ON THE CONFORMATION OF HORSE HEART FERRI- AND FERROCYTOCHROME C.
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Biomedical subjects
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Conditions have been found under which polynucleotide phosphorylase adds only a few nucleotide residues to the 3' end of a dinucleotide primer. Pure tri- and tetranucleotides can be isolated from reaction mixtures in high yield.
The concentration of sodium chloride strongly influences primed polymerization of nucleoside diphosphates by polynucleotide phosphorylase. High concentrations of NaCl allow the addition of only a few nucleotide residues onto the 3' end of the primer. When pure oligonucleotides are used as primers and when the product is fractionated, pure block oligonucleotides can be obtained.
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