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Biomedical subjects

P G Shivananda

Publications and source records attributed to P G Shivananda.

At least 19 recordsLinked to original sources
PubMed1995

Viral diarrhoea in a rural coastal region of Karnataka India.

A total of 106 children below 5 years of age admitted to the Kasturba Medical College Hospital Manipal Karnataka (South India) were investigated over a period of 6 months to determine the aetiological role of viruses in acute diarrhoea. Viral aetiological agents isolated were Rotaviruses in 12 (11 per cent) cases, Adenoviruses in 3 (3 per cent) cases, coronavirus and astroviruses in two (2 per cent) cases each. Non-viral isolates were Cryptosporidium and Salmonella typhimurium in two cases each, and Entamoeba histolytica and Shigella flexneri in one case each.

Adenoviridae Infections↗
PubMed1995

Effect of trace metals on synthesis of exoproteins and exotoxins by Staphylococcus aureus.

Staphylococcus aureus did not grow in chemically defined medium completely free of trace metals. At low concentrations all the trace metals showed stimulating effect on growth and exoprotein production. Increasing concentrations of Ca2+ and Mg2+ were growth stimulatory whereas Co2+, Cu2+, Fe3+, Mn2+, Ni2+ and Zn2+ were inhibitory. The amount of exoprotein and haemolytic exotoxins produced in relation to final viable count increased with increasing concentrations of growth inhibitory trace metals whereas it decreased with increasing concentrations of Ca2+ and Mg2+. Trace metals did not show significant influence on amount of haemolytic exotoxins produced in relation to total exoproteins. The results indicate presence of a common regulatory mechanism for all types of exoproteins produced by S. aureus.

Bacterial Proteins↗
PubMed1994

Fungal colonization in gastric ulcers.

Until now studies on fungal colonization of gastric ulcers were retrospective involving small series of patients. This prospective study of 50 patients with gastric ulcers (25 benign and 25 malignant) revealed colonization by Candida in 17 (34%) cases. There was no significant difference in colonization between benign and malignant ulcers. Follow up revealed no difference in healing of ulcers with or without fungal colonization.

Adult↗
PubMed1994

Bacteriological study of bronchoalveolar lavage in immunocompromised hosts.

Sixty bronchoalveolar lavage samples collected from lung cancer patients attending the Pulmonary Tuberculosis and Chest Diseases Unit of the Kasturba Medical College Hospital, Manipal; were cultured for both the aerobic and anaerobic organisms. Fifty nine samples yielded bacteria in pure culture. Pseudomonas aeruginosa (34.8%) was the commonest aerobe, Peptostreptococcus was the commonest anaerobe (45.2%) isolated. Bacteroides fragilis was isolated in (23.8%) of cases. Gentamycin was found to be effective against aerobes, Metronidazole and Rifampicin against anaerobes.

Bacteria, Aerobic↗
PubMed1994

Correlation of phospholipase A & enterotoxin production by Salmonella typhimurium with reference to virulence parameters.

Seventy strains of S. typhimurium along with 10 strains each of S. typhi and S. paratyphi B, 3 strains of S. enteritidis and 2 strains of S. senftenberg were examined for phospholipase A (PLA) activity. S. typhimurium positive for PLA activity (17.14%) were subjected to study of other virulence parameters like enterotoxin production, haemolytic activity, surface hydrophobicity and antibiotic sensitivity. A high degree of correlation was observed between PLA activity and enterotoxin production, antibiotic sensitivity and cell surface hydrophobicity respectively.

Child, Preschool↗
PubMed1994

Effect of trace metals on production of exoprotein and beta-lactamase by Staphylococcus aureus.

A minimum concentration of 0.01 mM of Mg2+ was essential for any growth of S. aureus. Of the trace metals, Mg2+ and Ca2+ were highly stimulatory for growth. All trace metals except Ni2+ in small concentrations were stimulatory for growth and exoprotein production. Amounts of exoprotein and beta-lactamase produced relative to the number of viable cells were maximum during the period of suboptimal proliferation of bacteria. Trace metals did not significantly influence the amount of beta-lactamase produced relative to exoprotein.

Bacterial Proteins↗
PubMed1994

Invasive maxillary aspergillosis in an otherwise healthy individual.

Invasive aspergillosis is usually seen in immunocompromised individuals. We report the case of a 56-year-old immunocompetent housewife with Aspergillus niger invading the ethmoid and sphenoid sinuses and extending to the anterior cranial fossa. A. niger grew on three occasions (from the biopsy, mass and dura mater). In spite of medial maxillectomy and ethmoidectomy followed by craniofacial resection and antifungal drugs (amphotericin B) the patient died on tenth post-operative day.

Aspergillosis↗
PubMed1994

Effect of magnesium on the physiology of Staphylococcus aureus.

