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Biomedical subjects

P Gregory

Publications and source records attributed to P Gregory.

88 records · Page 5Linked to original sources
PubMed1979

Helminthosporium maydis Race T Toxin Induces Leakage of NAD from T Cytoplasm Corn Mitochondria.

The mechanism by which Helminthosporium maydis race T toxin inhibits respiration dependent on NAD(+)-linked substrates in T cytoplasm corn mitochondria was investigated. The toxin did not cause leakage of the soluble matrix enzyme malate dehydrogenase from the mitochondria or inhibit malate dehydrogenase or isocitrate dehydrogenase directly. The toxin did increase the permeability of the inner membranes of T cytoplasm, but not N cytoplasm, mitochondria to NAD(+). Added NAD(+) partially or fully restored toxin-inhibited electron transport in T cytoplasm mitochondria. Thiamin pyrophosphate had a similar effect when malate was the substrate. It was concluded that the inhibition of respiration of NAD(+)-linked substrates by the toxin is due to depletion of the intramitochondrial pool of NAD(+) and other coenzymes.

Journal Article↗
PubMed1976

Recent life changes and outcome of prolonged competitive stress.

To test the predictive utility of subjects' recent life changes with regard to outcome of prolonged competitive stress, the authors studied racers (N = 25) in a long distance Alaskan sled race, the Iditarod. Rank order correlations between subjects' 1-year life change units (LCU) total and adjusted place of finish proved significant by both Kendall's and Spearman's Test. Thus, lowe LCU scores correlated with better performance and higher scores with poorer performance. Differences between subgroupings of the racers are considered. The work suggests that the usefulness of recent life changes assessment may be extended beyong physical illness, particularly in the study of response and adaptation to prolonged stress.

Age Factors↗
PubMed1975

Plastid development in primary leaves of Phaseolus vulgaris. Development of plastid adenosine triphosphatase activity during greening.

The etioplasts of dark-grown bean leaves showed ATPase (adenosine triphosphatase) activity which had a pH optimum of 8.5, was stimulated by dithiothreitol and unaffected by light-triggering. Bean chloroplasts showed a low activity of dark-induced ATPase with a pH optimum of 8.5 and a substantial amount of light-triggered activity with a pH optimum of 8.0. The light-triggered activity depended on dithiothreitol and Mg2+ and was promoted by phenazine methosulphate. Light-triggered ATPase activity was completely inhibited by 20mum-dicyclohexylcarbodi-imide. Etioplasts developed light-triggered ATPase activity in response to 30 min illumination of the etiolated leaves. During the 48 h of light-induced greening of dark-grown leaves there was a 70% increase of the chloroplast ATPase activity found after light-triggering and a 30% fall in the dark-induced activity, both expressed on a per leaf basis. As the larger part of these changes occurred during the first 30 min of illumination, it is concluded that most or all of the chloroplast ATPase was present in the etioplast, a conclusion identical with that of Lockshin et al. (1971) for maize. During 48 h of greening there was a tenfold increase in the amount of thylakoid membrane in the leaf together with an 83% fall in the ATPase activity per m2 of thylakoid membrane, measured after light-triggering.

Adenosine Triphosphatases↗
PubMed1973

The Relation between Photophosphorylation and Delayed Light Emission in Chloroplasts.

One millisecond delayed light emission has been studied in isolated coupled lettuce (Lactuca sativa var. romaine) chloroplasts. Delayed light emission was increased upon addition of ferricyanide or 2,3,5,6-tetramethyl-p-phenylene diamine. In the presence of ferricyanide, the magnitude of the signal was increased by the addition of ADP (in the absence of orthophosphate), ATP, DI0-9, or phlorizin. The signal was also increased by the addition of NaCl and by the addition of NH(4)Cl in the presence of a high NaCl concentration. The signal of delayed light emission was decreased by the addition of gramicidin, valinomycin, and by the addition of NH(4)Cl in the presence of a low NaCl concentration.Phosphorylation, whether started by addition of ADP or by addition of glucose plus hexokinase plus ATP, caused a significant decrease in delayed light emission. It was concluded that the magnitude of delayed light emission reflects the size of the proton motive force across the thylakoid membrane. Calibration of delayed light emission by creating KCl gradients indicated that the value for the electrochemical potential gradient for H(+) in the presence of ferricyanide was at least 155 millivolts decreasing to 134 millivolts after the onset of phosphorylation.

Journal Article↗
PubMedDate not supplied

Digestibility rates of major and trace elements in pancreatic duct-ligated pigs.

Ligation of pancreatic duct in pigs leads to a severe maldigestion of calcium and magnesium. Substitution of missing enzymes results in a 'normalization' of the digestibility rates of these elements. In comparison to controls the pre-caecal phosphorus digestibility decreased, but the total digestibility rates increased, after ligation of the pancreatic duct. Furthermore, the total amount of absorbed phosphorus was significantly higher in pancreatic duct ligated pigs. Further studies have to be carried out to investigate, whether phosphorus in exocrine pancreatic insufficiency is excreted with urine, as in this study the observed Ca:P-ratio in blood was in a physiological range. Without a forced renal excretion of phosphorus, consequences and risks (e.g. secondary hyperparathyroidism) of the regulation have to be considered. Regarding the elements sodium, potassium and chloride, an increased faecal excretion could be observed in pancreatic duct ligated pigs. As a substitution with enzyme products led to digestibility rates similar to those in controls, no losses of electrolytes have to be feared. Even though pancreatic juice seems to have influences on the digestibility of investigated trace elements (copper, zinc, iron and manganese), it did not lead to severe imbalances in the corresponding mineral metabolism.

Animals↗
PubMedDate not supplied

Experiments to optimize enzyme substitution therapy in pancreatic duct-ligated pigs.

Ligation of the pancreatic duct in pigs leads to severe maldigestion and malabsorption of crude nutrients. Supplementation with 24 capsules of Creon (Solvay Pharmaceuticals GmbH, Hannover, Germany) per meal led to an increased digestibility of crude nutrients. With regard to optimization of the treatment of EPI no essential improvements can be achieved by adding omeprazol or lecithin to the diet. In pancreatic duct-ligated pigs the isolated addition of omeprazol led to an increase of the pre-caecal digestibility of crude fat and organic matter. With additional enzyme substitution, the application of omeprazol did not result in an improved fat digestibility. Isolated addition of lecithin to the diet resulted in a reduced total digestibility of crude fat. Offering the diet twice a day and using a higher frequency of enzyme applications (four or six instead of only two applications) had no effects on the digestibilty of crude fat or organic matter. According to the observations in pancreatic duct-ligated pigs, the addition of missing enzymes to the diet led to the best treatment results in EPI. Administration of omeprazol or a higher feeding frequency as well as the application of enzymes in small proportion of the whole meal or dosages given consecutively over the day showed no advantages. Furthermore, the present study suggests that the addition of lecithin cannot be recommended in EPI, when given diets with butter as the predominant fat source as in human dietetics.

Animal Feed↗
PubMedDate not supplied

Psychosexual therapy.

Sexual problems are more likely to originate from psychological or relationship difficulties rather than 'mechanical failure'. Here Peter Gregory reveals the often closed world of psychosexual therapy.

Female↗
PubMedDate not supplied

Patient assessment and care planning: sexuality.

In this article the author examines the purpose of discussing relationships and sexuality at initial patient assessment. Issues such as confidentiality, referral to specialist services and, most importantly, the benefit to the patient are discussed. The article begins with a definition of sexuality and describes a problem-solving approach which, with training, would enable nurses to address a patient's sexual and relationship difficulties knowing there is appropriate help available.

Confidentiality↗