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P Heilmann

Publications and source records attributed to P Heilmann.

At least 73 records · Page 4Linked to original sources
PubMed1983

[Superficial veins of the neck].

Course, width, and mouth of the Vv. jugularis externa, jugularis anterior, suprascapularis, transversa colli, vertebralis anterior, and retromandibularis are estimated and the variability of the arcus venosus juguli is included.

Adult↗
PubMed1981

[Problems in using radioactively labeled bacteria in animal experiments. 4. Distribution of activities in the mouse following intratracheal administration of labeled Pasteurella multocida].

Intratracheal administration of 3H-labelled, 14C-labelled, 59Fe-labelled or 125J-Labelled Pasteurella multicida germs to mice resulted in more or less differentiated, nuclide-dependent, distributions of radioactivity in blood, spleen, liver, lung, kidney, and gastro-intestinal tract. All distributions were comparable to those following subcutaneous application. Elimination of antigen from lungs and other organs could be characterised by an e-function, once a certain level of distribution had been reached. Some of the antigen was persistent in the lung not less than 14 days. Extremely high activity concentration and persistence was recordable, following the use of 59Fe complete antigen. Phagocytosis of Pasteurella multicida germs through alveolar macrophages of the lung was secured by autoradiography. Most of the antigen seemed to be discharged from the lungs through the digestive tract. Antigen distributions recorded from immunised and non-immunised mice seemed to suggest that the fate of antigen applied was affected by the kind of immunisation. No difference in antigen distribution between non-immunised and subcutaneously immunised animals were recordable, following intratracheal antigen application, but is was clearly recordable, following intratracheal immunisation. Elimination of antigen from the lungs of intratracheally immunised animals was found to occur faster than it did from non-immunised animals.

Animals↗
PubMed1980

[Studies of diarrhea in young calves. 3. Effect of cyclophosphamide on histomorphology and microbial flora of the gastrointestinal tract in the calf].

One single intravenous injection of 60 mg/kg of cyclophosphamide caused the development of moderate to medium catarrhal enteritis in calves, within two to four days from injection. --Distally aggravating damage to epithelial cells in the small intestine, accompanied by shortening of villi, as well as usually complete lymphocyte depletion of the lymph follicles of Peyer's patches were the most conspicuous pathohistological findings obtained from the intestinal tract. Regular epithelial damage, including ulcerations and necroses, were recordable also from the colonic mucous membrane. --No remarkable change in the gastro-intestinal flora was recordable from calves killed two days from cyclophosphamide application, but the quantitative composition of the gastro-intestinal germ flora was found to be remarkably altered in those animals which had been killed four days after administration of the cytostatic. --Elevated coli counts in the rumen as well as in the proximal and distal parts of the jejunum and ileum and reduced anaerobic overall germ counts and bacteroides values in the cecum and rectum were the most conspicuous bacteriological findings. --The direct damaging action of cyclophosphamide on the mucous membrane as well as altered immunological reactivity of the experimental animals should be considered the triggering factors which actually caused considerable displacement in the quantitative composition of the gastro-intestinal flora in those calves which had been killed four days after cyclophosphamide application.

Animals↗
PubMed1977

[Demonstration of antibody forming cells in various calf organs].

Reported in this paper are studies relating primarily to methodical aspects of qualitative and quantitative identification of all classes of immunoglobulin-forming cells by means of fluorescent antibody in different tissues of calf. Particular attention was given to the respiratory tract. The method enables to study the postnatal development of the local secretory defence system in the context of all immunoglobulin classes.

Animals↗
PubMed1977

[Problems in the use of radioactively labelled bacteria in experiments. 2. Quantitative method for the evaluation of pathogens in the calf lung by means of labelled bacteria after aerosol or intratracheal administration].

By means of two aerosol apparatures radioactive labelled Pasteurella-multocida-germs were given to calves via a respiratory mask. Labelling of Pasteurellae was done internal by using special nutritive media with Fe-59 or P-32. In a period of 10-25 minutes 5 X 10(9)-10(10) labelled bacteria were given to the animals for inhalation. Another animal group was injected intratrachealy with the same quantity of labelled germs. Immediately after termination of germ application the animals were killed and dissected. After solution or homogenization, respectively of the total lung tissue radioactivity was determined by liquid-scintillation-counting (Fe-59) or Cerenkov-measurement (P-32), respectively. The activities recovered from lung homogenates rendered is possible to calculate the received amount of bacteria. 40-80% of germs would be recovered in the lung after application by intratracheal injection while only 1-7% could be recovered after individual aerosol application lasting for 15-25 minutes.

