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Biomedical subjects

P Franchimont

Publications and source records attributed to P Franchimont.

At least 325 records · Page 18Linked to original sources
PubMed1983

[Immunoenzyme assay of human cartilaginous proteoglycans and their antibodies].

An ELISA has been developed to measure human cartilaginous proteoglycans (PG) and their auto-antibodies. The assay is described step by step: successively the nature of the microtiter wells, the concentration of the PG to be coated, the optimal dilution of the antiserum, the length of various incubations and their respective temperature, are described. Standard curve obtained with purified PG is linear in a logit-log representation. The sensitivity of the assay is 2 ng/tube. Finally, biological fluids-sérum and synovial fluid-show a good parallelism with PG.

Autoantibodies↗
PubMed1983

[Labeling of beta-endorphin and beta-lipotropin with iodine 125].

5 micrograms of human beta-endorphin were labelled with 2 mCi 125I by the chloramine T technique. After two gel filtrations on Sephadex G-15 and on Sephadex G-50 in phosphate buffer with EDTA, Trasylol and mercapto-ethanol, a pure tracer was obtained with a specific activity about 150 microCi/ug. Kept at + 4 degrees C, the tracer remained utilizable for 30 days without loss of immunoreactivity. The labelling with lactoperoxydase and the use of another gel filtration method (filtration on Aca 202) gave a 125I beta-END tracer with the same immunoreactivity. The binding of this tracer to the antibody of an anti-beta-END antiserum diluted at 1/8000 was 32% with a non specific binding of 2%. 5 micrograms of human beta-lipotropin were labelled with 0.5 mCi 125I by the lactoperoxydase method. After two gel filtrations on Sephadex G-25 and on Sephadex G-75 in phosphate buffer with EDTA, Trasylol and mercapto-ethanol, a pure tracer with a specific activity of 140 microCi/micrograms was obtained. It remained utilizable for 30 days when kept at + 4 degrees C. Gel filtration on Aca 202 did not give good purification, while gel filtration on Aca 54 was good but slower than on Sephadex G-75. The binding to antibody in absence of unlabelled beta-LPH was 32% for an anti-beta-LPH antiserum diluted at 1/4000. The non specific binding was 2.5%.

Endorphins↗
PubMed1983

[In vitro incorporation of radioactive sulfur into proteoglycans of cartilage. Effects of various nonsteroidal anti-inflammatory agents].

Radioactive sulphur (35SO4) is incorporated in the proteoglycans of chicken embryonic cartilage. In fact, the molecular weight of the macromolecules which bind the sulphur corresponds to monomeric proteoglycans and proteoglycan-hyaluronic acid complexes. This incorporation does not take place when the cartilage has been previously killed. The capture of 35S is stimulated by human serum, in a dose-dependent way. At doses less then, equal to and greater than the maximal plasma concentrations observed following a single oral dose (600 mg), acetyl salicylic acid and sodium salicylate inhibit the incorporation of radioactive sulphur in the cartilage. Under the same conditions, tiaprofenic acid and oxametacine have no effect on this metabolic parameter.

Animals↗
PubMed1982

Organ cultures of mammalian testes. II. Meiotic chromosomes of adult mice in vitro.

Meiotic chromosomes of adult mice testes cultured in vitro have been studied. All meiotic stages are present and normal until the 8th day; suitable figures for chromosome studies may be obtained with routine cytogenetic methods. During the 9th and 10th day in vitro, the number of cells in meiotic progress falls abruptly. By the 14th day, only a few pachytene stages are still observed. This breakdown of meiosis, linked with the disappearance of the germ cell line, is not due to chromosome anomalies that could have arisen in the new environment. Labeling with tritiated thymidine suggests that spermatogonia do not enter meiotic division in vitro. Chromosomes observed with this technique belong to cells already involved in meiotic processes at time of the initiation of the culture.

Animals↗
PubMed1982

Hypopituitarism and idiopathic delayed puberty: a longitudinal study in an attempt to diagnose gonadotropin deficiency before puberty.

Fifty-five hypopituitary patients (43 boys and 12 girls) treated with human GH were studied longitudinally before and during puberty, occurring either spontaneously or induced with testosterone enanthate (100 mg/month, im) in boys and ethinylestradiol (10 micrograms/day, orally) in girls. In addition, 53 boys with idiopathic delayed puberty (IDP) were studied. Gonadotropin integrated responses (IRs) during 90 min after the iv injection of 25 micrograms/m2 LRH, bone ages (BA), and plasma levels of dehydroepiandrosterone sulfate and testosterone were determined at least yearly. Prepubertal hypopituitary patients with gonadotropin deficiency were characterized by: 1) a lowered FSH IR to LRH in most boys and in all girls; 2) a low LH IR for BA; 3) adrenarche either absent or delayed BA; 4) height age close to BA; and 5) the presence of several pituitary deficiencies. In contrast, most prepubertal hypopituitary patients without gonadotropin deficiency showed: 1) a normal FSH IR to LRH; 2) a normal or intermediate (greater than or equal to 75 mIU/ml . 90 min) LH IR for BA; 3) a normal adrenarche for BA; 4) a height age below BA; and 5) isolated GH or GH plus TSH deficiencies. A significant linear correlation was found between LH IR and the logarithm of plasma testosterone. The slopes and levels were similar in controls and hypopituitary boy without gonadotropin deficiency. In IDP, the level was significantly higher. All data obtained in these patients show that the increase in plasma testosterone and the clinical onset of puberty are delayed for the observed pubertal pattern of LH responsiveness. It is concluded that the study of several clinical and biological features, especially the gonadotropin IR to LRH, are of predictive value for the diagnosis of normal or deficient gonadotropic function in prepubertal patients with IDP and hypopituitarism.

