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Biomedical subjects

P Gallian

Publications and source records attributed to P Gallian.

25 records · Page 2Linked to original sources
PubMed1997

Analysis of hepatitis C virus isolates using molecular and serological typing methods.

This study comprised 100 persons with antibodies to hepatitis C virus (HCV), including 77 intravenous drug users (IVDUs). They were tested with serological HCV typing assays (Murex HCV serotyping 1-6 assay; Chiron RIBA HCV Serotyping SIA). Patients with a positive polymerase chain reaction (PCR) for HCV (n = 66) were tested with genotyping molecular assays (Inno-Lipa HCV II test; Sorin GEN-ETI-K HCV typing assay). Comparison of the results of these tests showed that (a) 92% of samples could be typed by one test at least; 44% could be typed by all four tests; 88% could be typed by one serological test at least and 66% by one molecular test at least; (b) 81% of the samples successfully tested with both serological tests gave comparable results; 95% of the samples successfully tested with both molecular tests gave comparable results; (c) when serological and molecular tests yielded different results, sequences in the 5'-non-coding (5' NC) or E1 regions always confirmed the results of the molecular tests; (d) in case of discrepancy between the results of the molecular tests the E1 region sequences confirmed the Sorin test results. It is concluded that the molecular tests compared gave similar results. The fact that the Murex serological test gave comparable results in more than 80% of cases indicates that it is an alternative to the molecular tests for routine diagnosis. However, comparison of the results of this test with those obtained in patients consulting a hepatology department showed that it gave the best results in a population of patients not exposed repeatedly to HCV.

Adult↗
PubMed1996

Absent inhibition of hepatitis B virus replication by azidothymidine in patients chronically coinfected by HIV-1 and HBV.

The effect of Azidothymidine (AZT) in vivo on the replication of the Hepatitis B Virus (HBV) was studied in a population of 25 patients chronically coinfected by HBV and Human Immunodeficiency Virus Type 1 (HIV-1), and receiving AZT at the usual dosage. The drug effect was evaluated by sequential measurement of the HBV DNA level. No significant activity at short and medium term was found on HBV replication in either homosexuals or IV drug users chronically coinfected by HIV-1 and HBV.

Adult↗
PubMed1995

Evaluation of a chemiluminescent molecular hybridization assay for the detection and quantitation of hepatitis B virus-DNA in serum.

The Digene Chemiluminescent Molecular Hybridization Assay for the detection and quantitation of hepatitis B virus DNA (HBV-DNA) in serum was tested by comparing it with the Abbott HBV-DNA assay, which uses a radiolabelled probe. For the values of clinical interest, a relationship was found between the results (in pg/ml) from the Digene (Y) and the Abbott (X) assays: Y = 7X + 5. The cut-off value to obtain positive results with a specificity of 100% (as determined by polymerase chain reaction), was found to be 15 pg/ml for the Digene test and 10 pg/ml for the Abbott test. It was concluded that the Digene Chemiluminescence Assay is a fast convenient test, which is suitable for routine use.

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