Biomedical subjects

P Desjardins

Publications and source records attributed to P Desjardins.

At least 19 recordsLinked to original sources

PubMed2006

Ultrafast dynamics of delocalized and localized electrons in carbon nanotubes.

We report on the dynamics of the dielectric function of single-wall carbon nanotubes in the 10-30 THz frequency range after ultrafast laser excitation. The absence of a distinct free-carrier response is attributed to the photogeneration of strongly bound excitons in the tubes with large energy gaps. We find a feature of enhanced transmission caused by the blocking of optical transitions in small-gap tubes. The rapid decay of a featureless background with pronounced dichroism is associated with the increased absorption of spatially localized charge carriers before thermalization is completed.

Journal Article↗

PubMed2004

Docetaxel and Gemcitabine administered on days 1 and 8 for metastatic non-small cell lung carcinoma (NSCLC): a phase II multicenter trial.

Docetaxel and Gemcitabine are active agents in non-small cell lung carcinoma (NSCLC). They have different mechanism of action, minimal overlapping toxicity, and are easily administered on an outpatient basis. This phase II study evaluated Docetaxel administered with Gemcitabine on days 1 and 8 in a 3-week cycle, to determine its efficacy, while attempting to lower the regimen's toxicity, especially myelosuppression which can occur when Docetaxel is administered at full dose on day 1 only. Forty-three chemonaive patients, 40 evaluable, were entered in this trial between May 2001 and March 2002. Thirty-seven patients had stage IV and three patients had stage III B NSCLC, median age 58 (ages 32-78), median performance status (PS) 1 (range 0-2). They were treated with Docetaxel 36mg/m(2) and Gemcitabine 1000mg/m(2) intravenously on days 1 and 8 in a 3-week cycle. No growth factors were administered. Of 40 evaluable patients, 4 achieved partial response (10%), 25 stable disease (62.5%) and 11 progressive disease (27.5%). Median time-to-disease progression was 15 weeks. Median survival was 7.75 months. One year survival was 32.5% (13 patients). Hematologic toxicity was minimal, non-hematologic toxicity was easily treatable. Docetaxel, when given with Gemcitabine on days 1 and 8 every 3 weeks, is less myelotoxic, yet still an effective treatment for metastatic NSCLC.

Adult↗

PubMed2004

Back muscle fatigue during intermittent prone back extension exercise.

UNLABELLED: The purpose of this study was to estimate the level of muscular activation and muscle fatigue of the low back muscles during the performance of an intermittent prone back extension (PBE) exercise. Forty-one healthy students (24 males and 17 females) lying prone on a bench with the legs fixed performed two maximal voluntary contractions (MVC) in extension, a maximum of 100 repetitions of an intermittent PBE exercise immediately followed by a final MVC in extension. In addition, 12 subjects (11 males and one female) repeated the PBE exercise but with the addition of a 45-N weight on the back. The PBE exercise consisted of a task broken into four 1-s segments, while lying prone on a bench (10 degrees below horizontal): (1) raising the trunk to a horizontal position; (2) holding the trunk in the static phase (10 degrees above horizontal); (3) returning to the original position; and (4) resting on the bench. Electromyography (EMG) was used to measure the level of muscle activity (erector spinae (ES), gluteus maximus (GM), hamstrings (HA)) relative to the maximum voluntary EMG (MVE). RESULTS: Most of the subjects (34 out of 41) completed the 100 repetitions without excessive muscle fatigue according to the post-exercise MVC values. The intermittent PBE increased fatigue in the lumbar and hip extensor muscles in terms of: (1) a decrease in the MVC; (2) an increase in the level of muscle activation; and (3) a decline of the median frequency (MF). There was no gender difference in all EMG measurements. The level of muscle activation in the hip extensors (GM and HA) was associated with task failure (number of repetitions <100) for some subjects and the addition of a weight of 45 N had more impact on HA than ES. In conclusion, the PBE exercise as performed in the present study (including rest intervals), although not very strenuous for our healthy subjects, seems an adequate exercise to measure and train the aerobic capacity of the back muscles. However, to train specifically the back muscles, the exercise must be adjusted to avoid task failure due to possible hip extensor fatigue.

Adult↗

PubMed2002

Assessment of a three-dimensional robotic model for biomechanical-data acquisition of human movement.

