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Biomedical subjects

P Cevc

Publications and source records attributed to P Cevc.

At least 19 recordsLinked to original sources
PubMed2001

A pulse EPR study of longitudinal relaxation of the stable radical in gamma-irradiated L-alanine.

The longitudinal relaxation rate of the first stable alanine radical, SAR1, was studied by employing pulse EPR technique over a wide temperature interval (5-290 K). The complex nonexponential recovery of the longitudinal magnetization in this temperature interval has been described with two characteristic relaxation times, 1/T*(1a) as the faster component and 1/T*(1b) as the slower component, respectively. It was shown that 1/T*(1a) is strongly affected by the CH(3) group dynamics of the SAR1 center. The complete temperature dependence of 1/T*(1a) was described by invoking several relaxation mechanisms that involve hindered motion of the CH(3) group from classical rotational motion to coherent rotational tunneling. It was shown that all relevant relaxation mechanisms are determined by a single correlation time with the potential barrier (Delta E/k=1570 K). On the other hand the temperature dependence of 1/T*(1b) is related to the motional dynamics of the neighborly NH(3) and CH(3) groups. We found a larger average potential barrier for this motion (Delta E/k=2150 K) corresponding to smaller tunneling frequencies of the neighbor groups.

Alanine↗
PubMed1999

The permeability of human cementum in vitro measured by electron paramagnetic resonance.

The structure and permeability of cementum are changed during the course of periodontal disease. In this study, the transport of water-soluble, spin-labelled molecules through cementum was studied by electron paramagnetic resonance (EPR). Cementum samples cut from different parts of the root were classified into four different groups: (A) samples exposed to the oral environment, (B) samples exposed to the periodontal-pocket environment; (C) samples cut from periodontally involved teeth but not exposed to saliva or periodontal pocket and (D) samples from sound young teeth extracted for orthodontic reasons. In order to obtain undamaged cementum, a dentine layer was left on each sample. Two methods were used to measure the diffusion coefficients of spin-labelled molecules in cementum dentine samples. First, the method of one-dimensional EPR imaging (EPRI) was used to evaluate the penetration of spin-labelled molecules into the cementum/dentine structure. Second, the diaphragm-cell method was used to determine the diffusion coefficients of the labelled molecules through the cementum under steady-state conditions. The results indicate that the interface between cementum and dentine is a barrier to diffusion. A set of diffusion (D) and partition (K) coefficients to describe the molecular transport in cementum, barrier and dentine was generated from the experimental data of both methods. For cementum (c), the barrier (b) and dentine (d) these coefficients were: Dc= 10(-8)cm2/s, Db= 10(-10)cm2/s, Dd= 10(-6)cm2/s and K=0.1. For the particular periodontally involved and uninvolved teeth the value of the rate-limiting barrier was DbA= 0.3 +/- 0.03 x 10(-10)cm2/s, DbB= 1 +/-0.3 x 10(-10)cm2/s, DbC= 0.3 +/- 0.03 x 10(-10)cm2/s, DbD= 0.4 +/- 0.05 x 10(-10)cm2/s. The largest diffusion flux across the dental hard tissue was found in the samples that had been exposed to the pocket environment (3.1 +/- 0.2) x 10(-9)cm2/s (p < 0.01), which coincided with the permeability calculated from the data evaluated by EPRI. The transport of the labelled molecules into and through the cementum dentine samples depends on the structure of the dental hard tissues, which changes during the course of periodontal disease. Knowledge of molecular diffusion across the tooth cementum/dentine structure is likely to be important for planning new treatments for periodontal disease.

Adolescent↗
PubMed1999

In vitro permeability and scanning electron microscopy study of acid-etched and ground enamel surfaces protected with dental adhesive coating.

Clinical procedures, such as acid etching and reshaping of the teeth supporting removable partial dentures by grinding off some enamel surface, increase the permeability of dental enamel. Teeth take several months in vivo to partially recover from such damage. In the meantime, the tooth is more susceptible to carious decay. To prevent this the ground or etched enamel should be effectively protected. Using electron paramagnetic resonance (EPR) and a two-chamber diffusion cell the authors studied the influence of adhesive resin applied to the ground and acid-etched enamel surfaces on the diffusion of spin label TMAPO (2,2,6-6 tetramethyl-4-acetamido-piperidine-1-oxyl) molecules through the enamel. The enamel permeability was measured in samples exposed to 1-min etching with 37% phosphoric acid, in samples etched for 5 min, and in samples ground with a diamond bur. Next, all the treated enamel surfaces were coated with Scotchbond Multi-Purpose Plus(R) dental adhesive system and the permeability measurements repeated. Scanning electron microscopy (SEM) was used to study the porosity of enamel surfaces. The adhesive resin film covering the etched or ground enamel surfaces was found to decrease significantly the diffusion through dental enamel. This finding confirms the clinical value of dental adhesives used to protect ground or accidentally acid-etched enamel surfaces. SEM analysis showed that adhesive resin covers the porous surface of the acid-etched and ground enamel tightly.

