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Biomedical subjects

P Gulick

Publications and source records attributed to P Gulick.

9 recordsLinked to original sources
PubMed2000

Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin roots.

An abundant 17 kDa protein which was isolated and characterized from 10-day old healthy root tissue of white lupin (Lupinus albus) proved to have a high sequence similarity to pathogenesis-related proteins found in other species. Subsequently, a corresponding clone (LaPR-10) was identified in a cDNA library prepared from the same tissue that exhibited a high amino acid sequence similarity to a number of the PR-10 family proteins. The clone contains an open reading frame encoding a polypeptide of 158 amino acids, with a predicted molecular mass of 16,905 Da and an isoelectric point of 4.66. Southern blot analysis indicates that LaPR-10 is likely a single-copy gene, or a member of a small gene family. The clone was expressed in Escherichia coli, and its protein product was purified to near homogeneity. Both the native and the recombinant proteins were immunorecognized by antibodies raised against pea PR-10 proteins, and exhibited a ribonucleolytic activity against several RNA preparations, including lupin root total RNA. Characterization of its enzymatic properties indicates that the LaPR-10 protein belongs to the class II ribonucleases. We present evidence that the white lupin 17 kDa protein is constitutively expressed during all stages of root development and, to a lesser extent, in other plant parts. In addition, we demonstrate the presence, in the LaPR-10 amino acid sequence, of a number of motifs that are common to most PR-10 proteins, as well as a RGD motif that is shared only with the alfalfa SRG1 sequence.

Amino Acid Sequence↗
PubMed1998

Dermatomyositis associated with fallopian tube carcinoma.

Dermatomyositis (DM) is an idiopathic inflammatory myopathy associated with characteristic cutaneous and extracutaneous manifestations, including malignancy. Primary fallopian tube carcinoma (FTC) is the least common site of origin for a malignant neoplasm of the female genital tract. This report describes the first documented case of DM and concurrent FTC in the United States. A 62-year-old woman presented with DM and was subsequently found to have FTC. During her clinical course, she had improvement in strength and function with treatment of the underlying FTC, which also correlated with lower creatinine phosphokinase levels. An association between DM and FTC may exist because of significant functional strength improvements after tumor removal and chemotherapy.

Activities of Daily Living↗
PubMed1994

Cloning and regulation of flavonol 3-sulfotransferase in cell-suspension cultures of Flaveria bidentis.

Flaveria spp. accumulate flavonol sulfate esters whose biosynthesis is catalyzed by a number of position-specific flavonol sulfotransferases. Although the accumulation of sulfated flavonols appears to be tissue specific and developmentally regulated and to vary among related species, little is known about the mechanism of regulation controlling the synthesis of these metabolites. In the present work, we report the isolation of a cDNA clone from Flaveria bidentis (pBFST3) encoding flavonol 3-sulfotransferase (F3-ST), which catalyzes the first step in the biosynthesis of flavonol polysulfates. This clone (pBFST3) was expressed in Escherichia coli and produced an F3-ST with high affinity for the flavonol aglycones, quercetin, and its 7-methyl derivative, rhamnetin. In addition, the synthetic auxin 2,4-dichlorophenoxyacetic acid was shown to induce F3-ST enzyme activity and F3-ST mRNA transcript levels in cell cultures of F. bidentis. The F3-ST mRNA levels increased within the first 3 h, reaching a maximum after 24 h of treatment, and remained elevated for up to 48 h. Treatments with either quercetin 3-sulfate or quercetin 3,7,4'-trisulfate reduced F3-ST enzyme activity in cell cultures but had no effect on the transcript levels. These results are discussed in relation to the putative role of flavonoid conjugates in the regulation of auxin transport.

Amino Acid Sequence↗
PubMed1993

Mycobacterium marinum skin infections: two case reports.

Most infections of soft tissue injury sites are caused by Staphylococcus or Streptococcus and respond to beta-lactam antibiotics. Occasionally, a patient does not respond to routine antibiotics, and other possibilities must be considered. A detailed history of the event that caused the soft tissue injury can be important in the diagnosis. Two cases of Mycobacterium marinum soft tissue infection (ie, fish tank granuloma) are presented.

Female↗
PubMed1987

Gene induction and repression by salt treatment in roots of the salinity-sensitive Chinese Spring wheat and the salinity-tolerant Chinese Spring x Elytrigia elongata amphiploid.

An artificial amphiploid from a cross between salinity-sensitive bread wheat cultivar Chinese Spring and highly tolerant Elytrigia elongata (Host) Nevski (= Agropyron elongatum Host) shows enhanced salinity tolerance relative to Chinese Spring. Poly(A)(+) RNA was isolated from roots, expanding leaves, and old leaves from amphiploid and Chinese Spring plants prior to and after acclimation to high levels of NaCl in solution cultures. Two-dimensional gel electrophoresis of the in vitro translation products was used to compare these mRNA populations. The amphiploid had 10 mRNA species induced or enhanced and 8 species repressed in root tissue during acclimation to saline growth conditions. These 18 transcripts affected by salt treatment were also detected in wheat roots, but only 4 of these were similarly regulated. In Chinese Spring the acclimation to saline stress resulted in a marked change in the level of expression of 34 transcripts in root tissue; of these, 26 were detected in the amphiploid and only 6 were regulated as in the amphiploid. No differences were seen in gene expression between salt-treated and control plants in leaves and meristematic crowns and unexpanded leaves of the amphiploid.

Journal Article↗
PubMed1985

Analysis of heterogeneity in daunorubicin uptake by human leukemia cells using laser flow cytometry.

Heterogeneity in daunorubicin uptake by leukemic blast cells obtained from the bone marrow of 16 patients with acute leukemia (10 acute nonlymphocytic leukemia and 6 acute lymphocytic leukemia) was determined by laser flow cytometry following drug treatment in vitro for 1 hr. Fluorescence profiles of cellular anthracycline levels in the various samples indicated a marked heterogeneity in daunorubicin uptake and the range of detectable drug fluorescent cells was 34%-99%. A retrospective analysis of disease outcome in these patients who were treated with a daunorubicin or doxorubicin containing induction regimen indicated the following: (a) 8 of 11 patients who entered complete remission had greater than 80% of leukemic blast cells accumulating detectable amounts of daunorubicin; and (b) 3 of 4 patients who did not respond to chemotherapy had less than 40% of the leukemic blast cells accumulating detectable amounts of daunorubicin. These results suggest that laser flow cytometry may be useful in determining qualitative and quantitative differences in daunorubicin levels in heterogeneous tumor cell populations.

Adolescent↗
PubMed1980

Familial late complement component (C6, C7) deficiency with chronic meningococcemia.

Two patients with chronic meningococcemia were found to lack hemolytic complement, one because of C6 deficiency, the other because of C7 deficiency. In both cases family studies were consistent with inheritance of the deficiencies as non-HLA-linked, autosomal co-dominant traits. Functional studies showed the deficient sera to support monocyte chemotaxis but not phagocytosis or lysis of meningococci. Both patients have remained well following antibiotic treatment.

Adolescent↗