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Biomedical subjects

P Corne

Publications and source records attributed to P Corne.

27 records · Page 2Linked to original sources
PubMed1999

Detection and enumeration of HIV-1-producing cells by ELISPOT (enzyme-linked immunospot) assay.

The enzyme-linked immunospot (ELISPOT) assay was adapted to detect and enumerate HIV-1-producing cells at the single cell level. With CEM cells or peripheral blood mononuclear cells (PBMC) infected in vitro with HIV-1, the ELISPOT assay detected cells that produced HIV-1 antigens and showed that between 5.4% and 9.5% of the p24 antigen-positive CEM cells and 11.1% to 23.6% of the p24 antigen-positive PBMC were productively infected. In HIV-1-infected patients in early stage of the disease and without antiretroviral therapy, up to 4.54 HIV-1-producing cells per 10(6) CD4+ T lymphocytes were detected in peripheral blood and up to 277.75 HIV-1-producing cells per 10(6) CD4+ T lymphocytes were detected in splenic lymphoid tissue. Our results indicate that the ELISPOT assay could represent a new tool to study HIV-1 replication in vivo.

Adult↗
PubMed1998

[Infectious endocarditis on permanent endocavitary stimulation leads: apropos of 2 cases].

BACKGROUND: Permanent endocavitary pacemaker lead infectious endocarditis is rare. Two new cases are reported. CASE REPORTS: The first case appeared in a 63-year-old white woman with a permanent endocavitary pacemaker for 3 years. It was caused by Streptococcus bovis, unique to this pathology. The second case was caused by Streptococcus epidermidis in a diabetic 72-year-old woman. In these two cases, the clues to the diagnosis were hemocultures and transesophageal echocardiography. CONCLUSION: These two cases emphasize the importance of transesophageal echocardiography (TEE) for diagnosis and for driving treatment. Treatment is based on appropriate antibiotics and pacemaker removal. In the second, case, the material was surgically removed under cardiopulmonary bypass because of the size of the infection.

Aged↗