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Biomedical subjects

P He

Publications and source records attributed to P He.

At least 55 records · Page 3Linked to original sources
PubMed2001

Mechanism and kinetics of low-temperature thermochemical conversion process for sewage sludge.

The low-temperature thermo-chemical conversion process for sewage sludge is a prospective technology, through which the energy in the sludge can be recovered. With the help of elementary analysis of sewage sludge and its conversion products, thermal gravimetric analysis (TGA) of the sludge and GC/MS analysis of the derived oil, a study was carried out on element transfer, characteristic conversion temperature and conversion reaction mechanism of the process. The following results are obtained: 1) the predominant conversion reactions are distillation of aliphatic compounds, splitting of protein peptide bonds and group transfer; and 2) the main components involved in the conversion are aliphatic compounds and protein, with the lower reaction temperature for the former, the higher for the latter and the highest for saccharides. Based on the mechanism analyses, the simplified reaction model of the thermo-chemical conversion process for sewage sludge consists of two serial competitive reactions (producing volatile matter and char respectively). The estimated Arrhennius kinetic parameters of the reaction model based on TGA testing resultsare A1 = 4.15 x 10(6) 1/s, n1 = 2, E1 = 98 kJ/mol; A2 = 1.42 x 10(5) 1/s, n2 = 2, E2 = 85 kJ/mol; A3 = 1.01 x 10(12) 1/s, n3 = 4, E3 = 190 kJ/mol; A4 = 1.33 x 10(9) 1/s, n4 = 4, E4 = 146 kJ/mol.

Conservation of Natural Resources↗
PubMed2001

[Study on expression of caspase-3 and bcl-2 proteins and their relations with cell apoptosis and proliferation in non-Hodgkin's lymphoma].

OBJECTIVE: To explore the possible roles of caspase-3 and bcl-2 proteins in the development and progression of non-Hodgkin's lymphoma (NHL) and their relationship. METHODS: TdT-mediated dUTP nick end labeling (TUNEL) and immunohistochemistry were used to study cell apoptosis and expression of proliferating cell nuclear antigen (PCNA), caspase-3 and bcl-2 in 119 NHL cases. RESULTS: The caspase-3 and bcl-2 positive rates were 86.6% (103/119) and 53.8% (64/119) respectively. Contrasting expression of these two proteins were found in reactive and malignant lymphoid tissues: In reactive lymphoid follicles, caspases-3 exhibited strong immunoactivity in cells located in germinal centers but not in mantle zone lymphocytes, whereas bcl-2 exhibited strong immunoactivity in mantle zone lymphocytes but not in cells located in germinal centers, and in neoplastic follicles, caspase-3 was negatively or weakly expressed, whereas bcl-2 was often positively expressed. In B cell lymphomas, the high grade group had a higher caspase-3 immunointensity and a lower bcl-2 immunointensity than the low grade group (P < 0.01). Apoptotic index correlated positively with expression of caspase-3 (r = 0.512, P < 0.01) and inversely with expression of bcl-2 (r = -0.436, P < 0.01). In addition, apoptotic index and proliferative index were positively related (r = 0.710, P < 0.01). CONCLUSIONS: caspase-3 may participate in the regulation mechanism of lymphoma cell apoptosis. The contrasting expression of caspase-3 and bcl-2 is often seen in reactive and malignant lymphoid tissues, and it may indicate a close relationship between these two proteins in the regulation of lymphocyte proliferation kinetics.

Adolescent↗
PubMed2001

[Cloning and expression of VHB gene in D-arabitol producing yeast].

Recombinant plasmid pVgb-EX2 containing Vitreoscilla hemoglobin gene vgb and formaldehyde resistant gene SFA1 was constructed and transformed into D-arabitol producing yeast strain Saccharomyces sp. X-62. The fact that the amount of VHb in transformant cells was considerably higher than that in control cells indicated that gene vgb was expressed in transformant cells. D-arabitol productivity and yield of fermentation by transformants were improved. The most improvement of D-arabitol productivity in repeat experiments reached 27.3%. It appeared that the fermentation productivity of D-arabitol was relative to the amount of VHb in cells under experimental conditions.

Bacterial Proteins↗
PubMed2000

Human glioma cell BT325 expresses a proteinase that converts human plasminogen to kringle 1-5-containing fragments.