Adolescent↗
PubMed1982

[Hypothalamo-hypophyseal maturation of puberty. Experimental data].

Data from the literature on hypothalamo-pituitary function in relation with sexual maturation have emphasized the role of several factors possibly involved in the modulation of LHRH and gonadotropin release. Here, the periodicity of LHRH signals seems to be very important. The mechanisms of hypothalamo-pituitary maturation at puberty seem to include the disappearance of an inhibitory control exerted by gonads on one hand and supra hypothalamic nervous system on the other hand. In relation with sexual maturation of male rats, we have studied the effects of estradiol upon the response of pituitary cells to LHRH in vitro, the effects of castration on hypothalamic LHRH and finally LHRH release from hypothalamus in vitro. Our observations suggest that the inhibitory controls are mainly effective through an hypothalamic action.

Animals↗
PubMed1982

[Tumor markers].

Several substances, referred to as tumor markers, are associated with neoplasms development. The specificity of these cancer related substances or antigens depends on their nature (onco-fetal antigens, placental antigens) or on their concentration and physico-chemical forms (hormones, exocrine products, enzymes,...). On the basis of physico-chemical, immunochemical and biochemical analogies which exist between these tumor markers and substances normally found at particular times of life, a classification of these markers may be proposed. Tumor markers are almost constantly found within carcinoma cells by immunocytochemical techniques and are secreted by carcinoma explants in culture medium. On the hand, the release of tumor markers in biological fluids (blood, cerebrospinal fluid, urines,...) is less frequently detected by sensitive methods such as radioimmunoassay. Several factors are responsible for this discrepancy between the intra-tumoral presence of tumor markers and the lower incidence of their detection in biological fluids. These factors are discussed. These tumor markers have attracted considerable attention from pathologists and clinicians. Thus, detection of these substances, especially by immunocytochemical methods, may be related to a situation of neoplastic transformation and allow a functional classification superimposed to histological classification superimposed to histological classification of tumors. Moreover, ectopic production of hormones and/or neuromediators explains some clinical symptoms in cancer processes. Furthermore, products of normal cell activity at the origin of cancer (hormones, enzymes, exocrine products) when evidenced within the neoplastic cells or within serum might constitute a hormonal dependence index useful for therapeutical orientation. Finally, tumor marker levels are related to the local and systemic extension of the neoplasia and may be considered as valid index of prognosis. The determination of the levels of these tumor markers provides a quantitative criterion of the evolution of the neoplastic disorder and for following the efficacy or inefficacy of treatment.

Adrenocorticotropic Hormone↗
PubMed1982

[Formation of human immunoreactive LMW and HMW-kininogens during coagulation. Comparison with bradykinin activity].

Variations of the levels of human HMW and LMW kininogens have been studied in serum and in plasma incubated in vitro during 30 hours, at three different temperatures: 4 degrees, 22 degrees and 37 degrees C. There is a small difference between the level of LMW kininogen in plasma and in serum, but the serum and plasma level of LMW kininogen are almost stable during the time of incubation at the three temperatures. However, HMW kininogen is reduced in serum to about fifty percent of its plasma level. It decreases in plasma and in serum during incubation, overall at 4 degrees C. A strict parallelism stays between the radioimmunoassay and the bioassay.

Biological Assay↗
PubMed1982

A double-blind crossover study of benoxaprofen and indomethacin in outpatients suffering from osteoarthrosis of knees.

Benoxaprofen (600 mg once a day) and indomethacin (25 mg t.i.d.) were compared in a double-blind, randomized, crossover study in 35 ambulatory hospital outpatients suffering from osteoarthritis of the knee. Six patients on each treatment withdrew because of gastric side effects (3 patients withdrew from both treatments) and a total of 5 patients withdrew because of lack of efficacy (2 on benoxaprofen and 3 on indomethacin). Twenty-two patients were suitable for analysis of efficacy and safety. The two drugs were shown to be comparable in efficacy by both the subjective and objective parameters used to assess pain, stiffness, and movement. However, because there was no washout period between administration of the two drugs, there was a tendency for the drug given second (8 benoxaprofen versus 14 indomethacin) to have greater efficacy. Side effects reported were mostly mild in nature and mostly gastrointestinal in origin. A significant reduction in levels of alkaline phosphatase (p less than 0.005) was noted during benoxaprofen therapy.

Aged↗