Acid Etching, Dental↗
PubMed1998

1-370 GHz EPR linewidths for K3CrO8: a comprehensive test for the Anderson-Weiss model.

Electron paramagnetic resonance (EPR) measurements have been carried out over the frequency range of 1-370 GHz on single crystals of potassium peroxychromate (K3CrO8) with the view of examining the current models of exchange narrowing of EPR signals in solids. K3CrO8 has a simple (tetragonal) lattice structure, can be grown as single crystals pure or diluted with an isostructural diamagnetic host K3NbO8, and its paramagnetism can be described by a very simple (S = 12, I = 0) spin Hamiltonian. The measurements were made at various orientations of single crystals in the Zeeman field, with emphasis on the principal directions of the g-tensor. For essentially all orientations, the linewidth decreases monotonically for measurements at resonance frequencies, omega0, from 1 to about 100 GHz, and then starts to increase at higher omega0. In order to delineate the spin exchange effects from other sources of line broadening, the measurements were repeated with a diluted spin system, K3NbO8 containing approximately/= 0.5 mole % of K3CrO8, representing the broadening effect of all the magnetic field dependent terms, such as the broadening due to the g-strain and sample holder/waveguide magnetization at the high field utilized, up to 14 T. Using these data, the K3CrO8 linewidths were analyzed in terms of the current models of spin exchange narrowing in three-dimensional systems. A reasonably good agreement was found with the Anderson-Weiss model, when modified for various line broadening effects. The accuracy of the analysis procedure was confirmed by the comparison of the presently determined values of the exchange constant, J, and the dipolar field, Hp, with their values obtained by dc magnetic susceptibility measurements and theoretical analysis, respectively; the agreement was within 5% for J (=1.35 K) and about 25% for Hp (160 G). However, some deviations and unusual splittings were noted in measurements at 370 GHz, whose origin remains unclear.

Chromates↗
PubMed1998

Crystallite arrangement of hydroxyapatite microcrystals in human tooth cementum as revealed by electron paramagnetic resonance (EPR).

Human dental cementum was analyzed by electron paramagnetic resonance (EPR). The measured EPR powder spectra of gamma-irradiated cementum resembled those of gamma-irradiated enamel. Both spectra were characterized by the same line shapes and g values. The position of the extreme first derivate peaks can be described by g1 = 2.0023 and g2 = 1.9971 +/- 0.0002, and are assignable to the CO3(3-) center. The angular dependence of the cementum EPR spectra indicates a different arrangement of the hydroxyapatite microcrystals compared to that of enamel. A corresponding model of cementum microcrystal alignment has been proposed. The methodology presented can be utilized for studying the mineralization process of root cementum and other mineralized tissues.

Adult↗
PubMed1997

Enhanced permeability of acid-etched or ground dental enamel.

STATEMENT OF PROBLEM: Acid etching creates retentive microcraters on enamel surfaces. Designing of a partial denture often involves reshaping the supporting and retentive teeth by grinding the enamel. Unfortunately, both these procedures damage the enamel surface. In vivo such surface damage takes several months to recover. PURPOSE: This study evaluated the effect of 1-minute etching, prolonged etching, and grinding on the permeability of dental enamel for water-soluble molecules. MATERIAL AND METHODS: With the electron paramagnetic resonance and a two-chamber diffusion cell, the influence of etching and grinding on the diffusion of spin label molecules through the enamel was studied quantitatively. The enamel permeability was measured in 30 sound enamel samples, of which 10 samples were exposed to 1-minute etching with 37% phosphoric acid, 10 samples were etched for 5 minutes, and 10 samples were ground with a diamond bur. RESULTS AND CONCLUSIONS: All procedures significantly increased the permeability of dental enamel. These results demonstrate that in vivo the acid-etched and ground dental enamel surfaces are less protected and consequently, unless the tooth is properly protected, are more susceptible to carious lesions. Therefore ground or accidentally etched enamel should be protected.

Acid Etching, Dental↗
PubMed1980

The caries resistance of human teeth is determined by the spatial arrangement of hydroxyapatite microcrystals in the enamel.

Teeth from different people differ in their susceptibility to caries. The beginning and the development of tooth decay depend on the presence of bacterial plaque and on the structure of the tooth enamel. But it has not been established whether the organic or the inorganic component of the enamel (which is comprised principally of hydroxyapatite microcrystals organized in a firm matrix), is responsible for most of the resistance of teeth to caries. We report here that the electron spin resonance (ESR) lines of the (CO3)3 defect of samples of enamel prepared from caries-resistant teeth differ significantly from the corresponding spectra of the caries-sensitive samples. We have traced these differences to the hydroxyapatite microcrystalline alignment in the tooth enamel, and found that this alignment is specific to individuals and is therefore probably nutritionally or genetically determined.

Crystallography↗