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Biomedical subjects

P Cox

Publications and source records attributed to P Cox.

106 records · Page 6Linked to original sources
PubMed1980

Evaluation of iron-chelating agents in cultured heart muscle cells. Identification of a potential drug for chelation therapy.

Primary cultures of neonatal rat cardiac muscle cells incorporated radioiron from both [55Fe]transferrin and 59FeCl3 (added simultaneously). To evaluate the effect of iron chelators on such uptake, deferri chelators were added 6 hr after addition of the radioiron sources. The microbial chelator agrobactin was significantly more effective than the drug defoxamine in reduction of 55Fe uptake from [55Fe]transferrin; both chelators halted 59Fe3+ uptake. Agrobactin may have potential in chelation therpay for iron-overload disease. Certain other microbial chelators lowered radioiron uptake from either [55Fe]transferrin of 59FeCl3. These chelators should be useful inhibitors for studies of animal cell iron uptake and intracellular iron flow.

Animals↗
PubMed1974

Prevention of bacteriophage adsorption to Staphylococcus aureus by immunoglobulin G.

Normal human and rabbit sera when incubated with Staphylococcus aureus inhibit the adsorption of bacteriophages. The bacteriophage adsorption was also inhibited by separated normal immunoglobulin M (IgM), F(ab')(2), and Fab-fragments of IgG. No inhibition was obtained with myeloma IgG or Fc-fragments of normal human and rabbit IgG. The results indicate that the serum inhibition of bacteriophage adsorption to S. aureus is not due to a binding of IgG to protein A on the surface of S. aureus.

Adsorption↗
PubMedDate not supplied

Are rectogenital chlamydial infections a marker of sexual abuse in children?

In order to examine the occurrence of chlamydial infection in sexually abused children, we cultured 51 abused children and 43 controls, 2 to 14.5 years of age. Only seven of the children were male. Chlamydia trachomatis was isolated from the vagina or rectum of two abused girls, 10 and 12 years of age, at their follow-up examination several weeks after the suspected abuse. C. trachomatis was also isolated from the vaginal culture of two controls and from the throat and rectum of another girl enrolled as a control. The two control children with vaginal infection were sisters, 7 and 10 years of age, who had been abused by their step-father 3 years previously. An antichlamydial immunoglobulin greater than or equal to 1:32 or a fourfold rise in the titer of immunoglobulin G was found in 13 of 49 (27%) abused children and 2 of 40 (5%) controls. This difference was statistically significant. The isolation of C. trachomatis from a rectogenital site was not limited to children with recent sexual abuse in this population. Serology may also be of limited use since serologic evidence of recent chlamydial infection was present in 5% of controls.

Adolescent↗
PubMedDate not supplied

Colonization of sexually abused children with genital mycoplasmas.

Although sexually abused children are usually evaluated only for the presence of infections with Neisseria gonorrhoeae and Treponema pallidum, they are also at risk for acquiring other sexually transmitted organisms prevalent in the adult population. Accordingly, we examined pharyngeal, anorectal and genital specimens from 50 children who had been sexually abused and from 40 healthy children who served as controls; these specimens were cultured for Mycoplasma hominis and Ureaplasma urealyticum. M. hominis was isolated from the anorectal and vaginal cultures of 11 (23%) and 16 (34%), respectively, of 47 abused girls as compared with three (8%) and six (17%), respectively, of 36 controls. U. urealyticum was isolated from the anorectal and vaginal cultures of nine (19%) and 14 (30%), respectively, of the abused girls as compared with one (3%) and three (8%), respectively, of 36 controls. Colonization with genital mycoplasmas was not associated with any symptoms.

Adolescent↗
PubMedDate not supplied

Children in critical care: how parents cope.

This article examines the parental role in the critical care setting. Parents' perceptions of stress and stressors are discussed, and the coping strategies developed by families to deal with them are identified. The implications of the findings are discussed in the light of current practice.

Adaptation, Psychological↗
PubMedDate not supplied

[High frequency oscillation ventilation compared to conventional mechanical ventilation plus exogenous surfactant replacement in rabbits]

OBJECTIVES: (a) to evaluate the effect on oxygenation and ventilation of rabbits with induced surfactant depletion when they are submitted to a conventional mechanical ventilation, plus a small dose of exogenous surfactant; (b) to compare this group with another group submitted to a High Frequency Oscillation (HFO) without exogenous surfactant administration.METHODS: Twenty New Zealand White rabbits weighing (-/+ 3 kg) were anaesthetized and artificially induced to a endogenous surfactant depletion by successively lung lavage with normal saline (aliquots of 25 ml/kg) until to reach a persistent PaO(2) less than 100 mmHg when submitted to a mechanical ventilation in a pressure control mode with a target tidal volume of 10ml/kg, PEEP of 5cm H(2)O, FiO(2) 1.0, respiratory rate 30/min, and inspiratory time of 0.65 s. Then the rabbits were divided in (a) CMV+S group, submitted to a conventional mechanical ventilation plus exogenous surfactant replacement; (b) HFO group, submitted to a High Frequency Oscillation Ventilation. Arterial blood gases were measured at control period, post lung lavage, 15, 16 and 120 minutes after treatment started. The groups were compared using Student t test.RESULTS: The post lung lavage PaO(2) in both groups was lower than 50mmHg (p=0.154), increasing after 15 min of treatment to 254 mmHg (CMV+S) and 288 mmHg (HFO, p=0.626). The PaO(2) at 60 and 120 minutes were higher (p=0.001) in the HFO group (431 e 431 mmHg) when compared with the CMV+S group, which showed a progressive fall (148 e 126 mmHg). At 60 minutes of treatment, the PaCO(2) was lower (p=0.008) in the CMV+S group (29 versus 41 mmHg).CONCLUSIONS: In ARDS animal model a protect mechanical ventilation strategy as HFO by itself promotes a fast and persistent increase in the oxygenation, with superior levels than those observed in animals treated with conventional mechanical ventilation plus exogenous surfactant replacement.

Journal Article↗