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Biomedical subjects

P Cesaro

Publications and source records attributed to P Cesaro.

3 recordsLinked to original sources

[Application to the study of connections in the CNS of the retrograde axonal transport of an iron-dextran complex].

The retrograde axonal transport of an iron-dextran complex was observed in neurons of the substantia nigra and of the intralaminar nuclei of the thalamus, after previous injection into the striatum. The histochemical demonstration of iron is simple and rapid, and can be combined with that of horseradish peroxidase, under precise conditions in the sequence of reactions. The iron-dextran complex revealed to be a valuable material for neuronal connectivity studies in the central nervous system.

Animals

Double labelling of blanched neurons in the central nervous system of the rat by retrograde axonal transport of horseradish peroxidase and iron dextran complex.

The retrograde axonal transport of an iron-dextran complex leads to a labelling of neural cell bodies in the central nervous system (CNS) of the rat. This tracer and horseradish peroxidase (HRP) can both be demonstrated histochemically in same cell bodies of intralaminar thalamic neurons in the central lateral nucleus, after injection of iron-dextran in the striatum and injection of HRP in the motor cortex. This is made possible by processing the sections first for HRP and then for ferric ions by Perl's reaction. This method allows an accurate demonstration of divergent axonal projections and is compatible with cytoarchitectonic studies on the same sections.

Animals

[Ketamine administration technic in pediatric anesthesia].

Psychic trauma connected with surgical operations and with all their preparatory procedures in children, often cause behavioural alterations. Problems related to the type of anesthetic premedication and to the anesthesiologic technique also come within this field. The Authors describe their experience with ketamine, administered by oral route, as a sole drug to obtain a deep sedation (when radiographic examination and orthopedic procedures were required) and as an induction drug for a balanced anesthesia.

Administration, Oral