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Biomedical subjects

P Duroux

Publications and source records attributed to P Duroux.

At least 145 records · Page 8Linked to original sources
PubMedDate not supplied

Low molecular-weight proteins in human gingival crevicular fluid.

Samples of gingival crevicular fluid (GCF) were collected in 30 volunteers with inflamed gingiva, using either capillary tubes (cGCF) or Durapore strips (sGCF). They were examined, together with samples of serum from the same patients, by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and/or by two-dimensional electrophoresis, followed by silver staining. The results confirmed that the distribution of the major proteins in GCF is similar to that found in serum. However, an 8.5 kDa protein was found in gingival fluid but not in serum. The low molecular-weight protein appears to decrease with time of fluid sampling with both techniques, and does not originate either from blood or from the cellular fraction of GCF. Two-dimensional electrophoretic analysis suggested that it may consist of several polypeptides.

Adult↗
PubMedDate not supplied

Antigenic release of paf-acether and beta-glucuronidase from alveolar macrophages of asthmatics.

Alveolar macrophages (AM) from control (n = 12) and atopic patients (n = 19, 12 without treatment, 4 treated with theophylline and 3 with theophylline and corticosteroid) were compared for their capacity to release mediators. AM were purified by 2 h adherence and challenged with either ubiquitous allergen, specific sensitizing allergens, anti-IgG or anti-IgE serum. The release of paf-acether, lyso paf-acether and beta-glucuronidase was measured. Paf-acether and its lyso derivative were assayed on washed rabbit platelets. Enzyme and mediator releases were obtained after specific allergenic or anti-IgE serum challenge of AM from untreated atopic patients. No release of paf-acether was detected from AM, from control or treated atopic patients after in vitro immunological challenge, whereas that of lyso paf-acether was greatly reduced in both treated groups. Release was obtained by immunological challenge of AM from control patients after passive sensitization with atopic serum. The release of mediators with bronchoconstriction activity by AM could represent an alternative causal pathway in human asthma.

Adult↗
PubMedDate not supplied

Evaluation of differential linear transformers as a non-invasive ventilatory method in supine subjects.

We present a new method to measure thoraco-abdominal perimeter variations using differential linear transformers inserted in belts. The high sensitivity (250 mV/mm), the low time drift (1 mV/24 h compared to a 1 V output for a tidal volume), the good frequency response (up to 10 Hz) and low cost (12,000 FF) are the major advantages over other devices. After calibration (using change contribution of thorax and abdomen and the least square method for calculation of volume-motion coefficients), estimated tidal volumes in 23 adults in supine position are within 0.06 +/- 5.17% and changes in functional residual capacity (43-453 ml) are within 3.4 +/- 15.3% when compared with direct spirometry. These results show that the method can be used to monitor ventilation accurately in supine healthy subjects.

Adolescent↗