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P Das

Publications and source records attributed to P Das.

224 records · Page 13Linked to original sources
PubMedDate not supplied

Molecular mechanisms of pathogenesis in amebiasis.

Though both Entamoeba histolytica and E. dispar colonize the human gut, only the former is capable of invading tissues and causing disease. Although the biology of the parasite and the mechanism of pathogenesis have been intensively studied, there is a lack of consensus about the molecules of E. histolytica that actively participate in pathogenesis. This article reviews some key molecules involved. Ga1NAc-inhibitable adhesin is a membrane-associated glycoprotein nature, consisting of heavy and light subunits; each of these is encoded by multiple genes. The heavy subunit is useful in differentiating E. histolytica from E. dispar. Three structurally similar isoforms of amebapore, A, B and C, have been identified in E. histolytica but C is absent in E. dispar. Proteolytic enzymes such as collagenase and cysteine proteinases and cytolytic enzymes like phospholipase A are important. Collagenase activity is mainly accumulated in electron-dense granules. Cysteine proteinase is encoded by six genes, of which EhCP5 is exclusively present in E. histolytica.

Animals↗
PubMedDate not supplied

In vitro micropropagation of Lawsonia inermis (Lythraceae).

A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3% (w/v) sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light rather than the 14 hr photoperiod. Rooting was readily achieved upon transferring the microshoots onto MS basal semi-solid medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA) after ten days of culture. Micropropagated plantlets were acclimatized and successfully grown in soil.

Acclimatization↗
PubMedDate not supplied

Onset of in vitro rhizogenesis response and peroxidase activity in Zingiber officinale (Zingiberaceae).

The induction of rooting in microshoots of Zingiber officinale cvs. Suprava, Turia local, Suruchi and V3S18 was achieved on half-strength basal Murashige and Skoog's medium supplemented with 0.5-1.0 mg/l either indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) and 2% (w/v) sucrose within 7-9 days of culture. Rooting was inhibited when the microshoots were cultured under higher concentration of auxins. The microshoots cultured on medium supplemented with NAA induced large number of thin root hairs with friable calluses within 6-7 days. Peroxidase activity was determined during root induction (0-day to the 10th day at every 2 day interval) from microshoots derived in vitro. The activity was minimum in the inductive phase (primary) and at the maximum level during the root initiative phase. These finding may be useful in monitoring the rooting behaviour in microshoots derived from different subculture and peroxidase activity as a marker for root initiation.

Butyrates↗
PubMedDate not supplied

A study on intranatal care practices in a district of West Bengal.

A study was conducted at Birbhum district of West Bengal among mothers who delivered in last one year to generate area-specific community-based data on the proportion of home deliveries, assistance during conduction of delivery and the intranatal care practices followed in the district. A multistage 40 cluster sampling method was used to study 320 mothers in the district. 37.81% deliveries were conducted at home. About 25% deliveries were conducted by untrained birth attendants, unqualified practitioners or relatives and friends. In 85.13% of home deliveries, DDK was not used. 68.6% home deliveries were conducted on the floor without any clean covering sheet. Though a clean instrument was used to cut the cord in 86.78% of home deliveries, a clean cord tie was used in only 24.89% cases. In 36.36% home deliveries, something was applied on the cord stump. High proportion of deliveries assisted by untrained persons and high magnitude of faulty intranatal care practices observed in the study require urgent and appropriate intervention.

Cluster Analysis↗
PubMedDate not supplied

Multidrug resistant epidemic shigellosis in a village in west Bengal, 1984.

An out break of acute bacillary dysentery in a village called Dhamasin in Hooghly district of West Bengal was investigated during March 1984. Forty seven percent of families were affected. A total of 91 cases and 2 deaths occurred amongst 937 people giving an over all attack rate of 9.7% and a case fatality rate of 2.2 percent. Highest attack rate (22.7%) was observed in below one year age group. Multiple drug resistant Shigella dysentery type 1 strains were isolated for the first time from 6 out of 22 cases sampled at the domiciliary level. The organism was never isolated earlier during last ten years of surveillance in the infectious Diseases Hospital, Calcutta. Identification of nature of this outbreak and it's causative agent helped to realise the potentiality of extensive spread and paved the way for further investigations. Public health authorities were buffled as the rapid spread of the disease throughout the entire state of West Bengal could not be contained in spite of instituting all probable control measures on war footing.

Acute Disease↗