The use of a kinematic robotic model has not been implemented in the biomechanical-data acquisition protocol, as it has in workplace analysis, ergonomics and design. The purpose of this paper was to assess the use of a kinematic model to retrieve frames of human movements from data obtained at a low sampling frequency. From experimental trials with an original sampling frequency of 60 Hz, the data were sampled again at two lower frequencies, 5 Hz and 10 Hz. The model was then used to reconstitute the data to its original frequency (60 Hz). The results demonstrated that it was possible to retrieve a full 3-D human movement from a sampling rate lower than normal without sacrificing accuracy. It was observed from both reduced sampling frequencies that the error level was comparable to the usual accuracy of a DLT 3-D reconstruction technique. It was therefore concluded that the data retrieved from these two frequencies were very similar to the original data sampled at 60 Hz.

Activities of Daily Living↗

PubMed2002

Handling missing marker coordinates in 3D analysis.

The use of mathematical formulations to retrieve missing data is not usually implemented as an automated computer procedure. The purpose of this paper is to present a simple mathematical formulation that can be used to retrieve the coordinates of markers hidden during a task. A strategy based on the smoothness of the variation of distance between points is devised, which leads to an automated computer procedure for retrieving hidden skin markers or missing joint coordinates (missing 3D points). This approach has the advantage of not being restricted to the usual rigid-body assumption.

Algorithms↗

PubMed2002

Estimated moments at L5/S1 level and muscular activation of back extensors for six prone back extension exercises in healthy individuals.

The purpose of this study was to identify the prone back extension (PBE) exercises that offer the most resistance to the back extensor muscles. Twenty males with no previous history of low back injury performed two repetitions of eight exercises. These consisted of two maximal isometric voluntary activations (MVA) and six different PBE exercises. The participants, while lying prone on a bench, were asked to raise either their trunk or legs to the horizontal position and hold for one second (static phase) before returning to the original position. The exercises differed according to the particular segments that were raised into extension. To assess the PBE exercises, a biomechanical model was used to estimate the level of load resistance (%MVA) relative to the maximum voluntary activation strength (MVA). In addition, electromyography (EMG) was included to measure the level of muscle activity (%MVE) relative to the maximum voluntary activation EMG (MVE) value reached during the MVA. A significant relationship of 0.86 was found between the mass of the subject and the peak reaction moment during the MVA. Peak level of load resistance averaged around 50% MVA with the exception of exercise no. 5 where the subject had to raise in extension his trunk, legs and arms (65% MVA). The erector spinae (ES) activity level reached a peak value of 61% MVE for exercise no. 5. In the exercises where only the trunk was lifted, the peak level of ES was below 47% MVE and the average activity during the static phase reached approximately 26% MVE. For healthy individuals PBE exercises can be considered low intensity exercises (< 50% MVA; except exercise no. 5) and can be used to improve the endurance of the back muscles.

Adolescent↗

PubMed2001

Alterations in expression of genes coding for key astrocytic proteins in acute liver failure.

Cerebral edema and hepatic encephalopathy are major complications of acute liver failure. Brain herniation caused by increased intracranial pressure as a result of cell swelling is the major cause of death in this condition. Evidence available currently suggests that the rapid accumulation of ammonia by the brain is the major cause of the central nervous system complications of acute liver failure. Increased brain ammonia may cause cell swelling via the osmotic effects of an increase in astrocytic glutamine concentrations or by inhibition of glutamate removal from brain extracellular space. Acute liver failure results in altered expression of several genes in brain, some of which code for important proteins involved in CNS function such as the glucose (GLUT-1) and glutamate (GLT-1) transporters, the astrocytic structural protein glial fibrillary acidic protein (GFAP) the "peripheral-type" benzodiazepine receptor (PTBR) and the water channel protein, aquaporin IV. Loss of expression of GLT-1 results in increased extracellular brain glutamate in acute liver failure. Experimental acute liver failure also results in post-translational modifications of the serotonin and noradrenaline transporters resulting in increased extracellular concentrations of these monoamines. Therapeutic measures currently used to prevent and treat brain edema and encephalopathy in patients with acute liver failure include mild hypothermia and the ammonia-lowering agent L-ornithine-L-aspartate.

Ammonia↗

PubMed2001

A force platform for large human displacements.

