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Biomedical subjects

P Gerlinger

Publications and source records attributed to P Gerlinger.

At least 55 records · Page 3Linked to original sources
PubMed1976

Messenger RNA translation in the presence of homologous and heterologous tRNA.

The effect of tRNA distribution in the cell on the rate of specific protein synthesis has been investigated. A tRNA-dependent cell-free protein-synthesizing system derived from Krebs-II ascites cells has been worked out. In this system it is possible to synthesize specific proteins (egg-white proteins or globin) by the translation of oviduct or reticulocyte messenger RNA in the presence of tRNA from homologous or heterologous tissues. The rate of translation of a give messenger RNA is optimal in the presence of tRNA from the homologous tissue. The lower level of protein synthesis obtained with an excess of heterologous tRNA can be overcome by adding the homologous tRNA. Nevertheless homologous isoaccepting species partly purified by a reversed-phase chromatography or a benzoylated DEAE-cellulose column cannot fully restore the optimal synthesis, eliminating the hypothesis according to which a particular tRNA species is involved in this phenomenon. The correct distribution of tRNA is necessary for optimal translation of a messenger RNA.

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PubMedDate not supplied

Correcting an immune-response deficiency by creating E alpha gene transgenic mice.

We have introduced a cloned Ek alpha gene into embryos of mice incapable of expressing their endogenous E alpha genes. The transcription of Ek alpha was accurate and tissue-specific in the resulting transgenic lines and, in macrophages, Ek alpha transcription could be induced by gamma-interferon. The injected gene conferred on the genetically deficient host strain the ability to mount an immune response to a synthetic polymer.

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PubMedDate not supplied

Tumour prevention and rejection with recombinant vaccinia.

Tumour-specific antigens (TSA; ref. 1) have been exploited in the diagnosis and imaging of human cancer and anti-TSA antibodies have therapeutic potential. Vaccination with TSA or anti-idiotypic (TSA) antibodies has also been used to control tumour growth in model systems. An effective immune response nevertheless demands copresentation of antigen with host histocompatibility determinants. We therefore examined whether live vaccinia virus recombinants expressing TSA in cells of the vaccinated host might better elicit tumour immunity. Polyoma virus (PY) is tumorigenic in rodents; because killed PY-transformed cells can elicit tumour immunity, a PY-specific TSA has been postulated. Tumorigenesis involves expression of three early PY proteins, large-T (LT), middle-T (MT) and small-T (ST), but their role as TSAs is unclear. We therefore expressed the three T proteins in separate vaccinia recombinants. Rejection of PY tumours was observed in rats immunized with recombinants expressing either LT or MT. Further, tumour-bearing animals could be induced to reject their tumours by inoculation of recombinants.

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PubMedDate not supplied

Isolation and biochemical properties of toxic tryptic peptides of ricinotoxin from Ricinus communis seeds.

Tryptic hydrolysis conditions of ricinotoxin were studied in order to produce not only digestion of this glycoprotein but also still toxic tryptic peptides. No hydrolysis was obtained without prior denaturation. The best conditions of denaturation were obtained with 0.2 M guanidine hydrochloride and, to a lower extent, by heat treatment at 90 degrees C during 6 minutes. The hydrolysates were fractionated on Sephadex G100 column. In each case highly toxic peptide fractions were obtained which showed, like native ricinotoxin, a strong inhibitory action on the in vitro protein synthesis ina cell-free eukaryotic system but were without any action on a prokaryotic cell-free system.

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