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Biomedical subjects

P Hartvig

Publications and source records attributed to P Hartvig.

At least 217 records · Page 12Linked to original sources
PubMed1978

A selected ion monitoring method for the determination of pethidine and norpethidine in plasma. Comparison with a gas chromatographic method using electron capture detection.

An analytical procedure for the simultaneous determination of pethidine and norpethidine in plasma by selected ion monitoring is described. The method has a capacity of 15--20 analyses per hour and can be used to determine pethidine and norpethidine down to 25 ng ml(-1) and 5 ng ml(-1), respectively. The selected ion monitoring method has been compared with a method based on electron capture detection after analysis of pethidine and norpethidine in spiked plasma samples and in plasma from patients. The two methods are capable of performing selective and accurate determinations of pethidine and norpethidine, in the concentration ranges obtained in man after a single therapeutic dose of pethidine.

Chromatography, Gas↗
PubMed1978

Untreated psychiatric disability. A study of disabled persons with major psychiatric health impairment, having never received psychiatric treatment.

The presented investigation, from a county seemingly representative of the average for Norway, showed that at least 15% of male Disability Pensioners with a psychiatric primary diagnosis, become pensioned without having had any psychiatric treatment. The purpose of the investigation was to describe this group of untreated psychiatric disabled, both numerically and regarding social, personal and nosological factors. It was found that the absence of treatment was due to lack of opportunity, but to the pensioner's own attitude, a stubborn resistance to being defind as a psychiatric patient. This is enhanced by a general tendency in our culture to choose, when possible, the role of a somatic patient rather than that of a psychiatric patient. The untreated group of male Disability Pensioners had a normal distribution of social status and stability, and showed a strong preponderance of neurosis-like conditions, combined with pains and other symptoms from the musculo-skeletal system in 51% of the clients. Among War Pensioners there were also found many untreated psychiatric disabled, especially ex-wartime seamen, showing a surprisingly constant syndrome of mixed anxiety and asthenia. The material comprised 150 males, 101 applicants for Disability Pension and 49 for War Pension, all untreated.

Adolescent↗
PubMed1978

Absorption of 8-hydroxyquinolines through the human skin.

The skin absorption of clioquinol and chloroquinaldol in ointment or cream base was studied in fourteen patients with widespread dermatoses. A serum concentration in the range of 0.3--1.3 microgram/ml using clioquinol and 0.05--0.1 microgram/ml with chloroquinaldol was reached by the second day and persisted throughout the topical treatment. The mean serum half-life of clioquinol and of chloroquinaldol was estimated to 25 and 6 hours, respectively. Local application of zinc oxide ointment after the end of the treatment chelate-bound the skin deposits of clioquinol and lowered the serum half-life to 11 hours. The mean daily urinary excretion of the applied amount of clioquinol and chloroquinaldol was 4.5 and 6%, respectively.

Administration, Topical↗
PubMed1977

Simultaneous determination of therapeutic plasma concentrations of pethidine and norpethidine in man by electron capture gas chromatography.

An analytical procedure for the simultaneous determination of pethidine and its N-Demethylated metabolite, norpethidine, in plasma is described. Pethidine and norpethidine are separated by partition chromatography, converted to the trichloroethyl carbamate with trichloroethyl chloroformate and determined by electron capture gas chromatography. The smallest amounts of pethidine and norpethidine determined by the method were 10 and 2 ng, respectively, in 0.1 ml plasma. The relative standard deviation in the determination of 50 ng of pethidine and 40 ng of norpethidine in 0.1 ml plasma wwere 5.8% (n = 8) and 6.3% (n = 10), respectively. The method was used to determine plasma levels of pethidine and norpethidine in three patients who received subcutaneous doses of pethidine 50-75 mg for postoperative pain. The peak levels of pethidine were found to be in the range 200-400 ng/ml, with a plasma half-life of the order of 4 hours. The levels of norpethidine were low.

Chromatography, Gas↗
PubMed1976

Determination of free fatty acids as pentafluorobenzyl esters by electron capture gas chromatography.

A method for the determination of free fatty acids in minute serum samples has been developed. The acids are esterified by extractive alkylation, using tetrabutylammonium as a counter ion and pentafluorobenzyl bromide as alkylating reagent. The derivatization of palmitic acid required a reaction time of 25 min. The excess of pentafluorobenzyl bromide is removed by coupling it with a phenoalkylamine and extraction of the product into an acidic aqueous phase. Quantitation is carried out by gas chromatography with electron capture detection. The precision achieved in the determination of 6.2 mug of palmitic acid in 50 mul of mouse serum was 6.1% (S.D.).

Alkylation↗
PubMed1976

Determination of pethidine in plasma by electron capture gas chromatography after reaction with trichloroethyl chloroformate.

Pethidine (meperidine) is determined in plasma by electron capture gas chromatography after derivatization with trichloroethyl chloroformate. The analytical procedure involves extraction of pethidine and the internal standard from plasma and their separation from metabolites by partition chromatography. After purification of the eluate, the derivatization is accomplished with trichloroethyl chloroformate in the presence of anhydrous sodium carbonate. The reaction mixture is further purified with methanolic alkali before gas chromatographic analysis. Optimum conditions for extraction and derivatization, as well as the sensitivity and selectivity of the method are discussed. Owing to the high sensitivity the pethidine levels are determined in 0.1 ml of plasma. The smallest amount of pethidine determined by the method was 100 ng/ml. The relative standard deviation at the 50-ng level of pethidine added to 0.1 ml of plasma was 5.8%(n =8).

Buffers↗
PubMed1976

Determination of plasma amitriptyline by electron-capture gas chromatography after oxidation to anthraquinone.

A selective procedure is described for the determination of amitriptyline in plasma. The method involves extraction, separation of amitriptyline from its metabolites and subsequent oxidation by ceric sulphate in 5.4 M sulphuric acid. The oxidation product, anthraquinone, is determined by means of electron-capture gas chromatography. The metabolites were separated by a column chromatographic extraction technique. The choice of oxidation reagent, optimum conditions for the oxidation, and the electron-capture properties of anthraquinone are discussed. The method can be used to determine down to 2 ng of amitriptyline in a plasma sample; the relative standard deviation at the 50-ng level was 4.0% (n = 8). The levels of amitriptyline found in a series of plasma samples are compared with those obtained by gas chromatography with use of nitrogen-specific detection; the two techniques gave coincident results.

Amitriptyline↗
PubMed1976

Electron-capture GC determination of subnanogram amounts of emepronium bromide in serum.

Barium peroxide in an acidic medium was utilized to increase the sensitivity in the benzophenone method for the determination of the quaternary ammonium compound emepronium bromide. The method is comprised of ion-pair extraction, oxidation, and quantitative determination of benzophenone by electron-capture GC. By employing small extraction and reaction volumes, the method was used in the 0.2--8-ng/ml range with a relative standard deviation of 2.5% at the 1-ng/ml level. The application of the method to human serum samples after a single oral dose demonstrated that the elimination phase for emepronium in serum had a half-life of 7--11 hr.

Administration, Oral↗