Biomedical subjects
P Gruppo
Publications and source records attributed to P Gruppo.
Platelet aggregation following electrical stimulation.
It was demonstrated that the previous in vitro electrical stimulation of human and rat platelet-rich plasma does not modify the subsequent response of platelets to the aggregating activity of ADP, thrombin, thrombofax or adrenaline. This is interesting in view of the fact that the electrical stimulation can induce clot retraction.
Electrical stimulation induces clot retraction after previous in vitro platelet aggregation.
The spontaneous clot retraction of platelet-rich plasma is inhibited by previous in vitro ADP-induced platelet aggregation. The electrical stimulation of the clot always restores a maximal clot retraction, even after a prolonged previous in vitro platelet aggregation.
Reptilase clot retraction induced by electrical stimulation.
Retraction of platelet rich plasma clotted by reptilase is induced by electrical stimulation. Optimal retraction is obtained by stimuli, applied for more than 4 min, with the following characteristics: intensity = 150 volts, duration = 50 msec each, frequency = 10/sec. Electrically induced reptilase clot retraction is shown to be inhibited by EDTA, EGTA, methyl-xanthines, PGE1, acetylsalicylic acid, indomethacin, but not by apyrase or by phosphoenolpyruvate-pyruvate kinase and MgCl2. The results indicate that electrical stimulation induces retraction of PRP clotted by reptilase by triggering off an increased availability of Ca2+ in the intracellular space.