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Biomedical subjects

P E Cole

Publications and source records attributed to P E Cole.

At least 19 recordsLinked to original sources

Size fractionation of DNA fragments by liquid-liquid chromatography.

A method for the fractionation of double-stranded DNA fragments from 150 to 22000 b.p. in size by liquid-liquid chromatography is described. The procedure makes use of the fact that the partitioning of DNA in a polyethylene glycol-dextran system is size dependent and can be altered by alkali metal cations. Cellulose or celite are used as supports for the stationary, dextran-rich phase. Examples show the fractionation of digests of T7 DNA produced by Dpn II and Hind II restriction endonulceases as well as lambda DNA digests produced by Hind III and Eco RI restriction endonucleases.

Bacteriophage lambda

Sex discriminatory effectiveness using combinations of root lengths and crown diameters.

Using optical-scanner (OPTOCOM) and radiogrammetric measurements on mandibular permanent teeth, root length alone affords sex-discriminatory effectiveness equal to or exceeding conventional crown diameters. Combinations of root length and crown dimensions yield up to 80% accuracy in sexing with as few as two teeth, and discriminatory effectiveness of 87% with mandibular teeth alone.

Adolescent

Level of education, level of income, and level of fatness in adults.

Socioeconomic status is systematically related to the level of fatness, and therefore the incidence of obesity, in a total community survey of nearly 5,000 adults. Among males with more than 12 years of schooling, the average thickness of 4 fatfolds is 10% greater, amounting to about 2 kg of total fat, than those with 8 years or less of education. In females, however, the opposite trend is observed, those in the higher educational group averaging 20% thinner fatfolds, or about 5.5 kg total fat, than females in the lower educational group. These findings confirm the need for standards of obesity that take socioeconomic status into account.

Adipose Tissue