A 17-year-old adolescent had a recurrent episode of somnolence and morbid hunger (Kleine-Levin syndrome) three years after a first attack, from which he had spontaneously recovered. He was treated with 50 mg daily of clomipramine for the somnolence accompanied by disturbance of attention and memory. Under this treatment he developed thymoleptic symptoms with polyphagia, logorrhea and hyperactivity. Placed on a trial dose of at first 600 mg, then 400 mg carbamazepine daily the abnormal findings disappeared within a few days, and there has been no recurrence after some months. It is postulated, based on the observations of this case, that the Kleine-Levin syndrome, presumably a functional hypothalamic disorder, is closely related to the endogenous psychoses.

Adolescent↗

PubMed1989

Low heat resistance of Bacillus sphaericus spores correlated with high protoplast water content.

The low heat resistance (D100 = 0.554 min, z = 13.4 degrees C) of dormant lysozyme-sensitized spores of Bacillus sphaericus 9602 was correlated with a low protoplast wet density (1.305 g/ml) equivalent to a high protoplast water content (61.0%, wet weight basis). These values for these unusual spores were consistent with those correlated previously in 28 spore types of seven other species.

Bacillus↗

PubMed1989

[CT-guided biopsy: methods, results and complications].

Percutaneous CT-guided biopsies were carried out in 350 patients; on 291 occasions, 14- or 18-gauge and, on 59 occasions, an 0.95 mm cutting biopsy cannula was employed. Using the 14- and 18-gauge needles, a histological diagnosis was possible in 191 cases, a sensitivity of 82.7%. The 0.95 mm cannulas proved successful in 39 of the 59 punctures, producing a markedly lower sensitivity of 66.2%. The most common cause of an incorrect biopsy with large needles was bad position of the needle, whereas the small needles provided inadequate material. Complications which, however, did not require treatment, occurred in 5 patients (1.4%).

Biopsy, Needle↗

PubMed1988

Heat shock affects permeability and resistance of Bacillus stearothermophilus spores.

Heat shock of dormant spores of Bacillus stearothermophilus ATCC 7953 at 100 or 80 degrees C for short times, the so-called activation or breaking of dormancy, was investigated by separating the resulting spores by buoyant density centrifugation into a band at 1.240 g/ml that was distinct from another band at 1.340 g/ml, the same density as the original spores. The proportion of spores at 1.240 g/ml became larger when the original dormant spores were heated for a longer period of time, but integument-stripped dormant spores were quickly and completely converted to spores with a band at 1.240 g/ml. The spores with bands at both 1.240 and 1.340 g/ml were germinable faster than the original dormant spores and thus were considered to be activated. The spores with a band at 1.240 g/ml, which were considered to be fully activated, were apparently permeabilized, with a resulting complete depletion of dipicolinic acid, partial depletion of minerals, susceptibility to lysozyme action, permeation of the gradient medium, changed structural appearance in electron micrographs of thin-sectioned spores, and partly decreased heat resistance (D100 = 453 min) compared with the original dormant spores (D100 = 760 min). However, the fully activated spores with a band at 1.240 g/ml, although devoid of dipicolinic acid, still were much more resistant than germinated spores or vegetative cells (D100 = 0.1 min). The spores with a band at 1.340 g/ml, which were considered to be partly activated, showed no evidence of permeabilization and were much more heat resistant (D100 = 1,960 min) than the original dormant spores.(ABSTRACT TRUNCATED AT 250 WORDS)

Centrifugation, Density Gradient↗

PubMed1988

B-scan ultrasound examination of the carotid arteries within a representative population (MONICA Project Augsburg).

As part of the MONICA project carried out in Augsburg, 1388 male and female subjects, aged 25 to 65 years, participated in a B-scan ultrasound examination of the carotid arteries. The segments of the carotid arteries examined on both sides of the neck were the A. carotis communis, the A. carotis interna, and A carotis externa. As a result plaques were found in 330 subjects (23.9%). The presence of plaques in subjects is strongly correlated to age (increasing trend in both sexes, P less than 0.001) and the process of establishing plaques starts in men earlier than in women (P less than 0.01). For both sexes a different correlation structures is found for the relationship of cardiovascular risk factors to presence of plaques as well as to number of plaques in the carotid arteries. In men, total cholesterol (P less than 0.05) and personal history of diabetes, myocardial infarction, and stroke (P less than 0.01) were positively correlated with the presence of plaques while HDL/total cholesterol ratio shows a negative correlation (P less than 0.01). In women, none of the analyzed risk factors is found to correlate significantly with the presence of plaques. Regarding the number of vessels with plaques, a consistent and significant relationship with both hypertension and total cholesterol is seen in males and with hypertension in females. Comparing the different factors analyzed, age is obviously much more related to the occurrence of plaques than all the other risk factors together. Age is followed by a decrease in HDL cholesterol and, at a much lower level, by hypertension and smoking.

Adult↗

PubMed1986

Significance and limitations for diagnostics and follow-up.

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Angiography↗

PubMed1986

Heat resistance of bacterial spores correlated with protoplast dehydration, mineralization, and thermal adaptation.

