls without liver disease. A polymerase chain reaction assay was used to look for HCV RNA in sera from patients with HCC and anti-HCV antibody. HBV markers were assayed in all study subjects. Prevalences of anti-HCV antibody were 10.9% (7/64) in the HCC group and 5% (3/58) in the control group (NS). In the HCC group, anti-HCV antibody was found in 2.4% (1/42) of subjects positive for the hepatitis B antigen (HBsAg) versus 27.2% (6/22) of subjects negative for the HBsAg (p < 0.01). HCV RNA was detected in five of six anti-HCV-positive HCC patients. Prevalence of the HBsAg was 65.6% (42/64) in the HCC group versus 17.2% (10/58) in the control group (p < 10-8). Anti-HBV antibody was detected in 48.3% (28/58) of controls versus 6.3% (4/64) of HCC patients (p < 10-8). These data do not support a close epidemiologic association between the HCV and HCC in Senegal, where the HBV remains the main viral cause of HCC.

Adolescent↗