The role of magnesium (Mg2+) in the metabolism of Staphylococcus aureus was studied using a chemically defined medium. At least 0.01 mM Mg2+ was required for any growth of S. aureus. Increasing concentration of Mg2+ up to 1.6mM steadily increased the bacterial growth. Glucose utilisation and acid production were also influenced by Mg2+. Excessive cobalt (Co2+) and zinc (Zn2+) were inhibitory to S. aureus growth and Mg2+ could reverse the inhibitory effect.

Cobalt↗
PubMed1994

Rapid identification of Salmonella typhimurium in stools by using 4-methylumbelliferone derivatives.

A total of 150 stool samples were collected from the Paediatric ward and screened for the presence of Salmonella typhimurium. A comparison of standard biochemical tests and rapid fluorescence method was done. The fluorescence method was found to be rapid, less time consuming and easy to perform whereas identification of Salmonella typhimurium using standard biochemical tests taken 18 to 24 hours.

Child↗
PubMed1993

Bacteriology of neonatal septicaemia in a rural referral hospital in south India.

Out of 640 suspected cases of neonatal septicaemia studied, bacteraemia was detected in 255 (40 per cent) of the infants. Gram negative organisms were predominant (56 per cent) with Pseudomonas, Citrobacter, and Klebsiella as the commonest pathogens. Among the Gram positive organisms both Staphylococcus aureus and Staphylococcus epidermidis were equally prevalent. Staphylococci were mainly responsible for early onset infections, whereas Salmonella typhimurium and Pseudomonas were the main organisms in late onset infections. Group B streptococcal infection was not encountered in this part of the country.

Age of Onset↗
PubMed1993

Molds in onychomycosis.

BACKGROUND: Onychomycosis is a major cause of nail dystrophy. The causative organisms in onychomycosis are dermatophytes, Candida and molds. A variety of molds have been isolated from nails. METHODS: Nail scrapings and clippings were collected from 100 cases and inoculated on slants containing SDA with cycloheximide 0.5 mg/mL, chloramphenicol 0.05 mg/mL, and SDA with chloramphenicol 0.05 mg/mL. RESULTS: The culture positivity rate for molds was 22%. The predominant mold isolates were Aspergillus species (86.4%, Fusarium oxysporum (4.5%), Curvularia species (4.5%) and Penicillium species (4.5%). CONCLUSION: Primary invasion of nails by molds can cause onychomycosis.

Female↗
PubMed1993

Emergence of multidrug-resistant Salmonella typhi in rural southern India.

Multidrug-resistant Salmonella typhi has spread to many parts of India, causing severe therapeutic problems. Of the 305 clinically suspected cases of enteric fever seen at Kasturba Hospital in Manipal, Karnataka between January 1990 and June 1991, Salmonella bacteremia was detected in 134 patients; 102 of these were caused by S. typhi. Eighty (78.4%) of the isolates from S. typhi-infected patients were resistant to conventional antibiotics used in the treatment of typhoid fever (i.e., ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole). No in vitro resistance was observed to ciprofloxacin, norfloxacin, and gentamicin. Major complications were seen in four patients infected by multiresistant S. typhi; three responded well to ciprofloxacin. The fourth patient died of hemorrhage and acute renal failure, even after receiving ciprofloxacin. In addition, less severe complications such as hepatitis and jaundice were observed in 12 other patients. Seventy-six of the multiresistant S. typhi belonged to phage type O biotype II and four were of untypeable Vi strains. The emergence of multidrug-resistant S. typhi has necessitated the use of fluoroquinolones in the therapy for enteric fever.

4-Quinolones↗
PubMed1993

Acinetobacter sepsis in neonates.

Twenty-six neonates were diagnosed to have acinetobacter sepsis during 1986-90, representing 6.5% of all cases of bacteriologically proven sepsis. Of these 19 neonates were low birth weight (LBW) 12 were small for gestational age (SGA). Nineteen neonates had early-onset sepsis. The male to female ratio was 9:17. The hematological profile was suggestive of sepsis in 17 cases. All infants had clinical evidence of multi system infection. Eleven babies died; the cases-fatality rate was 42.3%. Only 15/25 culture isolates were sensitive to gentamicin and resistance to other antibiotics was even more frequent. Acinetobacter was cultured from other sites: eye swabs, skin pustules and umbilical catheter tips. Environmental nursery surveillance cultures done during the study period yielded Acinetobacter once from a crib, but no cases of sepsis occurred around that time. The epidemiological features of this organism illustrate the value of vigilance and precautionary measures.

Acinetobacter↗
PubMed1993

A modified chemically defined medium for Staphylococcus aureus.

A chemically defined medium has been described which supported good growth of S. aureus. Optimal production of different exoproteins, viz. coagulase, acid phosphatase, proteinase, lipase, beta-lactamase and alpha-toxin was recorded in the medium.

Bacterial Proteins↗