Search PubMed⌕ Search

Biomedical subjects

P H Anderson

Publications and source records attributed to P H Anderson.

13 recordsLinked to original sources
PubMed1979

The biological selenium status of livestock in Britain as indicated by sheep erythrocyte glutathione peroxidase activity.

The reliability of erythrocyte glutathione peroxidase activity as an indicator of selenium status in livestock is discussed. Based on this measurement, a survey is described of the biological selenium status of sheep on each of 329 farms in Britain. Results showed that 47 per cent of these farms were probably unable to provide grazing livestock with sufficient selenium to maintain blood levels greater than 0.075 microgram per ml. Increased selenium deficiency from the increasing use of home grown feeds as a major constituent of livestock rations may be causally related to the increase of white muscle disease and other selenium responsive diseases in Britain.

Animals↗
PubMed1977

Biochemical indicators of liver injury in calves with experimental fascioliasis.

Twenty calves were infected with 1000 metacercariae of Fasciola hepatica, the activities of 10 enzymes in plasma or serum were assayed and concentrations in serum of proteins, urea and bilirubin were determined. These values were compared with control data obtained from 14 uninfected calves. Aspartate aminotransferase, lactate dehydrogenase, sorbitol dehydrogenase, glutamate dehydrogenase, ornithine carbamoyl transferase and gamma-glutamyl transpeptidase activities increased in infected calves. Total serum protein increased, albumin decreased, globulin increased and the albumin/globulin ratio was decreased in infected calves. Plasma alanine aminotransferase, leucine aminopeptidase, alkaline phosphatase and cholinesterase activities and serum concentration of urea and bilirubin were unaffected. It was concluded that glutamate dehydrogenase and gamma-glutamyl transpeptidase were the most sensitive indicators of liver cell damage in fascioliasis.

Animals↗
PubMed1976

Some observations on "paralytic myoglobinuria" of cattle in Britain.

Characteristics of 44 outbreaks of "paralytic myoglobinuria" in yearling cattle reported to veterinary investigation centres during 1974 and 1975 are described. The clinical syndrome and associated nutrition and management of cattle are discussed in the light of present knowledge of this disease with particular reference to the diagnostic and epidemiological value of plasma creatine phosphokinase and blood glutathione peroxidase activities.

Animals↗
PubMed1975

Radioimmunoassay of plasma testosterone, with use of polyethylene glycol to separate antibody-bound and free hormone.

We have developed a reliable radioimmunoassay for testosterone in plasma, polyethylene glycol ("Carbowax 6000") being used to separate antibody-bound and free hormone. Testosteron is separated from interfering steroids, notably dihydrotestosterone, by liquid-liquid partition chromatography on infusorial earth (Celite). The assay is sensitive (9 pg for standards), precise, and accurate. The lowest measurable concentration fo testosterone is 350 ng/liter for plasma from men and 70 ng/liter for plasma from women. Intra- and inter-assay coefficients of variation were 6.9% and 9.7%, respectively, for plasma from men, and 9.6% and 11.8%, respectively, for plasma from women. Our method for separating antibody-bound and free hormone is practical and convenient and may be generally applicable to all radioimmunoassays of steroid hormones in plasma.

Antibodies↗