Biomedical subjects
P Cabello
Publications and source records attributed to P Cabello.
Ph-positive chronic myeloid leukemia with t(8;21)(q22;q22) in blastic crisis.
A patient diagnosed with chronic myeloid leukemia was studied periodically during his illness. The result showed the presence of a Philadelphia (Ph) chromosome by a 9;22 translocation as a single abnormality to the time of blastic crisis. At that time, the chromosome studies showed a clonal evolution. Furthermore, a second derivated line was added to the Ph line. This new anomaly consisted of a 8;21 translocation, considered as specific of M2 type acute nonlymphoblastic leukemia of French-American-British classification.
[Use of a chromosome 21 gene library in the identification of a marker chromosome in chronic myeloid leukemia].
The non-isotopic in situ hybridization makes it possible the analysis of, both, numeric and structural chromosome aberrations in interphase nuclei. Moreover, this technique is useful for identification of chromosome markers of unknown origin, frequently present in malignant diseases. In our case, the fluorescent in situ hybridization allowed us, in a CML patient in accelerated phase, to know the origin of a chromosome marker, and then, to state that the patient had a 21 trisomy added to the Philadelphia chromosome.
Hydrogen peroxide is involved in hamster sperm capacitation in vitro.
We have investigated the possibility that the generation of hydrogen peroxide (H2O2) by spermatozoa plays a physiological role during capacitation. Capacitation is defined as the incubation period required for fertilization in mammals. Capacitation culminates in an exocytotic event, the acrosome reaction (AR). Mammalian sperm generate H2O2 during aerobic incubation and do not contain catalase, the enzyme that promotes scavenging of H2O2. In the present work we show that added catalase inhibited the AR, while glucose oxidase (GO), an enzyme that generates H2O2, accelerated the onset of the AR. Direct addition of H2O2 also stimulated the AR; catalase inhibited both the stimulation by GO and by H2O2. The onset of the AR was always preceded by the appearance of hyperactivated motility. The stimulation of the AR by H2O2 was manifest 1-2 h after the addition of H2O2. Catalase added at 3 h of incubation was less effective in inhibiting the AR than catalase added at the beginning. Incubation of sperm with catalase prevented the induction of the AR by the membrane-perturbing lipid, lysophosphatidyl choline. Taken together, these results suggest that H2O2 produced by hamster sperm plays a significant role during capacitation, possibly in membrane reorganization to facilitate the fusion that takes place during exocytosis of the acrosomal contents.
Association between XV2c/CS7/KM19/D9 haplotypes and the delta F508 mutation. A study of 57 Belgian families.
Using Southern blotting and the polymerase chain reaction, the prevalence of the haplotypes for XV2c, CS7, KM19 and D9 on CF and on normal chromosomes could be determined in 35 Belgian families. A set of primers complementary to the DNA sequence of the CF gene around the delta F508 deletion was used to amplify this particular segment of the gene. In a total of 57 families, deletion screening showed that 69 out of 116 CF chromosomes (59.5%) carried the delta F508 deletion. Both the delta F508 deletion and another mutation(s) showed strong association with the haplotype 1-2-2-2.
[Spontaneous subcapsular hematoma of the kidney associated with tubulopapillary neoplasm in a patient undergoing hemodialysis].
We report on a patient with chronic renal failure undergoing hemodialysis who presented with spontaneous renal subcapsular hematoma associated with a tubulo-papillary tumor. The literature relative to this condition is reviewed. Its incidence, predisposing factors, diagnostic methods and treatment are discussed.
[Mucinous adenocarcinoma of the prostate: presentation of 2 cases and review of the literature].
Explore the source record for details and available documents.
[Cavernosography in the diagnosis of Peyronie's disease].
Explore the source record for details and available documents.
[Extraperitoneal bladder rupture caused by diverticular pathology].
In this study we offer a case of spontaneous extraperitoneal vesical rupture and we analyse the etiopathogenic factors that are going to determine the genesis of the process, the diagnostic means used as well as the therapeutic attitude to be followed on the basis of the patient's clinical picture and the bladder level where the rupture has occurred.
[Application of transmission and scanning electron microscopy in experimental obstructive uropathy: I. Glomerular lesions].
In this research we use the Electronic Transmission and Sweep Microscope to study the morphostructural modifications undergone by the renal glomerulus in experimental obstructive uropathy. The most significant alterations take place at the level of the basal lamina of the glomerulus, where progressive thickening anf folding occurs. The podocytes display, mainly in the late groups, loss of the digit-shaped character of their secondary prolongations, with shortening and thickening of these. They also take on an anarchic arrangement and are pseudofused to one another, forming a cellular lamina in contact with the underlying basal membrane. These morphostructural changes justify the reduction in glomerular filtration that takes place in obstructive uropathy.
[Application of transmission and scanning electron microscopy in experimental obstructive uropathy: II. Tubular lesions].
The morfoestructural changes that appear at renal tubule level in obstructive uropathy consist in a progressive loss of the differentiations in the plasmatic membrane: reduction in the number of apical microvilli and of basal invaginations, which entails a drop in the exchange surface area. Degeneration and cellular necrosis phenomena also exist, with the appearance of cell traces in the tubule lumen. On the other hand, morphological signs of a rapid rate of cellular activity in the tubule cells are displayed. Together with the modifications that take place at basal membrane level these changes give rise to an alteration of tubule dynamics.
[Application of transmission and scanning electron microscopy in experimental obstructive uropathy: III. Lesions at the interstitial level].
In experimental obstructive uropathy the renal interstice undergoes profound modifications, from the earliest stages, with the fibroblastic elements adopting a palisade arrangement between the renal tubules and the blood cells. Between these cells there is a marked edema configuring "canals", which could be the morphological image of the tubulovenous reflux described in obstructive uropathy. these routes, which we will call tubule-interstice-vein "canals", since in no case could we verify the existence of a direct communication between the tubule formations and the veins, disappear in animals put down late, where fibro and myofibroblastic elements, round cells and macrophages proliferate. A larger number of fibres and fundamental substance are also found. As a result of these changes, a great alteration in renal architecture takes place.
[Cryptorchidism, orchidopexy and testicular cancer: report of a case showing this association].
Explore the source record for details and available documents.
[Trousseau's syndrome: thrombosis of the inferior vena cava and of the left renal vein associated with a testicular neoplasm].
Explore the source record for details and available documents.
[Melanoma of the glans penis: report of a case and review of the literature].
Explore the source record for details and available documents.
[Paratesticular rhabdomyosarcoma. Presentation of a case and discussion of the biology of these neoplasms].
Explore the source record for details and available documents.