Search PubMed⌕ Search

Biomedical subjects

P Fondu

Publications and source records attributed to P Fondu.

At least 91 records · Page 5Linked to original sources
PubMed1984

Enzymes of purine metabolism in B-chronic lymphocytic leukemia.

In order to study if enzymes of purine metabolism could be used as cell markers in B-chronic lymphocytic leukemia (B-CLL), the activities of adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), and 5'-nucleotidase (5'N) were repeatedly measured in blood mononuclear cells from B-CLL patients and were compared to those obtained in normal controls. Enzyme activities in patients were also compared to other biological parameters indicative of B-CLL to activities of ADA and PNP in erythrocytes. Results show that B-leukemic cells display abnormal enzyme patterns: subnormal ADA activity is characteristic; 5'N activity is depressed in 60% of the cases but increased in 15%. An inverse relationship between PNP activity and corresponding lymphocytosis is observed in leukemic but not in normal cells. The enzymatic anomalies seem to be linked to the presence of an unusual peripheral lymphocytic population, induced by the leukemic process. Indeed, ADA and PNP are not abnormal in erythrocytes. In untreated nonevolutive patients, the enzyme profile tends to remain stable throughout the course of the illness; normalization of enzyme patterns in treated patients occurs only when therapy induces improvement in T and B cell distribution.

5'-Nucleotidase↗
PubMed1984

Thymidine kinase and thymidine phosphorylase activities in various types of leukaemia and lymphoma.

Thymidine kinase (TK) isoenzymes and thymidine phosphorylase (TP) activities have been measured in peripheral mononuclear cells of patients with acute lymphoblastic and monoblastic leukaemia or B-chronic lymphocytic leukaemia, as well as in normal subjects, and also in lymph node cells from patients with non-Hodgkin's lymphoma, with Hodgkin's disease and with benign adenopathies. TK1 isoenzyme activity was highest in acute lymphoblastic leukaemia and in centroblastic lymphoma. Then in progressively decreasing order appeared the Hodgkin's disease values, the centroblastic centrocytic lymphoma values and the benign reactive lymph node cell values. When compared to normal blood mononuclear cells, TP was greatly decreased in acute lymphoblastic leukaemia and slightly but significantly decreased in chronic leukaemia. Monoblastic cells exhibited a unique enzyme pattern; moderately increased TK1 activity and high TP activity. Our results suggest that both enzymes are indicative of the maturation status of leukaemic cells from B lineage. They demonstrate that in lymph node cells, TK1 reflects the proliferative status of both malignant and non-malignant cells and that in monoblastic cells the synthesis of dTMP through de novo synthesis is favoured.

Humans↗
PubMed1983

Burkitt's lymphoma--a correlated light and electron microscopic study on the malignancy in the CSF.

In a sporadic case of primary maxillar Burkitt's lymphoma and secondary spinal epidural dissemination, the massive malignancy in the CSF was investigated by light and electron microscopy. At both levels the CSF tumor cells were strikingly similar, with the undifferentiated lymphoblasts that characterize Burkitt's lymphoma in tissue. However, the starry-sky appearance in the CSF was produced more often by degenerating malignant cells than by histiocytes as classically described in tissue with Burkitt's lymphoma.

Burkitt Lymphoma↗
PubMed1982

Erythrocyte glycolysis in protein-energy malnutrition.

Erythrocyte glycolysis has been studied in the anaemia associated with protein-energy malnutrition (PEM) in Kivu. Several results were compatible with a lowering of the mean age of the erythrocyte population, notably raised levels of glucose-6-phosphate, hexokinase, Na+-K+- adenosinetriphosphatases and potassium, and low sodium concentration. Non-significant differences were observed for glucose utilization, lactate formation, and for concentrations of fructose-6-phosphate, fructose-1,6-diphosphate, adenosine diphosphate and pyruvate kinase; there was no gross disturbance of cation transport. The level of adenosine triphosphate was slightly decreased and that of 2,3-bisphosphoglycerate was not elevated, in spite of anaemia. The latter could not be explained by an instability of this metabolite. It is concluded that slight erythrocyte glycolytic abnormalities may occur in the anaemia of Kivu PEM, but that they are not the main cause of the haemolysis observed in this syndrome.

Adolescent↗
PubMed1982

Fanconi's anaemia. Simultaneous onset in 2 siblings and unusual cytological findings.

Fanconi's anaemia is reported in 2 siblings. The simultaneous onset of pancytopenia after possible exposure to common external agents suggest that both a frail genotype and environmental factors may be etiologically involved in the disorder. One of the children died after having developed a monoblastic leukaemia. This patient disclosed very striking cytological abnormalities, including a dyserythropoietic pattern and ropalocytosis. The physiopathology of the latter abnormality, which has hitherto not been noticed in Fanconi's anaemia, is uncertain.

Adolescent↗
PubMed1981

Oxidative haemolysis in protein malnutrition.

A study of the haemolytic anaemia observed in protein-energy malnutrition (PEM) in Kivu disclosed the following results. The in vitro resistance to oxidative aggressions of PEM patients' erythrocytes was decreased: when incubated with acetylphenylhydrazine, a higher percentage of the cells showed Heinz bodies, as compared with erythrocytes of local controls. Normal or increased activities were found for certain erythrocyte enzymes involved in the detoxification of activated oxygen: glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and glutathione reductase. The level of reduced glutathione was not decreased. Reduced activities were observed for two enzymes containing trace elements: glutathione peroxidase and superoxide dismutase. It is suggested that the shortened erythrocyte lifespan observed in PEM patients corresponds to an oxidative process which results from the decrease of both enzyme activities. The hypothesis that depletion of trace elements could be responsible for the decreased activity of those enzymes is discussed.

Adolescent↗
PubMed1981

Immunoblastic sarcoma of the B-cell type in the CSF. Light- and electronmicroscopic study of the malignant cells and unusual macrophages.

In a patient with systemic and nervous system IBS, the CSF cells were investigated by light and electron microscopy. An IBS of the B-cell type was identified in the CSF which proved, at both morphological levels, to be identical to that recorded in IBS in lymphoid tissue with regard to the malignant cell types and the absence of surface specialization. In contrast, unusual macrophages were observed in the CSF instead of the reticular elements associated with IBS in lymph nodes which present desmosomes. They demonstrated both phagocytosis and emperipolesis and also displayed autodesmosomes. The CSF phagocytes are discussed in relationship with the phagocytes described in the brain with malignant lymphoma.

B-Lymphocytes↗