Angiostatin, a specific angiogenesis inhibitor, is an internal fragment of plasminogen, and can be generated in many systems mediated by different enzymes in vitro. The mechanism of angiostatin generation in vivo has not been well defined. Here we demonstrated that human glioma cell line BT325 can express an enzyme that can convert purified plasminogen to angiostatin-like fragments with molecular masses of 65, 60, and 58 kDa, respectively. These fragments have an identical N-terminal as KVYLS, which starts from Lys(98) of the plasminogen precusor. According to their molecular mass, the three fragments should comprise kringle domain 1 to kringle domain 5 (kringle 1-5). The proteolytic fragments obtained as above can inhibit the growth of bovine aortic endothelial (BAE) cells specifically. The proteolysis process can be completely inhibited by serine proteinase inhibitors, and partially inhibited by EDTA. The molecular weight of the peptide, which contains an enzymatic activity responsible for the proteolysis, was 13 kD determined by gel filtration and SDS-PAGE. The present data suggest that glioma cell BT325 can produce a novel proteinase to generate kringle 1-5 of plasminogen as an angiogenesis inhibitor.

Amino Acid Sequence↗
PubMed2000

On-line coupling of subcritical water extraction with high-performance liquid chromatography via solid-phase trapping.

Although ambient water is very polar and cannot dissolve many organic species, water at elevated temperatures behaves like a polar organic solvent. Thus, subcritical water has been proven to be an effective extraction fluid for several classes of organic compounds. While solvent trapping was used to collect the extracted analytes in most of previous subcritical water extractions, sorbent trapping has also been developed for subcritical water extraction. In this study, an on-line system for subcritical water extraction and high-performance liquid chromatography (HPLC) was built and tested. A sorbent trap was used as the interface between subcritical water extraction and HPLC. Several shut-off valves have been utilized to switch the system from one mode to another (e.g., from the extraction mode to HPLC mode). The coupling technique of subcritical water extraction and HPLC eliminates the liquid-liquid extraction used in solvent trapping subcritical water extraction and provides higher sensitivity. Compared to the off-line system reported in an earlier work, the operation of this on-line system is even easier. Some peak broadening occurred after the coupling the water extraction with HPLC for the analytes studied. The performance of this on-line system was evaluated by the extraction and determination of caffeine, nitrotoluenes, polychlorinated biphenyls, chlorophenols and anilines.

Aniline Compounds↗
PubMed2000

[Effects of different loading exercises on endogenous sex hormones and their metabolites].

Small loading and strengthening exercises were performed by 22 athletes(11 males and 11 females). The levels of serum hormones and their urinary metabolites were detected. The results showed that the serum levels of testosterone(T), free testosterone(FT) and 17-OH-progesterone(17-OHP) in male athletes exhibited no conspicuous changes. The excretion of their urinary androsteroids was significantly increased after exercises(P < 0.05), but significantly decreased in the recovery period(P < 0.05). After small loading exercises, their serum levels of T and FT revealed no obvious changes except those of 17-OHP which evidently increased in female athletes(P < 0.05); whereas the excretion of their urinary steroid hormones declined promptly after the exercises and in the recovery period (all P < 0.05). After strengthening exercises, the serum levels of T, FT and 17-OHP decreased distinctly in female athletes (all P < 0.05), the excretion of their urinary steroid hormones raised clearly after training and in the recovery period(P < 0.05). Therefore, it is suggested that the secretion and metabolism of sex hormones in the organism are influenced by the loading quality of sports during training, and sexual difference exists simultaneously.

17-alpha-Hydroxyprogesterone↗
PubMed2000

Spectroscopic studies of copper(II) and iron(II) complexes of adriamycin.

The complexes of adriamycin (ADM) with Cu(II) and Fe(II) have been studied by visible absorption, circular dichroism (CD) and fluorescence spectra, respectively. In Tris buffer at pH 7.0, either metal ions forms a single species with adriamycin: Cu(ADM)2 or Fe(ADM)3. Interaction of these two complexes with various biological molecules has been examined. It is shown that some amino acids, glutathione and albumin are able to remove the Cu(II) ion from Cu(II)-ADM complex, releasing the free drug. However, Fe(II)-ADM keeps in an undissociated form under the same conditions. The possibility of Fe(II) ADM as a new alternative drug has been discussed.

Antibiotics, Antineoplastic↗
PubMed2000

Ferrocenecarboxaldehyde labeled DNA probe for the study on DNA damage and protection.

A ferrocenecarboxaldehyde (FCA) labeled DNA probe is used for the first time in the study of DNA damage and protection. The electrochemically active reagent FCA was labeled successfully on to a denatured calf-thymus DNA by 1-ethyl-3- (3-dimethyl-aminopropyl) carbodiimide (EDC). The FCA labeled DNA probe was used to hybridize with the sample DNA sequence accumulated on the surface of a graphite electrode. The anodic peaks of the FCA bound to the double-stranded DNA (dsDNA) by differential pulse voltammetry (DPV) were used for the detection of DNA damage and protection. Thiourea, sodium benzoic acid and isopropanol can decrease DNA damage by hydroxyl radicals, and their protection efficiencies are discussed.

