Search PubMed⌕ Search

Biomedical subjects

P Draper

Publications and source records attributed to P Draper.

156 records · Page 9Linked to original sources
PubMedDate not supplied

Interaction of Mycobacterium leprae and mycobacteriophage D29.

This study of the interaction between Mycobacterium leprae and the mycobacteriophage D29 showed that the viruses caused a patchy damage of cell wall structure and the accumulation in the host of internal crystalline structures. Whether the observed ultrastructural alterations were caused by the replication of D29 was not clear. Mitomycin C also caused the accumulation of crystalline structures in M. leprae.

Cell Wall↗
PubMedDate not supplied

A study of alleged leprosy bacillus strain HI-75.

Subcultures of strain HI-75 of Skinsnes leprosy bacillus received in Antwerp and London have been studied bacteriologically and compared. Both contained moderately large acid-fast bacilli readily subcultured and maintained on ordinary mycobacteriologic media. These organisms were found to be a variety of Mycobacterium marianum (syn. serofulaceum) and were considered likely to be a laboratory contaminant. The earlier subculture studied also contained numbers of a much smaller mycobacterium (of a similar size to M. leprae) which appeared to be dead and which did not grow on the ordinary media. Skin tests and immunodiffusion analyses performed with extracts of the earlier subculture failed to demonstrate the presence of the specific antigens of leprosy bacilli. Similar studies on other cultures of Skinsnes bacillus must be performed to confirm or refute its identity as M. leprae.

Cell Wall↗
PubMedDate not supplied

Characterization of Mycobacterium leprae by lipid analysis.

The lipid composition of the leprosy bacillus, harvested from experimentally infected nine-banded armadillos, strongly supports it status as a distinct species of the genus Mycobacterium. Phthiocerol dimycocerosate waxes and glycosylated phenophthiocerol dimycocerosates are distinct from those characterised from a number of other mycobacteria. The polar lipids of a single isolate lack diacylated forms of phosphatidylinositol di- and pentamannosides, lipids usually found in most mycobacteria. A simple mycolic acid pattern composed of alpha-mycolates and ketomycolates is characteristic of most preparations of M. leprae.

Animals↗
PubMedDate not supplied

Host-grown Mycobacterium leprae: a credible microorganism.

In this paper recent advances in knowledge of the following aspects of Mycobacterium leprae will be reviewed: animal sources, ultrastructure, lipid composition, wall structure, molecular biology, metabolic capabilities and measurement of viability. It will be proposed that isolated from infected tissues are consistent, that is they share chemical and biochemical features although originating from many parts of the world, are unique, that is they have properties possessed by no other microorganism, and are biologically credible, meaning that their behaviour and properties are consistent with and sufficient for their postulated role as an intracellular pathogen. It is now clear that host-grown M. leprae is phenotypically quite distinct from organisms so far cultivated in vitro from infected tissues. The technique of DNA hybridization provides a method, unaffected by phenotypic changes brought about by transfer from a host to a medium, to compare host-grown M. leprae with cultivable microorganisms supposed to be genotypically identical.

Animals↗
PubMedDate not supplied

The mycolic acids of Mycobacterium leprae harvested from experimentally infected nine-banded armadillos.

Mycolic acid methyl esters were prepared from defatted cells of armadillo-derived Mycobacterium leprae and analysed by thin-layer and high-performance liquid chromatography, proton magnetic resonance spectrometry and mass spectroscopy. The first type of mycolic acid characterized was an "alpha-mycolate" having two cis-cyclopropane rings, a 78-carbon main component and an overall size-range of 72 to 83 carbons. Ketomycolates, with an 83-carbon main component, were the only other type of mycolate isolated; the major 79- to 87-carbon series of ketomycolates apparently contained a single trans-cyclopropane and the minor 80- to 86-carbon series had a cis-cyclopropane function.

Animals↗
PubMedDate not supplied

Cell walls of Mycobacterium leprae.

Walls were prepared from M. leprae isolated from livers and spleens of infected armadillos. These contained mycolic acids and arabinogalactan, but the composition of the peptidoglycan differed from that of the other mycobacteria that have been studied, since it contained substantial amounts of glycine. The relationship of M. leprae to the other mycobacteria remains obscure.

Animals↗
PubMedDate not supplied

Using traction.

Explore the source record for details and available documents.

Femoral Neck Fractures↗