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Biomedical subjects

P Griffiths

Publications and source records attributed to P Griffiths.

At least 163 records · Page 9Linked to original sources
PubMed1982

Dry-bed training in the treatment of nocturnal enuresis in childhood: a research report.

After one subject withdrew from an original group of twelve, eleven children with severe nocturnal enuresis were successfully treated by the Dry-Bed method. The children were aged five to nine years. The group median for the duration of treatment was four weeks, dryness being defined as two successive weeks without a bed-wetting incident. Training periods ranged from two to twenty weeks. Subsequently, two subjects relapsed, although organic and psychiatric factors respectively were probably responsible. At nine-month follow-up 73% of subjects were completely dry. The procedure made heavy demands on parents and clinician; especially in its initial phase. It was suggested that further feasibility studies are required of te Dry-Bed method of treatment in everyday service contexts.

Behavior Therapy↗
PubMed1982

Genetic control of the antibody response to poly(L Tyr, L Glu)-poly(DL Ala)--poly(L Lys) in mice: analysis of (low responder x low responder)F1 hybrids.

The antibody response to the synthetic polypeptide poly (L Tyr, L Glu)-poly (DL Ala)--poly (L Lys) designated (T,G)-A--L, was investigated in inbred, congenic, F1 and F2 hybrid strains of mice. The antibody response was analysed at both low (10 microgram) and high (50 microgram) immunizing doses of (T,G)-A--L. Antibodies were measured using both a modified Farr assay and a plate binding assay. At low immunizing doses it was found that all of the congenic and non-congenic (low responder x low responder) F1 hybrids were low responders. However, the quantitative antibody response of one non-congenic (low responder x low responder) F2 hybrid segregated in a 1:1 ratio of high responders to low responders, suggesting some form of complementation of (T,G)-A--L Ir genes. At high immunizing doses it was found that congenic and non-congenic (low responder x low responder) F1 hybrids were all high responders, indicating a complementation of Ir genes to (T,G)-A--L. This complementation was confirmed using two different routes of immunization, namely footpad and intraperitoneal. Furthermore the quantitative antibody responses of (low responder x low responder) F2 hybrids segregate in a 1:1 ratio of high responders to low responders. The class of antibodies produced to (T,G)-A--L in (low responder x low responder) F1 hybrids was determined by gel filtration on Sephadex G-200, and found to be predominantly IgG, with lesser amounts of IgM.

Animals↗
PubMed1981

The biological fate of sulphur colloid.

The in-vivo behaviour of sulphur colloid has been investigated using colloids labelled with 35S as well as 99m Tc. The rates of clearance of 35S and 99m Tc from the blood, the rates of accumulation in liver and bone and the distribution of the two radioisotopes in various organs are all markedly different. The results demonstrate that although technetium is rapidly removed from the blood stream and primarily accumulated in the liver the colloid particles themselves are broken down in vivo with the release of sulphur.

Animals↗
PubMed1981

The physical and chemical characteristics of sulphur colloids.

The characteristics of sulphur colloids have been investigated using colloids labelled with 35S as well as with 99mTc. The results support a model in which technetium is incorporated in the body of the particle rather than on its surface. Elemental sulphur on the surface of the particle is susceptible to attack both by sulphide ions and by protein and other materials containing --SH groups. The ready conversion of sulphur to soluble polysulphides means that sulphur colloid particles undergo conspicuous changes in diameter, both while standing in a closed vial, and on injection into body fluids. This behaviour casts doubts on the value of routine particle-size measurement as a quality control procedure.

Colloids↗
PubMed1981

Some isometric embedding and rigidity results for Riemannian manifolds.

In this note, we announce some new results concerning rigidity and isometric embeddings of Riemannian manifolds. A special case of the main result states that a general M(n) [unk] R(n+r), r</=(n - 1)(n - 2)/2, is uniquely determined up to finitely many constants by its induced ds(2).

Journal Article↗
PubMed1977

An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase.

1. 6-phosphogluconate dehydrogenase from sheep liver has been purified 350-fold by affinity chromatography with a final specific activity of 18 micronmol of NADP+/reduced min per mg of protein and an overall yield of greater than 40%. 2. A systematic investigation of potential ligands has been carried out: these included 6-phosphogluconate and NADP+, pyridoxal phosphate and several immobilized nucleotides. The results indicate that NADP+ is the most suitable ligand for the purification of 6-phosphogluconate dehydrogenase. 3. The effects of pH and alternative eluents have been examined in relation to the parameters known to affect the desorption phase of affinity chromatography; careful manipulation of the elution conditions permitted the separation of glucose 6-phosphate dehydrogenase, glutathione reductase and 6-phosphogluconate dehydrogenase from sheep liver on NADP+-Sepharose 4B. 4. A large-scale purification scheme for 6-phosphogluconate dehydrogenase is presented that uses the competitive inhibitors inorganic pyrophosphate and citrate as specific eluents.

Animals↗
PubMedDate not supplied

Orbital dystopia: attempts to evaluate the results of surgery.

This study has shown that surgery for hypertelorism or vertical orbital dystopia gives very satisfying results overall to the patients and their families and leads to a modest but highly significant objective improvement in appearance after surgery, as perceived by panels of laymen or hospital staff not known to the patients. The complexities of the methodology of panel assessments of appearance are discussed. The results of this study confirm the need to go beyond our traditional methods of assessing surgical outcome simply within our specialty.

Adolescent↗