Aerosols↗
PubMed1977

[The reaction of calves to parenteral administration of cyclopheosphamide].

One single injection of 50 mg/kg live weight cyclophosphamide or more to calves was followed by a latency of few days after which pneumonia or enteritis developed and caused death within one or two weeks. Cyclophosphamide application led to pronounced changes in the white blood count characterised, at the beginning, by rise of neutrophilic granulocytes and decline of lymphocates. Primary rise of granulocytes then was followed by almost complete disappearance of them. In those calves that survived the parameters of the white blood count were not restoredto normal until several weeks had elapsed. While an injection of 30 mg/kgcyclophosphamide usually was tolerated without any visible clinical reaction, it was also accompanied by the above pronounced changes in the white blood count. The activity of the reticulohistiocytary system, as recordable by means of ink and bacterial clearance, was not markedly affected by one single injection of 30mg/kg or 40 mg/kg cyclophosphamide. Calves with cyclophosphamide treatment exhibited unambigous humoral immune response, yet somewhat delayed or reduced in comparison to controls. While one single intravenous injectionof 30 mg/kg cyclophosphamide alone failed to trigger any clinical disease, it proved to be capable of rendering calves more susceptible to pneumonia pathogens. It, therefore, might be suitable for supporting experimental infection and thus facilitating the study of enzootic pneumonia of calf which usually is difficult to reproduce.

Animals↗
PubMed1976

[General adaptation syndrome (Selye) in the calf. 5. Effect of increased glucocorticosteroid values on the phagocytosis activity of luekocytes, the function of RHS and the morphology of lymphatic organs].

Studies were conducted with the view to elucidating the correlations between increased glucocorticosteroid levels in the blood and the defense potential of calf organism against infectious diseases. The test animals were exposed to several substances (ACTH, cortisol, colibacteria, coliendotoxin), and even one to two days of increased 11-OHKS values were followed by marked decline in phagocytosis activity of leucocytes. In addition, RHS function was considerably reduced, after ten to thirteen days of application had elapsed, since at that point the disappearance of intravenously applied bacteria from circulating blood of test animals took place at rates which were much lower than those recorded from untreated calves. Differentiated length of stress or action (four to thirteen days) was followed by conspicuous changes in the lymphatic tissue of calf organism, with severe involution of thymus and follicular atrophy of intestine-associated lymphatic tissue having been the major findings. The results seem to suggest that rise in adrenocortical hormone level under stress may reduce potential organic defense to infection.

11-Hydroxycorticosteroids↗
PubMed1975

[Concentration and subcellular distribution of iron and copper in the pig liver. II. Studies on the livers of porkers and swine for slaughter with regard to the addition of copper sulfate to the diet].

In the livers of weaners, an average concentration of storage iron of 69.1 +/- 47.6 mug, and in those of pigs for slaughter, 326 +/- 54 mug/g tissue was measured. Addition of 250 ppm of copper as CuSO4-5H2O to the animals' food led to a significantly lower concentration of 248 +/- 63-5 mg Fe/g liver in pigs for slaughter. Of the storage iron, 66-1% for weaners, 79.2% for pigs for slaughter, and 76% for animals given Cu-supplemented food were localized in the microsomal fraction and in the cytosol. The copper concentration in the liver was 5.2 +/- 0.6 mug/g in weaners, 8.1 +/- 2.4 mug/g in pigs for slaughter and 120 +/- 83 mug/g in animals kept on a Cu-supplemented diet, Of these, 72.6% (pigs for slaughter) and 71.9% (weaners) came to the microsomal fraction and the cytosol, whereas addition of 250 ppm copper to the food ration enhanced the proportion in the nuclear fraction from 7.7 to 29.5%, and in the mitochondrial fraction, from 19.8 to 45.1%. The percent distribution of the copper on the subcellular fractions depends on the copper concentration in the liver: in the range of 5-12 mug/g tissue, increased copper concentration in the liver caused preferential localization of the copper in the cytosol, whereas between 25 and 330 mug/g tissue the copper was stored subcellularly in the following order: mitochondrial, nuclear, cytoplasmatic and microsomal fractions.

Animals↗