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Biomedical subjects

P Chand

Publications and source records attributed to P Chand.

90 records · Page 5Linked to original sources
PubMed1988

Counter-immunoelectrophoresis for the detection of Brucella antigen and antibodies in the diagnosis of brucellosis in buffaloes.

Counter-immunoelectrophoresis was employed for the detection of Brucella antigen in stomach contents of aborted buffalo fetuses and antibody in aborted as well as apparently healthy in contact buffaloes. Five of 16 aborted cases were serologically positive for brucellosis but isolation of Brucella abortus was successful in only two cases. By counter-immunoelectrophoresis, Brucella antigen was detected in the fetal stomach contents of four serologically positive cases. Of the 68 serum samples from in contact healthy buffaloes, 10 were positive with counter-immunoelectrophoresis: more than were detected by tube agglutination, Rose Bengal plate agglutination, complement fixation and agar gel precipitation test.

Abortion, Veterinary↗
PubMed1987

Detection of brucella antigen in bovine fetal stomach contents by agar gel precipitation and counter-immunoelectrophoresis.

Among 21 fetuses from serologically positive dams soluble brucella antigen was detected in the stomach contents in 14 instances by agar gel precipitation test and in 19 by counterimmunoelectrophoresis. Brucella abortus biotype 3 was isolated from the fetal stomach contents in 15 of these cases. None of 20 fetuses from serologically negative dams revealed brucella antigen in the stomach contents in either test.

Animals↗
PubMed1984

Synthesis and central nervous system activities of certain fluorine-containing 3-substituted indol-2-ones.

Several fluorine-containing 3- aroylmethyl -3-hydroxyindol-2-ones (4a-g), 3- aroylmethyleneindol -2-ones (5a-g) and 3- aroylmethylindol -2-ones (6a-g) were synthesized from the corresponding fluorine-containing indole-2,3-diones and appropriate ketones. The compounds were characterized by spectral studies. Representative compounds of each series were tested on mice for CNS activities, viz. analgesic and anticonvulsant and the effects were also observed against amphetamine-induced stereotypy, on conditioned avoidance response and on potentiation of pentobarbitone sodium hypnosis. Of the compounds examined, the greatest degree of activity was observed in 3- aroyl - methyleneindol -2-ones with a pronounced unsaturation in the system.

Analgesics↗
PubMed1982

Effects of phenobarbital and sodium salicylate on cytochrome P-450 mixed function oxygenase and glutathione S-transferase activities in rat brain.

The effects of acute and therapeutic doses of phenobarbital and sodium salicylate on cytochrome P-450 mixed function oxygenase (EC 1.14.14.1) and glutathione S-transferase (EC 2.5.1.18) activities have been studied in rat brain and compared with those of rat liver. P-450 enzymic activity was assayed by N-demethylation of p-chloro-N-methylaniline and 1-chloro-2,4-dinitrobenzene was used as substrate for glutathione S-transferase activity. The acute effects of a single daily dose of phenobarbital (75 mg/kg/day; i.p.) and sodium salicylate (500 mg/kg/day; i.p.) for 3 days increased cytochrome P-450 as well as glutathione S-transferase in rat liver. But the same doses of both drugs decreased glutathione S-transferase levels in rat brain and increased cytochrome P-450 dependent N-demethylation of p-chloro-N-methylaniline. The therapeutic doses of sodium salicylate (50 mg/kg/day; i.p.) and phenobarbital (10 mg/kg/day; i.p.) daily for 21 days increased cytochrome P-450 in rat liver as well as in brain. The increase in brain glutathione S-transferase by prolonged treatment of phenobarbital was significant compared to the control values.

Animals↗
PubMed1982

Triethyl lead toxicity in relation to brain glutathione and glutathione S-transferase.

A dose-dependent decrease in the content of reduced glutathione (GSH) was found in the brain of rats following treatment with Pb(Et)3 for varying time intervals. Maximum inhibition of 19% was observed 3 h after the last dose of Pb(Et)3 (8 mg/kg) daily for 3 days. Glutathione S-transferase activity did not change significantly. In vitro, there was no conjugation of GSH with PbEt3 and the addition of brain cytosol did not catalyse the conjugation.

Animals↗
PubMed1979

Possible psychopharmacological agents. Part 7: Synthesis and CNS activity of some fluorinated 2,4,7/8-trisubstituted-3H-1,5-benzodiazepinium monoperchlorates.

A series of new fluorinated 2,4'7/8-trisubstituted-3H-1,5-benzodiazepinium monoperchlorates have been synthesized and characterized by IR and NMR spectral studies. Representative compounds have been screened for their CNS activity viz., behavioural, analgesic, anticonvulsant and antireserpine. 8-Chloro-2-(4'-fluorophenyl)-4-phenyl-3H-1,5-benzodiazepinium monoperchlorate has shown pronounced antireserpine activity.

Analgesics↗
PubMedDate not supplied

Serum, zinc and copper levels in children with protein energy malnutrition.

Serum zinc and copper were measured by atomic absorption spectrophotometry in 58 children (3 months-5 years); of these, 46 children had protein energy malnutrition (PEM), and 12 children served as controls. The levels of serum zinc and copper were found to be significantly low in children with severe malnutrition (grades III and IV PEM). There was a significant positive correlation between serum zinc and height-for-age (r = 0.8809, p < 0.001). Serum copper was found low only in children exhibiting marked linear growth retardation (height-for-age < 85% of the normal). Hypoalbuminemia (serum albumin < 2.5 g/dl), and anemia (hemoglobin < or = 8.0 g/dl) in malnourished children were associated with significant decline in serum zinc and copper levels, respectively.

Biomarkers↗