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Biomedical subjects

P Draper

Publications and source records attributed to P Draper.

At least 109 records · Page 6Linked to original sources
PubMed1979

Single column high pressure liquid chromatographic determination of drugs in blood.

1. Analysis of anticonvulsants (phenobarbital, diphenylhydantoin and carbamazepine), theophylline and an antiarrhythmic agent (disopyramide) in blood using a simple high pressure liquid chromatography apparatus equipped with a reversed -- phase column is described. A simple extraction of plasma or serum with organic solvent is used to isolate the anticonvulsants and theophylline. Disopyramide is extracted with ether and is further purified by a back extraction into acid. 2. Hexanesulfonic acid -- methanol solutions are used for chromatography of the anticonvulsants and disopyramide while the mobile phase for theophylline is a NH4H2PO4 -- methanol mixture. Chromatographic analysis time for the drugs is approximately 15 minutes. The drugs are monitored by a UV detector at 254 nm except for theophylline which is measured at 280 nm. Quantitation is accomplished by comparison of peak heights with those of internal standards. Quantities of serum or plasma routinely used for analysis are: 200 ul for the anticonvulsants, 100 ul for theophylline and 0.5 ml for disopyramide. Detection limits are less than 1 ug/ml for these quantities.

Anticonvulsants↗
PubMed1979

Glucose tolerance and erythrocyte insulin receptors in Friedreich's ataxia.

Detailed in vivo and in vitro studies of glucose and insulin metabolism in Friedreich's ataxia patients and unaffected family members have further defined the extent of the abnormalities in carbohydrate metabolism. The high incidence of glucose intolerance and a hyperinsulinemic response to a glucose challenge in a high percentage of Friedreich's ataxia patients has been confirmed. An increased incidence of glucose intolerance among heterozygotes is suggested, while the siblings show a more normal distribution of diabetes and a nearly normal insulin response to the glucose tolerance test. Human growth hormone patterns are normal for all groups. Preliminary studies of insulin binding to erythrocytes suggest a difference in the binding characteristics among diabetic Friedreich's ataxia patients, while the binding in the non-diabetic Friedreich's ataxia group is similar to that of non-diabetic controls. Results from a small group of non-diabetic siblings suggest a normal insulin binding, while a tendency toward increased binding at low insulin concentrations among diabetic family members is noted.

Diabetes Complications↗
PubMed1979

Erythrocyte membrane lipids in Friedreich's ataxia.

In a study of the lipid composition of erythrocyte membranes in Friedreich's ataxia, the concentration of the major membrane components (phospholipids, cholesterol and protein) in ataxic patients, family members, and control subjects were found to be the same. The total fatty acid distribution was also normal. However, an altered distribution of phospholipid classes in erythrocytes was noted (an increase of PI + PS and a decrease of PE in Friedreich's ataxia patients).

Adult↗
PubMed1979

Effects of anionic inhibitors of phagosome-lysosome fusion in cultured macrophages when the ingested organism is Mycobacterium lepraemurium.

The mouse pathogen Mycobacterium lepraemurium is readily phagocytosed by cultured mouse peritoneal macrophages. Ingestion is normally followed by fusion between phagosomes and lysosomes. The influence of some aninonic compounds known to inhibit fusion in other systems was investigated by transmission electron microscopy after ingestion of M. lepraemurium. Fusion was markedly, although temporarily, inhibited by suramin and moderately inhibited by poly-D-glutamic acid. The effects are, however, not sufficient to permit these agents to be used to study the long-term effects of shutting off the secondary lysosome-phagosome fusion system in cultured macrophages infected with M. lepraemurium.

Animals↗
PubMed1978

Simple and rapid system for screening and identification of reducing sugars in urine.

As part of our screening programme for metabolic disorders we needed a rapid, simple, inexpensive means to detect reducing sugars in urine, with a simple but precise test for their identification. Our system comprises a bismuth reduction test for reducing sugars, with unidimensional thin layer chromatography on silica gel and color development with diphenylamine--aniline for definitive identification.

Carbohydrates↗
PubMed1978

Purine metabolism in Friedreich's ataxia.

In a detailed investigation of nucleotide synthesis, interconversion and degradation, no difference was found between subjects with Friedreich's Ataxia and normal controls. It appears improbable that this disorder is related to a primary defect in purine metabolism.

Adolescent↗
PubMed1977

Determination of the mass of Mycobacterium leprae by electron microscopy.

The mass of Mycobacterium leprae, obtained as a pure suspension from tissues of infected armadillos, was measured electron microscopically using a technique that avoids the need for standards of mass. The mean mass of an individual bacterium was 3-9+/-1-0 (S.D.) X 10(-14) g. Comparative measurements were also made on a small sample of M. lepraemurium (whose mass is known). Calculation of the mass of an individual bacterium allows numbers of bacteria in samples to be estimated by direct weighing rather than by counting.

Cell Count↗
PubMed1977

Heath & wealth.

Explore the source record for details and available documents.

Economics↗
PubMed1976

Growth of Mycobacterium lepraemurinum in the mouse bone marrow: an ultrastructural study.

The ultrastruct of the mouse bone marrow during the first 8 weeks after intravenous inoculation of animals with 10(9) Mycobacterium lepraemurium is described. The bacteria were almost exclusively in macrophages, which became converted to epithelioid cells after 8 weeks, at which time they were very heavily infected. The nature of the exceptionally rapid increase in numbers of bacteria in the bone marrow compared with other tissues early in the infection is discussed. It is concluded that a short doubling time of bacteria situated in the marrow is a more probable explanation than recruitment from elsewhere in the animal.

Animals↗
PubMed1975

The primary-care practitioner -- specialist or jack-of-all-trades.

Primary-care physicians, too often viewed as low-level generalists, are more appropriately thought of as specialists whose work demands specific skills. These physicians function as managers, advocates, educators, and counselors for their patients while also serving as co-ordinators of other professionals involved in primary care. The basis for education in primary care should be a recognition of the specialized nature of the work that primary-care physicians perform. Faculty members in primary-care programs should themselves be primary-care physicians; these educators deserve the same titles and status given other clinical specialists within the academic community. The setting of standards for primary-care practice and the design of research into areas such as the quality of care are tasks that should be undertaken by, or in collaboration with, physicians who are primary-care specialists.

Counseling↗
PubMed1973

Crowding among hunter-gatherers: the Kung Bushmen.

Highly crowded living conditions exist among the !Kung Bushmen, hunter-gatherers who live on the edges of the Kalahari Desert in Botswana and South-West Africa. The !Kung appear to be crowded by choice, and biological indicators of stress are absent. Data indicate that residential crowding alone does not produce symptoms of pathological stress.

Adult↗