This paper provides details of a force platform assembly suitable for measuring the magnitude, position and direction of ground reaction forces for manual handling tasks involving several footsteps in any horizontal direction. An approach to increase the natural frequency with the use of a vibration absorber is used; this frequency was found to be 41+/-2 Hz. The results show a maximum error of 22 N (2% of the full scale output) when a person performs repetitive movements from a squat position to a full extension and back to the squat position. Static tests show the maximal errors to be, vertically, 1.5% of the full scale output (1818+/-33 N), and horizontally, 4.9% of the full scale output (1177+/-6 N).

Biomechanical Phenomena↗

PubMed2000

Pathway for the strain-driven two-dimensional to three-dimensional transition during growth of Ge on Si(001)

The two-dimensional (2D) to three-dimensional (3D) morphological transition in strained Ge layers grown on Si(001) is investigated using scanning tunneling microscopy. The initial step takes place via the formation of 2D islands which evolve into small ( approximately 180 A) 3D islands with a height to base diameter ratio of approximately 0.04, much smaller than the 0.1 aspect ratio of 105-faceted pyramids which had previously been assumed to be the initial 3D islands. The "prepyramid" Ge islands have rounded bases with steps oriented along <110> and exist only over a narrow range of Ge coverages, 3.5-3.9 monolayers.

Journal Article↗

PubMed2000

Strategies of load tilts and shoulders positioning in asymmetrical lifting. A concomitant evaluation of the reference systems of axes.

OBJECTIVES: To evaluate two handling factors on asymmetry of posture and efforts and to evaluate different reference systems of axes on the characterization of asymmetry.DESIGN. Ten novice workers performed three tasks evaluated for the effects of load tilting (tilts vs no tilts) and shoulders positioning (non-parallel vs parallel to the ground). Specific comparisons were made using three referential systems.BACKGROUND. Box handgrips/tilting and body posture are factors differentiating expert and novice workers which present a potential for reducing asymmetries. The literature also suggests that different conclusions may be reached about asymmetry with different systems of axes.METHODS. Net 3D moments at L5/S1 were obtained from two force platforms, four video cameras and inverse dynamic analyses using three reference systems of axes (trunk, pelvis, and joint).RESULTS. Tilting the load presented clear advantages of reducing the duration and trajectory of efforts, better positioning the load and reducing knee flexion, peak trunk extensors and mechanical work on the load. The maintenance of the shoulders parallel reduced asymmetries of posture and efforts but the results were affected by the type of reference system of axes chosen.CONCLUSIONS. Box tilting and shoulders positioning should be considered in the reduction of risk factors. More research is needed to define referential systems and characterize asymmetry. RelevanceBox tilting and shoulders positioning appear to be two fundamental elements to take into account in the formation programs based on prevention of risk factors. The importance of asymmetry among the risk factors emphasizes the need for better characterization of this risk.

Adult↗

PubMed1999

Chronic exposure of rat primary astrocyte cultures to manganese results in increased binding sites for the 'peripheral-type' benzodiazepine receptor ligand 3H-PK 11195.

Alterations of 'peripheral-type' benzodiazepine receptors (PTBRs) in brain are a feature of hepatic encephalopathy (HE). Although ammonia toxicity has been implicated in the disorder, recent findings suggest an accumulation of manganese in the brains of cirrhotic patients dying in hepatic coma. In this study, we examined the expression of PTBRs as well as the binding of the selective PTBR ligand 3H-PK 11195 in cultured astrocytes following chronic exposure to manganese. When astrocytes were exposed to 100 microM manganese for 1 week, a 57% increase in Bmax for 3H-PK 11195 binding was detected (P < 0.01) with no change in the Kd value. However, an examination by RT-PCR of the expression of the isoquinoline-binding moiety of the PTBR complex in these cells revealed no change in PTBR mRNA levels following manganese treatment. These findings suggest that manganese up-regulates 3H-PK 11195 binding sites by a process which does not involve a change in transcription. In view of the proposed role of astrocytic PTBRs in 'neurosteroid' synthesis, manganese-induced increases of PTBRs could contribute to the pathogenesis of HE.

Animals↗

PubMed1999

Increased expression of glyceraldehyde-3-phosphate dehydrogenase in cultured astrocytes following exposure to manganese.

Manganism is a disorder characterized by hyperintensities in basal ganglia structures on magnetic resonance imaging which may be the consequence of manganese deposition in these areas. Since manganese is taken up avidly into astrocytes and is known to interfere with cerebral energy metabolism, we studied the effect of this metal on the expression and activity of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in primary cultures of astrocytes. Treatment with 100 microM manganese for 7 days increased both the Vmax and Km values for GAPDH which was not reproducible with other divalent metals. Using RT-PCR, increased GAPDH expression was detected in cells exposed to manganese compared with controls. No changes in cytochrome oxidase activity or ATP levels were observed, and lactate production was unaffected, in manganese-treated cells. These findings provide evidence of a possible role for GAPDH in the mediation of the effects of manganese on central nervous system function.