Animals↗
PubMed2000

Photokinetic voltammetric method for the determination of thiocyanate.

Thiocyanate traces have a strong inhibitory effect on the oxidation of Neutral Red by potassium bromate under UV irradiation in diluted phosphoric acid. Neutral Red exhibits a sensitive second derivative oscillopolarographic wave at -0.6 V(vs. SCE) in diluted phosphoric acid and sodium acetate solution. The oscillopolarographic behavior of Neutral Red was selected as indicator component for its photo-activated oxidation. The photochemical reaction rate equation was determined. A detection limit of 0.3 ng mL(-1) (3sigma/k) and a linear calibration curve from 2.0-48.0 ng mL(-1) thiocyanate were obtained. The method was applied to the determination of thiocyanate in urine, saliva and serum with satisfactory results.

Journal Article↗
PubMed2000

Catalytic determination of ultra trace amounts of tellurium by 2.5-order differential oscillopolarography.

A highly sensitive and selective catalytic method with 2.5-order differential oscillopolarographic detection is described for the determination of tellurium, based on its activation effect on the slow Pd(II)-catalyzed reaction of sodium hypophosphite and methyl red in hydrochloric acid medium at 100 degrees C. Methyl red exhibited a sensitive 2.5-order differential polarographic wave at -0.35 V (vs. SCE) in the supporting electrolyte of pH 5.0 acetate buffer solution and was chosen as the indicating component for the indirect determination of tellurium. A calibration curve of tellurium in the range of 0.02-2.0 ng mL(-1) was obtained by the fixed-time procedure. The detection limit was 0.007 ng mL(-1). Possible interferences by co-existing substances were examined. The new method has been used to analyze trace tellurium in organs of white mice with satisfactory results. RSD was 0.54% to 2.10%, recovery 98.4% to 95.7%. The mechanism is also discussed.

Azo Compounds↗
PubMed2000

Purification of ethoxyquin and its two oxidation products.

2,6-Dihydro-2,2,4-trimethyl-6-quinolone (QI) and 1,8'-bis(1, 2-dihydro-6-ethoxy-2,2,4-trimethylquinoline) (DM) are two oxidation products of 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline (ethoxyquin, EQ). This paper describes several methods for the purification of technical grade EQ and for the production of pure QI and DM as standards with the purity required (>99%) for calibration of quantitative determination methods. EQ of high purity was obtained through vacuum distillation followed by a quick column chromatographic purification on silica gel. Preparative scale purity DM could be obtained through recrystallization from methanol, but QI could be purified only by a high-pressure liquid chromatographic method.

Chromatography, High Pressure Liquid↗
PubMed2000

A cluster of mutations disrupt the avirulence but not the virulence function of AvrPto.

avrPto in Pseudomonas syringae pv. tomato encodes an avirulence protein that triggers race-specific resistance in tomato plants carrying Pto. The AvrPto protein is secreted from P. syringae pv. tomato to plant cells through the type III secretion pathway and activates race-specific resistance by a direct interaction with the Pto protein. Here we report that avrPto enhances the virulence of P. syringae pv. tomato in a strain-dependent manner in tomato plants lacking Pto. To determine whether the virulence function can be structurally separated from the avirulence function, we examined the virulence activity of a group of AvrPto mutants that carry single amino acid substitutions and lack the avirulence activity on tomato plants. Three mutants that were clustered in the center of AvrPto exhibited virulence activity in tomato plants with or without Pto. The rest of the mutations abolished the virulence. The identification of these mutants suggested that the avirulence function of AvrPto can be structurally separated from the virulence function.

Amino Acid Motifs↗
PubMed2000

Measurement of acoustic dispersion using both transmitted and reflected pulses

Traditional broadband transmission method for measuring acoustic dispersion requires the measurements of the sound speed in water, the thickness of the specimen, and the phase spectra of two transmitted ultrasound pulses. When the sound speed in the specimen is significantly different from that in water, the overall uncertainty of the dispersion measurement is generally dominated by the uncertainty of the thickness measurement. In this paper, a new water immersion method for measuring dispersion is proposed which eliminates the need for thickness measurement and the associated uncertainty. In addition to recording the two transmitted pulses, the new method requires recording two reflected pulses, one from the front surface and one from the back surface of the specimen. The phase velocity as well as the thickness of the specimen can be determined from the phase spectra of the four pulses. Theoretical analysis and experimental results from three specimens demonstrate the advantages of this new method.