Twenty-eight types of lysozyme-sensitive spores among seven Bacillus species representative of thermophiles, mesophiles, and psychrophiles were obtained spanning a 3,000-fold range in moist-heat resistance. The resistance within species was altered by demineralization of the native spores to protonated spores and remineralization of the protonated spores to calcified spores and by thermal adaptation at maximum, optimum, and minimum sporulation temperatures. Protoplast wet densities, and thereby protoplast water contents, were obtained by buoyant density sedimentation in Nycodenz gradients (Nyegaard and Co., Oslo, Norway). Increases in mineralization and thermal adaptation caused reductions in protoplast water content between limits of ca. 57 and 28% (wet weight basis), and thereby correlated with increases in sporal heat resistance. Above and below these limits, however, increases in mineralization and thermal adaptation correlated with increases in sporal resistance independently of unchanged protoplast water contents. All three factors evidently contributed to and were necessary for heat resistance of the spores, but dehydration predominated.

Adaptation, Physiological↗

PubMed1986

[Importance of leg phlebography for the diagnosis of venous diseases. Indications, technic and potential value].

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Humans↗

PubMed1985

Protoplast dehydration correlated with heat resistance of bacterial spores.

Water content of the protoplast in situ within the fully hydrated dormant bacterial spore was quantified by use of a spore in which the complex of coat and outer (pericortex) membrane was genetically defective or chemically removed, as evidenced by susceptibility of the cortex to lysozyme and by permeability of the periprotoplast integument to glucose. Water content was determined by equilibrium permeability measurement with 3H-labeled water (confirmed by gravimetric measurement) for the entire spore, with 14C-labeled glucose for the integument outside the inner (pericytoplasm) membrane, and by the difference for the protoplast. The method was applied to lysozyme-sensitive spores of Bacillus stearothermophilus, B. subtilis, B. cereus, B. thuringiensis, and B. megaterium (four types). Comparable lysozyme-resistant spores, in which the outer membrane functioned as the primary permeability barrier to glucose, were employed as controls. Heat resistances were expressed as D100 values. Protoplast water content of the lysozyme-sensitive spore types correlated with heat resistance exponentially in two distinct clusters, with the four B. megaterium types in one alignment, and with the four other species types in another. Protoplast water contents of the B. megaterium spore types were sufficiently low (26 to 29%, based on wet protoplast weight) to account almost entirely for their lesser heat resistance. Corresponding values of the other species types were similar or higher (30 to 55%), indicating that these spores depended on factors additional to protoplast dehydration for their much greater heat resistance.

Cell Compartmentation↗

PubMed1985

Protoplast water content of bacterial spores determined by buoyant density sedimentation.

Protoplast wet densities (1.315 to 1.400 g/ml), determined by buoyant density sedimentation in Metrizamide gradients, were correlated inversely with the protoplast water contents (26.4 to 55.0 g of water/100 g of wet protoplast) of nine diverse types of pure lysozyme-sensitive dormant bacterial spores. The correlation equation provided a precise method for obtaining the protoplast water contents of other spore types with small impure samples and indicated that the average protoplast dry density was 1.460 g/ml.

Bacillus↗

PubMed1985

[Causes of hypertension following kidney transplantation with special reference to arterial stenoses].

Hypertension is regarded as a significant complication in renal transplant recipients. Its frequency was 48,1% after cadaver donor and 40,6% after living donor transplantation. The etiology is multifactorial. A correlation could be demonstrated between blood pressure and graft function or presence or absence of the diseased kidneys while no influence was found with regard to steroid dosage in patients with long term graft function. After 575 renal transplantations with 147 angiographies arterial stenoses were observed in nine patients (1,6%) and corrected surgically.

Adolescent↗

PubMed1984

Seminal plasma characteristics during frequent ejaculation.

Seven young men provided a semen sample every 8 h for 2 days. Citric acid, zinc, acid phosphatase , spermine, fructose and protein were measured in the samples. Despite a dramatic reduction in semen volume, the concentration of the majority of the parameters remained constant during the period of study. The protein profile of the ejaculates, as assessed by 2-dimensional electrophoresis, also did not change. Levels of citric acid, zinc and acid phosphatase were closely correlated, while spermine, which is also believed to be of prostatic origin, showed no correlation with these other parameters. The spermine concentration of the ejaculates increased significantly during the period of high ejaculation frequency and returned to normal levels after a 3 day abstinence period. In view of the fact that spermine accumulation in tissues appears to be associated with growth and protein synthesis this increase in seminal spermine may reflect a stimulation of prostatic cell activity.

Acid Phosphatase↗

PubMed1984

Resistance, germination, and permeability correlates of Bacillus megaterium spores successively divested of integument layers.

A variant strain that produced spores lacking exosporium was isolated from a culture of Bacillus megaterium QM-B1551. Two additional spore morphotypes were obtained from the parent and variant strains by chemical removal of the complex of coat and outer membrane. Among the four morphotype spores, heat resistance did not correlate with total water content, wet density, refractive index, or dipicolinate or cation content, but did correlate with the volume ratio of protoplast to protoplast plus cortex. The divestment of integument layers exterior to the cortex had little influence on heat resistance. Moreover, the divestment did not change the response of either the parent or the variant spores to various germination-initiating agents, except for making the spores susceptible to germination by lysozyme. The primary permeability barrier to glucose for the intact parent and variant spores was found to be the outer membrane, whereas the barrier for the divested spores was the inner membrane.

Bacillus megaterium↗

PubMed1984

Microbiological sciences: a world view.

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Microbiology↗