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Biomedical subjects

P F Suraĭ

Publications and source records attributed to P F Suraĭ.

4 recordsLinked to original sources
PubMed1991

[The determination of alpha-tocopherol in blood serum].

A modified method for measuring vitamin E is described, making use of thin-layer chromatography with Silufol plates. Effects of various storage conditions of the blood serum on its vitamin E levels were examined. Vitamin E proved to be sufficiently stable at storage at -10 degrees C and defrosting under 20 degrees C. Comparison of this mode of storage with other ones has demonstrated its advantages. The method was tried in clinical practice in examinations of patients with pulmonary tuberculosis, chronic alcoholism, females suffering from infertility due to inflammations and healthy ones (controls) and found to be accurate, reproducible, and informative.

Alcoholism↗
PubMedDate not supplied

[Practical aspects of using silufol sheets to analyze fat-soluble vitamins].

The authors studied the mobility of the fat-soluble vitamins, A, D, K1 and beta-carotene in organic solvents and their mixtures on silufol plates, the influence of the chromatography conditions and the chamber saturation with the solvent vapors on the Rf values of fat-soluble vitamins, as well as the rate of beta-carotene tocopherol (vitamin E) and phylloquinone (vitamin K1) destruction under the effect of the day and ultra-violet light. The data obtained are presented. Phylloquinone and carotene proved to be most destroyed, while tocopherol was more resistant under the same conditions. Silufol plates can be used for the control of the production of vitamins, their analysis in varying biological objects, as well as in biochemistry, medicine and pharmaceutics.

Chromatography, Thin Layer↗
PubMedDate not supplied

[An improved method of determining the level of vitamin E in food products].

The author suggests a modified method for vitamin E estimation in foodstuffs. Thin-layer chromatography on Silufol (CSSR) plates was used for purification and isolation of vitamin E. Color reaction conducted directly on the plate makes possible increasing the estimation accuracy, especially when isomeric composition of vitamin E is studied in vegetable products. The use of excessive amounts of ethanol for alkaline saponification of samples enables estimation of vitamin E by the method suggested practically in all foodstuffs. The author has used this method for vitamin E estimation in some foodstuffs, in particular, in vegetable oils.

Animals↗