Effects of hypergravity on "whole-blood" cultures of human lymphocytes.
The purpose of this paper is to present a detailed description of the effects of hypergravity on the mitogenic response of human lymphocytes to concanavalin A. The effect on cultures of lymphocytes isolated from peripheral blood are compared with those on whole-blood cultures obtained by diluting fresh blood with culture medium 1:10. Whole-blood cultures of lymphocytes from crew members will be investigated inflight on the Spacelab missions D-1 in 1985 and SLS-1 in 1987. In hypergravity there is an increase in lymphocyte activation of up to 500%. A similar increase can be induced by pre-incubating the cultures in hypergravity prior to exposure to concanavalin A at 1 G. The effect is less evident in cultures of isolated lymphocytes. The influence of autologous plasma and erythrocytes has also been investigated. Plasma and hypergravity have a synergistic and positive effect on lymphocyte activation, i.e. cultures of separated lymphocytes show the highest activation when incubated at 10 G and supplemented with autologous plasma. Conversely, erythrocytes depress lymphocyte activation.