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Biomedical subjects

P E Brown

Publications and source records attributed to P E Brown.

At least 19 recordsLinked to original sources
PubMed1991

The partial amino acid sequence of the NAD(+)-dependent glutamate dehydrogenase of Clostridium symbiosum: implications for the evolution and structural basis of coenzyme specificity.

The amino acid sequence is reported for CNBr and tryptic peptide fragments of the NAD(+)-dependent glutamate dehydrogenase of Clostridium symbiosum. Together with the N-terminal sequence, these make up about 75% of the total sequence. The sequence shows extensive similarity with that of the NADP(+)-dependent glutamate dehydrogenase of Escherichia coli (52% identical residues out of the 332 compared) allowing confident placing of the peptide fragments within the overall sequence. This demonstrated sequence similarity with the E. coli enzyme, despite different coenzyme specificity, is much greater than the similarity (31% identities) between the GDH's of C. symbiosum and Peptostreptococcus asaccharolyticus, both NAD(+)-linked. The evolutionary implications are discussed. In the 'fingerprint' region of the nucleotide binding fold the sequence Gly X Gly X X Ala is found, rather than Gly X Gly X X Gly. The sequence found here has previously been associated with NADP+ specificity and its finding in a strictly NAD(+)-dependent enzyme requires closer examination of the function of this structural motif.

Amino Acid Sequence↗
PubMed1990

Exacerbation of chronic obstructive pulmonary disease due to hyperinfection with Strongyloides stercoralis.

Pulmonary infection due to the filariform larvae of Strongloides stercoralis may occur in immunocompromised patients residing in endemic areas of the United States. Such infection usually presents as dyspnea with a cough that sometimes results in bloody sputum. Although the chest roentgenogram often reveals a patchy bilateral alveolar infiltrate, acute respiratory distress is unusual. We report a patient who experienced severe exacerbation of his underlying obstructive lung disease that was associated with chest infiltrates and recovery of S stercoralis from his sputum. Although initial improvement was accomplished with Thiobendazole treatment, a re-exacerbation occurred when antiparasitic therapy was completed. The persistence of his infection is correlated to factors that are commonly employed in the treatment of COPD but may be overlooked as predisposing causes of hyperinfection with S stercoralis.

Animals↗
PubMed1987

Merkle cell carcinoma report of a case and possible role for adjuvant radiotherapy.

A case of neuroendocrine (Merkle cell) carcinoma of the skin is described. This uncommon tumor occurred in a 76-year-old female and presented as a small exophytic nodule on the right anterior chest wall. Following primary excision, it was thought to be a basal cell carcinoma. The diagnosis of Merkle cell carcinoma was established only after the tumor had recurred locally and involved ipsilateral axillary lymph nodes. Although local control was achieved with surgery and radiotherapy, the patient died of probable hepatic metastases 18 months after presentation. The Merkle cell tumor is an undifferentiated small cell carcinoma originating in the skin and has distinct ultrastructural characteristics that help to differentiate it from other small cell tumors. The clinicopathologic characteristics of these potentially aggressive neoplasms are reviewed, and treatment is briefly discussed.

Aged↗
PubMed1975

Ambulatory treatment of femoral shaft fractures with cast-brace.

Our early results support use of the cast-brace, early ambulation treatment of both open and closed fractures of the femoral shaft in all adult age groups. We believe initial traction should be used to reduce the fracture and maintain reduction until the fracture is clinically "sticky" to avoid subsequent malalignment in the cast-brace. The method facilitates early rehabilitation of the fractured extremity and the patient with minimal residual disability such as non-union, malunion, chronic infection and joint stiffness, so often associated with other forms of long bone fracture treatment.

Adolescent↗
PubMed1975

Surgical treatment for nonunion of the scaphoid.

Fracture of the scaphoid is the most common injury of the carpal bones. Most scaphoid fractures heal after immobilization. When the fracture does not unite, degenerative arthritic changes and disability may occur. Twenty-four symptomatic nonunions treated surgically were reviewed. Based on presence of union, percent of normal motion, and patients' symptoms, results were rated good in 19 cases and fair in five. Nine of ten patients treated with bone grafting and styloidectomy had union of fractures with good results. All five patients with bone grafting and internal fixation had union. Excision of proximal fragments of more than one third to one half of the scaphoid, and internal fixation alone, had unsatisfactory results. A styloidectomy should excise approximately one half of the radius articulating with the scaphoid. If not enough styloid is excised, osteophytic overgrowth will occur; if too much is excised, the carpus will sublux radially.

Adolescent↗
PubMed1972

The lipid-teichoic acid complex in the cytoplasmic membrane of Streptococcus faecalis N.C.I.B. 8191.

1. A lipid-teichoic acid complex was isolated from Streptococcus faecalis N.C.I.B. 8191. The covalent nature of the linkage between teichoic acid and lipid was established. 2. The complex exhibits macromolecular properties in solution, and ultracentrifugation studies show that these are due to micelle formation. 3. From chemical studies it is concluded that the teichoic acid is a poly(glycerol phosphate) in which some of the glycerol hydroxyl groups possess kojibiosyl [2-O-alpha-d-glucopyranosyl-(1-->2)-alpha-d- glucopyranosyl] substituents, together with d-alanine ester residues. 4. The lipid is 1-kojibiosyl diglyceride, already known as a membrane component of this organism, with probably a phosphatidyl substituent. The phosphatidyl kojibiosyl diglyceride is attached to the teichoic acid through a phosphodiester linkage, and the chain of the teichoic acid contains 28-35 units. 5. Although the complex represents the whole of the membrane teichoic acid in this organism, only about 12% of the membrane glycolipid is associated with teichoic acid. 6. Two phosphatidyl glycolipids, closely resembling that bearing the teichoic acid, were isolated from the lipids of the organism and were partly characterized.

Chromatography, Gas↗
PubMed1966

Thymine addition to ethanol: induction by gamma irradiation.

When thymine in dilute, deaerated, aqueous solution was irradiated with gamma-rays in the presence of ethanol, a high yield of products containing both the thymine and ethanol moieties was obtained. These were shown to be isomers formed by the attack of CH(3)CHOH radicals at the carbon No. 6 position of thymine. A similar reaction was observed with N, N'-dimethylthymine, but O,O'-dimethylthymine did not react. The reaction may be relevant to the resistance of certain cells to ionizing radiation.

Chemical Phenomena↗
PubMed1966

The leaf tannin of willow-herb [Chamaenerion angustifolium (L.) Scop.].

1. The leaf tannin of willow-herb [Chamaenerion angustifolium (L.) Scop.] has been isolated and separated into two fractions of differing solubility. 2. The tannin contains a penta-O-galloyl-beta-d-glucose core to which further galloyl groups are depsidically bound. 3. The unfractionated tannin contains an average of 10.5 galloyl groups/glucose molecule; the soluble fraction has on average 7.6 galloyl groups/glucose molecule and the less soluble fraction has 12.4. 4. The tannin is a mixture of molecules ranging at least from hepta- to trideca-galloyl-beta-d-glucose. 5. The tannin forms complexes with proteins and the fact that it is a hydrolysable gallotannin has a bearing on the release of nitrogen from the protein of the dead leaf.

Chemical Phenomena↗