Adenosine Triphosphate↗

PubMed1999

Portacaval anastomosis causes selective alterations of peripheral-type benzodiazepine receptor expression in rat brain and peripheral tissues.

There is a growing body of evidence to suggest that peripheral-type benzodiazepine receptors (PTBRs) and their endogenous ligands are implicated in the pathogenesis of end-organ failure in chronic liver disease. Portal-systemic encephalopathy, a major neuropsychiatric complication associated with chronic liver disease, results in activation of brain PTBR and probably in peripheral organs. In order to address these issues, PTBR mRNA was measured using semi-quantitative RT-PCR in extracts of cerebral cortex, kidney and testis of rats four weeks after end-to-side portacaval anastomosis and sham-operation (controls). Densities of PTBR sites were measured concomitantly by in vitro receptor binding using the selective PTBR ligand [3H]PK11195. Portacaval shunting resulted in a 2 to 3-fold increase in expression of PTBR in brain and kidney and a 37% reduction in expression in testis. Densities of [3H]PK11195 sites changed in parallel with the alterations of gene expression. These findings suggest that selective alterations of PTBR expression are implicated in the pathogenesis of peripheral tissue hypertrophy (kidney) and/or atrophy (testis) which accompanies portal-systemic shunting in chronic liver failure. In brain, activation of PTBR could result in an increase in the production of neurosteroids with potent inhibitory action in the CNS, which could contribute to the pathogenesis of portal-systemic encephalopathy.

Animals↗

PubMed1999

Increased "peripheral-type" benzodiazepine receptor sites and mRNA in thalamus of thiamine-deficient rats.

"Peripheral-type" benzodiazepine receptors (PTBRs) are highly expressed on the outer mitochondrial membrane of several types of glial cells. In order to further elucidate the nature of the early glial cell changes in thiamine deficiency, PTBR sites and PTBR mRNA were measured in thalamus, a brain structure which is particularly vulnerable to thiamine deficiency, of thiamine-deficient rats at presymptomatic and symptomatic stages of deficiency. PTBR sites were measured using an in vitro binding technique and the selective radio ligand [3H]-PK11195. PTBR gene expression was measured by RT-PCR using oligonucleotide primers based upon the published sequence of the cloned rat PTBR. Microglial and astrocytic changes in thalamus due to thiamine deficiency were assessed using immunohistochemistry and antibodies to specific microglial (ED-1) and astrocytic (GFAP) proteins respectively. Significant increases of [3H]-PK11195 binding sites and concomitantly increased PTBR mRNA were observed in thalamus at the symptomatic stage of thiamine deficiency, coincident with severe neuronal cell loss and increased GFAP-immunolabelling (indicative of reactive gliosis). Positron Emission Tomography using 11C-PK11195 could provide a novel approach to the diagnosis and assessment of the extent of thalamic damage due to thiamine deficiency in humans with Wernicke's Encephalopathy.

Animals↗

PubMed1999

Effect of portacaval anastomosis on glutamine synthetase protein and gene expression in brain, liver and skeletal muscle.

The effects of chronic liver insufficiency resulting from end-to-side portacaval anastomosis (PCA) on glutamine synthetase (GS) activities, protein and gene expression were studied in brain, liver and skeletal muscle of male adult rats. Four weeks following PCA, activities of GS in cerebral cortex and cerebellum were reduced by 32% and 37% (p<0.05) respectively whereas GS activities in muscle were increased by 52% (p<0.05). GS activities in liver were decreased by up to 90% (p<0.01), a finding which undoubtedly reflects the loss of GS-rich perivenous hepatocytes following portal-systemic shunting. Immunoblotting techniques revealed no change in GS protein content of brain regions or muscle but a significant loss in liver of PCA rats. GS mRNA determined by semi-quantitative RT-PCR was also significantly decreased in the livers of PCA rats compared to sham-operated controls. These findings demonstrate that PCA results in a loss of GS gene expression in the liver and that brain does not show a compensatory induction of enzyme activity, rendering it particularly sensitive to increases in ammonia in chronic liver failure. The finding of a post-translational increase of GS in muscle following portacaval shunting suggests that, in chronic liver failure, muscle becomes the major organ responsible for the removal of excess blood-borne ammonia.

