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Biomedical subjects

P Destombes

Publications and source records attributed to P Destombes.

65 records · Page 4Linked to original sources
PubMedDate not supplied

[Changes in the positive P.A.S. reaction of the cytoplasm of giant cells. Role of etiologic factors and the giant cell reaction].

The search for alpha-amylase resistant, P.A.S. positive cytoplasm has been carried out in 206 giant cell lesions with or without specific inflammatory features and with neogenetic and degenerative plasmodia. It allowed to distinguish: --P.A.S. positivity in inflammatory cells of any nature, however with quantitative variations linked to etiologic factors (pathogen agent and disposition), to site, to the age of the plasmodia, and particularly, with negativation of the reaction in ancient lesions;--negativity of the neogenetic and degenerative plasmodia, save the giant cell articular lesions. Cells containing glycogen particularly (muscular tumors, renal or placental) are easily identified thanks to enzymatic digestion tests. Variations observed in the inflammatory cells seem to be the reflect of an active metabolism bringing about resorption phenomena but probably also immunization processes acting at the level of the cellular microenvironment.

Cytoplasm↗
PubMedDate not supplied

[Experimental infection of axenic mice by "Yersinia enterocolitica" (author's transl)].

Although most of Yersinia enterocolitica strains isolated from man have no pathogenicity for laboratory animals, it has been demonstrated that some strains are pathogenic for conventional mice and that most of the strains are probably pathogenic for Nude mice. The authors report the results of the infection of germ-free mice with a strain of Y. enterocolitica which is non pathogenic for holoxenic mice. It appears that C3H/He mice are sensitive to the infection by gavage or aerogenic and peritoneal routes. They all die within 8 to 12 days after injection of an inoculum of 5.10(5) viable cells. Germ-free NCS mice were also sensitive to the oral and aerogenic infection but not to the peritoneal infection; the difference between C3H/He and NCS sensitivity to this way of infection could be explained by a higher bactericidal activity of the peritoneal phagocytes of the latter. The C3H/He and NCS holoxenic control mice infected with the same inoculum of the same strain, did not show any symptoms and all attempts to isolate Y. enterocolitica failed three months after the challenge. Germ-free mice killed by the infection showed histopathological findings, i.e. abscesses involving intestinal wall. liver and spleen; they were similar to those described in experiments with pathogenic strains for conventional mice (holoxenic) and to those observed in infection of athymic Nude mice with strains non pathogenic for conventional mice. This infectious disease model is discussed in regards to the natural human infection.

Animals↗