Journal Article↗
PubMed2000

Dominant role of cAMP in regulation of microvessel permeability.

We reported previously that increasing cAMP levels in endothelial cells attenuated ATP-induced increases in hydraulic conductivity (L(p)), and that the activation of cGMP-dependent pathways was a necessary step to increase L(p) in response to inflammatory mediators. The aim of the present study was to evaluate the role of basal levels of cAMP in microvessel permeability under resting conditions and to evaluate the cross talk between cAMP- and cGMP-dependent signaling mechanisms in regulation of microvessel permeability under stimulated conditions, using individually perfused microvessels from frog and rat mesenteries. We found that reducing cAMP levels by inhibition of adenylate cyclase or inhibiting cAMP-dependent protein kinase through the use of H-89 increased basal L(p) in both frog and rat mesenteric venular microvessels. We also found that 8-bromocAMP (8-BrcAMP, 0.2 and 2 mM) was sufficient to attenuate or abolish the increases in L(p) due to exposure of frog mesenteric venular microvessels to 8-BrcGMP (2 mM) and ATP (10 microM). Similarly, in rat mesenteric venular microvessels, application of 8-BrcAMP (2 mM) abolished the increases in L(p) due to exposure to 8-BrcGMP alone (2 mM) or with the combination of bradykinin (1 nM). In addition, application of erythro-9-(2-hydroxy-3-nonyl)adenine, an inhibitor of cGMP-stimulated phosphodiesterase, significantly attenuated both 8-BrcGMP- and bradykinin-induced increases in L(p). These results demonstrate that basal levels of cAMP are critical to maintaining normal permeability under resting conditions, and that increased levels of cAMP are capable of overcoming the activation of cGMP-dependent pathways, therefore preventing increases in microvessel permeability. The balance between endothelial concentrations of these two opposing cyclic nucleotides controls microvessel permeability, and cAMP levels play a dominant role.

8-Bromo Cyclic Adenosine Monophosphate↗
PubMed2000

Leukocyte adhesion and microvessel permeability.

To investigate the direct effect of leukocyte adherence to microvessel walls on microvessel permeability, we developed a method to measure changes in hydraulic conductivity (L(p)) before and after leukocyte adhesion in individually perfused venular microvessels in frog mesentery. In 19 microvessels that were initially free of leukocyte sticking or rolling along the vessel wall, control L(p) was measured first with Ringer-albumin perfusate. Blood flow was then restored in each vessel with a reduced flow rate in the range of 30-116 microm/s to facilitate leukocyte adhesion. Each vessel was recannulated in 45 min. The mean number of leukocytes adhering to the vessel wall was 237 +/- 22 leukocytes/mm(2). At the same time, L(p) increased to 4.7 +/- 0.5 times the control value. Superfusion of isoproterenol (10 microM) after leukocyte adhesion brought the increased L(p) back to 1.1 +/- 0.2 times the control in 5-10 min (n = 9). Superfusing isoproterenol before leukocyte adhesion prevented the increase in L(p) (n = 6). However, the number of leukocytes adhering to the vessel wall was not significantly affected. These results demonstrated that leukocyte adhesion caused an increase in microvessel permeability that could be prevented or restored by increasing cAMP levels in endothelial cells using isoproterenol. Thus cAMP-dependent mechanisms that regulate inflammatory agent-induced increases in permeability also modulate leukocyte adhesion-induced increases in permeability but act independently of mechanisms that regulate leukocyte adhesion to the microvessel wall. Application of ketotifen, a mast cell stabilizer, and desferrioxamine mesylate, an iron-chelating reagent, attenuated the increase in L(p) induced by leukocyte adhesion, suggesting the involvement of oxidants and the activation of mast cells in leukocyte adhesion-induced permeability increase. Furthermore, with the use of an in vivo silver stain technique, the locations of the adherent leukocytes on the microvessel wall were identified quantitatively in intact microvessels.

Adrenergic beta-Agonists↗
PubMed2000

Suppression of D-galactosamine-induced liver injury by mushrooms in rats.

Six species of edible mushroom were found to suppress D-galactosamine-induced enhancement of plasma alanine and aspartate aminotransferase activities when powdered mushrooms were added to the diet (5%) and fed to rats for 2 wk. Grifola frondosa exhibited the most potent effect in a dose-dependent manner. A significant effect was observed only from the water-soluble low-molecular-weight fraction of G. frondosa. The results indicate that several mushrooms possess a protective effect against liver injury induced by D-galactosamine.

Agaricales↗