Ammonia↗

PubMed1999

Toward a better prescription of the prone back extension exercise to strengthen the back muscles.

This study investigated the level of resistance and the level of muscle activation of the prone back exercise. Fifteen male subjects with no previous history of low back injury performed two repetitions of seven exercises. These consisted of four maximal isometric voluntary contractions (MVC) and three prone back extension (PBE) exercises. The subject was lying prone on a table, the upper body was suspended off the end of the table and the legs and thighs were secured to the table with straps. Three starting positions from the horizontal were investigated, 0 degree, 30 degrees and 60 degrees, and were compared with MVC to quantify the level of effort needed to perform the task. The results showed that the three PBE exercises require a level of resistance and a level of muscle activation generally under 40% of the maximum capacity of healthy subjects. Muscle activity of the erector spinae (ES) was slightly greater when the exercise started at 60 degrees, compared to 0 degree and 30 degrees. During the static phase of the PBE exercises, the level of resistance remained at 40% relative to the peak reaction moment of the MVC, but muscular activity of ES tended to work at a lower activity level. In conclusion, since for healthy subjects PBE exercises are low resistance exercises, they seem to be more specifically designed to develop muscular endurance of the back muscles.

Adult↗

PubMed1999

[Seroprevalence of hepatitis B and C viruses in a psychiatric institution].

Many patients admitted to psychiatric institutions have a history of risk factors for contamination with the hepatitis B and C viruses (HBV and HCV). Immunization policies for psychiatric institution patients are not widely know. A study conducted in a 600-bed psychiatric institution in Paris, France, to evaluate the proportion of potentially contaminating patients at admission, as well as immunization use in HBV-negative patients. Serologic markers for the HBV and HCV were looked for prospectively in all patients admitted over a two-month period. Immunization use was evaluated in the patients who had a first serologic test in 1995 or 1996 and follow-up tests until the end of 1997. The prospective part of the study demonstrated preadmission exposure to the HBV in 23.0 +/- 6.0% of patients. This proportion was larger in men (26.0%) than in women (14.8%), although the difference was not statistically significant (P = 0.10). Four patients (2.0%) tested positive for the HbS Ag. Among the HBV-negative patients, 13.0% received the vaccine; all had protective antibody levels. These patients were younger and more likely to be first-time admissions (18.4% vs 3.6%, P < 0.01). HCV seroprevalence was 6.0%. Serologic tests for the HBV were requested for 327 patients between January 1995 and december 1996. Among the patients who were seronegative at admission and received follow-up at the study hospital, only 13.8% were immunized at this hospital. Of the HCV-positive subjects, 63.3% were also HBV-positive. None of the HCV-positive HBV-negative subjects received immunization subsequently. Six to eight per cent of patients admitted to the study hospital are potentially contaminating for the HBV or HCV. The level of hepatitis B vaccine use is too low, particularly in high-risk patients. These data indicate a need for policies aimed at effectively preventing HBV and HCV transmission (information, education, immunization campaigns), both during the psychiatric institution stay and after discharge.

Adult↗

PubMed1998

Expression of ced-3 and ced-9 homologs in Alzheimer's disease cerebral cortex.

In order to characterize cell death mechanisms involved in Alzheimer disease (AD), we quantitated the expression of ced-3 and ced-9 homologs in AD frontal cortex. Positive (ICE, ICErel-II, ICErel-III, Ich-1L, CPP32, mch2, mch3, bcl-xS, bax and bak) and negative (bcl-2, bcl-xL, MCL1 and Ich-1S) regulators of apoptosis were successively examined using a semi-quantitative technique of reverse transcription-polymerase chain reaction (RT-PCR). Total RNA was extracted from postmortem frontal cortex of AD patients (n = 7) and controls (n = 7) matched for age and autolysis time. Baseline levels of message were detected for 3 ced-3 (CPP32, Ich-1 and ICE) and 4 ced-9 homologs (bcl-x, MCL1, bcl-2 and bax) in the frontal cortex. There was an overexpression of the ICEalpha cDNA in AD patients as compared with age-matched controls (P = 0.03). Our results indicate that several ced-3 and ced-9 homologs are expressed in the adult human brain, and suggest that neuronal cell death in AD might involve an aberrant expression of ICEalpha.

Adult↗